Marine biology

Effect of different levels of NaHCO3, NaCl and pH on the growth of a
EPA-producing diatom strain

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  • 1. South China Sea Institute of Oceanology, CAS, Guangzhou 510301, China; 2. Graduate School of the CAS, Beijing 100049, China
苏娇娇(1982—), 女, 河北省晋州市人, 硕士研究生, 主要从事微藻生物技术研究。E-mail: sjjsusan@gmail.com

Received date: 2010-03-25

  Revised date: 2010-04-29

  Online published: 2010-12-15

Supported by

广东省海洋与渔业科技项目(A200899I01); 广东省科技计划项目(2009B080701093)

Abstract

 In this study, we investigated the composition and content of fatty acids, and the effects of different levels of Na-HCO3, NaCl and pH on an alkalophilic diatom strain, Nitzschia sp. MD1. We showed that MD1 contained 3.02% EPA in dry weight biomass, which was 17.93% of the total fatty acids. It was indicated that the optimal range of NaHCO3 for MD1 growth was between 0.1?0.2 M, and that MD1 showed a good growth even when NaHCO3 was below 0.3. The optimal range of initial pH for MD1 growth was between 7.5 and 9.5, and that MD1 maintained a considerable growth even when initial pH was 11.5. It was also shown that MD1 exhibited adaptation to a wide range of salinity, which brought about no obvious difference in the growth of MD1 when NaCl was between 0?40g•L?1. The maximum specific growth rate of MD1 reached 0.56d ?1 at 0.2mol•L?1 of NaHCO3, 30g•L?1 of NaCl, and 8.5?9.5 of pH. These results present a prospective future for large-scale cultivation and EPA production of MD1.

Key words: Nitzschia; alkalophilic; pH; NaHCO3; EPA.

Cite this article

SU Jiao-jiao,XIANG Wen-zhou,XIAO Bei,XU Shao-kun,HE Hui . Effect of different levels of NaHCO3, NaCl and pH on the growth of a
EPA-producing diatom strain[J]. Journal of Tropical Oceanography, 2010
, 29(6) : 79 -85 . DOI: 10.11978/j.issn.1009-5470.2010.06.079

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