The bacterial strain HSY-03 is closely related to the genus Bacillus sp. and capable of inhibiting the toxic dinoflagellate Heterosigma akashiwo. It was isolated from the mangrove area in Yunxiao, Fujian Province, China. Based on 16S rRNA gene sequences and morphological characteristics, the strain HSY-03 was determined to be Bacillus sp. on the basis of 99.0% similarity with reference strain sequences from the NCBI. HSY-03 indirectly attacked Heterosigma akashiwo by secreting extracellular substances. The algicidal compounds could not be proteinaceous, nucleate and polysaccharide, which showed heat tolerant and being stable in UV as well as acidic and alkaline conditions. The bacterial strain was species-specific among the 13 algae tested. There was a close interaction between initial bacterial and algae cell densities. Bacterial HSY-03 showed strong algicidal activity when the initial concentration was above 5.0%. HSY-03 showed certain algicidal effects on Heterosigma akashiwo during different growth periods, especially during its stable and decline phases. These findings provided a novel viewpoint on biological control of harmful algal blooms and the relationship between the bacteria and algae.
FU Lijun
,
LIN Tiancheng
,
YU Xiaoqi
,
ZHENG Tianling
. Isolation and identification of algicidal bacteria HSY-03 and its impact on Heterosigma akashiwo[J]. Journal of Tropical Oceanography, 2016
, 35(3)
: 87
-93
.
DOI: 10.11978/2015099
1 东秀珠, 蔡妙英, 2001. 常见细菌系统鉴定手册[M]. 北京: 科学出版社. DONG XIUZHU, CAI MIAOYING, 2001. Common bacterial identification manual[M]. Beijing: The Science Publishing Company (in Chinese).
2 郭皓, 2004. 中国近海赤潮生物图谱[M]. 北京: 海洋出版社. GUO HAO, 2004. Illustrations of planktons responsible for the blooms in Chinese coastal waters[M]. Beijing: Maritime Press (in Chinese).
3 徐玲玲, 2010. 一株赤潮异弯藻溶藻细菌的分离与溶藻特性研究[D]. 武汉: 华中师范大学. XU LINGLING, 2010. Isolation and algae-lysing characteristics of the algicidal bacterium against Heterosigma akashiwo [D]. Wuhan: Central China Normal University (in Chinese).
4 王年斌, 周遵春, 马志强, 等, 2006. 大连湾赤潮异弯藻赤潮的多元分析[J]. 海洋学报, 28(3): 151-156. WANG NIANBIN, ZHOU ZUNCHUN, MA ZHIIANGQ, et al, 2006. Analysis on multivariate statistics for Heterosigma akashiwo blooming in Dalian Bay[J]. Acta Oceanologica Sinica, 28(3): 151-156 (in Chinese).
5 ANDERSON D M, 1997. Turning back the harmful red tide[J]. Nature, 388: 513-514.
6 BUTRON A, MADARIAGA L, ORIVE E, 2012. Tolerance to high irradiance levels as a determinant of the bloom-forming Heterosigma akashiwo success in estuarine waters in summer[J]. Estuarine, Coastal and Shelf Science, 107: 141-149.
7 DOCKYU K, KIM J F, YIM J H, et al, 2008. Red to red - The marine bacterium Hahella chejuensis and its product prodigiosin for mitigation of harmful algal blooms[J]. Journal of Microbiology Biotechnology, 18(10): 1621-1629.
8 FU LIJUN, AN XINLIN, LI DONG, et al, 2011. Isolation and alga-inhibiting characterization of Vibrio sp. BS02 against Alexandrium tamarense [J]. World Journal of Microbiology Biotechnology, 27(12): 2949-2956.
9 GUAN C W, GUO X Y, CAI G J, et al, 2014. Novel algicidal evidence of a bacterium Bacillus sp. LP-10 killing Phaeocystis globosa , a harmful algal bloom causing species[J]. Biological Control, 76: 79-86.
10 LI Y, ZHU H, GUAN C W, et al, 2014. Towards molecular, physiological, and biochemical understanding of photosynthetic inhibition and oxidative stress in the toxic Alexandrium tamarense induced by a marine bacterium[J]. Applied Microbiology Biotechnology, 98(10): 4637-4652.
11 LI YI, ZHU HONG, LEI XUEQIAN, et al, 2015. The death mechanism of the harmful algal bloom species Alexandrium tamarense induced by algicidal bacterium Deinococcus sp.Y35[J]. Frontiers in Microbiology, 6(992): 1-15.
12 LUO J, WANG Y, TANG S. et al, 2013. Isolation and identification of algicidal compound from streptomyces and algicidal mechanism to Microcystis aeruginosa [J]. PLoS One, 8(10): 1-14.
13 MAYALI X, AZAM F, 2004. Algicidal bacteria in the sea and their impact on algal blooms[J]. Journal Eukaryotic Microbiology, 51(2): 139-144.
14 SHIKATA T, NAGASOE S, MATSUBARA T, et al, 2008. Factors influencing the initiation of blooms of the raphidophyte Heterosigma akashiwo and the diatom Skeletonema costatum in a port in Japan[J]. Limnology Oceanography, 53(6): 2503-2518.
15 SU J Q, YANG X R, ZHENG T L, et al, 2007. An efficient method to obtain axenic cultures of Alexandrium tamarense - A PSP producing dinoflagellate[J]. Journal of Microbiological Methods, 69(3): 425-430.
16 WANG B X, YANG X R, ZHOU Y Y, et al, 2012. An algicidal protein produced by bacterium isolated from the Donghai Sea, China[J]. Harmful Algae, 13: 83-88.
17 ZHENG XIAOWEI, ZHANG BANGZHOU, ZHANG JINLONG, et al, 2013. A marine algicidal actinomycete and its active substance against the harmful algal bloom species Phaeocystis globosa [J]. Applied Microbiology Biotechnology, 97(20): 9207-9215.
