阿拉伯宝螺来源真菌-细菌共生体Aspergillus spelaeus GXIMD 04541 / Sphingomonas echinoides GXIMD 04532的次级代谢产物研究

doi:10.11978/2024104

Abstract

Abstract:

In order to investigate the secondary metabolites of the fungal-bacterial symbiont Aspergillus spelaeus GXIMD 04541 / Sphingomonas echinoides GXIMD 04532 derived from Mauritia arabica, a series of isolation and purification techniques, including silica gel chromatography, gel column chromatography, and semi-preparative high performance liquid chromatography (HPLC), were employed. The structures of compounds were identified by spectroscopic analysis and literature comparison. Cytotoxic activity evaluation of compounds was conducted using the MTT (Methylthiazolyldiphenyl tetrazolium bromide) assay. Consequently, seven compounds were obtained from rice fermentation extracts of symbiont GXIMD 04541 / 04532, which were identified as trichalasins C (1), aspochalasin E (2), aspochalasin H (3), aspochalasin I (4), aspochalasin K (5), citreofuran (6), and cyclothiocurvularin B methyl ester (7). Activity assays demonstrated that compounds 2, 4, and 5 exhibited cytotoxic activity against PC3 cells, with IC₅₀ (half maximal inhibitory concentration) values of 17.23, 15.18 and 8.71 μmol·L^-1, respectively. However, compounds 1-5 showed no cytotoxicity against 22Rv1 cells.

Key words: Mauritia arabica, fungal-bacterial symbiont, secondary metabolites, cytotoxicity

CLC Number:

P735.2

YANG Jie, YAO Feihua, LI Xiaoyan, SHI Jieyu, YI Xiangxi, GAO Chenghai. Secondary metabolites from the fungal-bacterial symbiont Aspergillus spelaeus GXIMD 04541 / Sphingomonas echinoides GXIMD 04532 derived from Mauritia arabica[J].Journal of Tropical Oceanography, 2025, 44(2): 56-63.

Figures/Tables 7

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References 26

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化合物	IC₅₀/(μmol·L^-1)
1	> 20
2	17.23
3	> 20
4	15.18
5	8.71
多柔比星	5.09

Secondary metabolites from the fungal-bacterial symbiont Aspergillus spelaeus GXIMD 04541 / Sphingomonas echinoides GXIMD 04532 derived from Mauritia arabica

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