菱形藻株系MD1的脂肪酸组成及其在NaHCO3、NaCl和pH值影响下的生长特征

doi:10.11978/j.issn.1009-5470.2010.06.079

Abstract

Abstract:

In this study, we investigated the composition and content of fatty acids, and the effects of different levels of Na-HCO3, NaCl and pH on an alkalophilic diatom strain, Nitzschia sp. MD1. We showed that MD1 contained 3.02% EPA in dry weight biomass, which was 17.93% of the total fatty acids. It was indicated that the optimal range of NaHCO3 for MD1 growth was between 0.1?0.2 M, and that MD1 showed a good growth even when NaHCO3 was below 0.3. The optimal range of initial pH for MD1 growth was between 7.5 and 9.5, and that MD1 maintained a considerable growth even when initial pH was 11.5. It was also shown that MD1 exhibited adaptation to a wide range of salinity, which brought about no obvious difference in the growth of MD1 when NaCl was between 0?40g•L?1. The maximum specific growth rate of MD1 reached 0.56d ?1 at 0.2mol•L?1 of NaHCO3, 30g•L?1 of NaCl, and 8.5?9.5 of pH. These results present a prospective future for large-scale cultivation and EPA production of MD1.

Key words: Nitzschia, alkalophilic, pH, NaHCO3, EPA.

SU Jiao-jiao,XIANG Wen-zhou,XIAO Bei,XU Shao-kun,HE Hui. Effect of different levels of NaHCO3, NaCl and pH on the growth of a
EPA-producing diatom strain[J].Journal of Tropical Oceanography, 2010, 29(6): 79-85.

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Effect of different levels of NaHCO3, NaCl and pH on the growth of a
EPA-producing diatom strain

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Effect of different levels of NaHCO3, NaCl and pH on the growth of a EPA-producing diatom strain

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Effect of different levels of NaHCO3, NaCl and pH on the growth of a
EPA-producing diatom strain