抑藻细菌HSY-03的分离、鉴定及对赤潮异弯藻生长的影响

doi:10.11978/2015099

Journal of Tropical Oceanography ›› 2016, Vol. 35 ›› Issue (3): 87-93.doi: 10.11978/2015099CSTR: 32234.14.2015099

• Marine Biology • Previous Articles Next Articles

Isolation and identification of algicidal bacteria HSY-03 and its impact on Heterosigma akashiwo

FU Lijun¹, LIN Tiancheng¹, YU Xiaoqi², ZHENG Tianling²

. College of Environment and Life Science, Fujian Provincial Key Laboratory of Ecology-toxicological Effects & Control for Emerging Contaminants, Putian University, Putian 351100, China;
2. Key Laboratory of Ministry of Education for Coast and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, China

Received:2015-07-28 Online:2016-05-29 Published:2016-05-27
Contact: FU Lijun. E-mail: lijun_fu@sina.com
Supported by:
National Nature Science Foundation (31400318); New Century Excellent Talents in Fujian Province University; Key Projects of Science and Technology Department of Fujian Province (2013N0030); Science and Technology Project of Putian (2012S04)

Abstract

Abstract: The bacterial strain HSY-03 is closely related to the genus Bacillus sp. and capable of inhibiting the toxic dinoflagellate Heterosigma akashiwo. It was isolated from the mangrove area in Yunxiao, Fujian Province, China. Based on 16S rRNA gene sequences and morphological characteristics, the strain HSY-03 was determined to be Bacillus sp. on the basis of 99.0% similarity with reference strain sequences from the NCBI. HSY-03 indirectly attacked Heterosigma akashiwo by secreting extracellular substances. The algicidal compounds could not be proteinaceous, nucleate and polysaccharide, which showed heat tolerant and being stable in UV as well as acidic and alkaline conditions. The bacterial strain was species-specific among the 13 algae tested. There was a close interaction between initial bacterial and algae cell densities. Bacterial HSY-03 showed strong algicidal activity when the initial concentration was above 5.0%. HSY-03 showed certain algicidal effects on Heterosigma akashiwo during different growth periods, especially during its stable and decline phases. These findings provided a novel viewpoint on biological control of harmful algal blooms and the relationship between the bacteria and algae.

Key words: algicidal bacteria, harmful algal blooms, Heterosigma akashiwo, extracellular substances

CLC Number:

P735.53

FU Lijun, LIN Tiancheng, YU Xiaoqi, ZHENG Tianling. Isolation and identification of algicidal bacteria HSY-03 and its impact on Heterosigma akashiwo[J].Journal of Tropical Oceanography, 2016, 35(3): 87-93.

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Isolation and identification of algicidal bacteria HSY-03 and its impact on Heterosigma akashiwo

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