Journal of Tropical Oceanography >
Verification of QTL and genotype selection effect of QTL for growth-related traits in the Pearl Oyster, Pinctada fucata
Received date: 2016-07-28
Request revised date: 2016-09-12
Online published: 2017-04-06
Supported by
Marine Fishery Science and Technology Development Project in Guangdong Province (Z2014014, Z2015014)
Science and Technology Plan Project of Guangdong Province (2014B030301064)
Copyright
QTL411871 was identified in the verifying population of Pinctada fucata, and two genotype matings♀AT×♂AT(Group No.30 ) and ♀TT×♂AT(Group No.31) were established to analyze genotype selection effect of QTL. The results showed that QTL411871 was significantly associated with muscle mass in the verifying population (P<0.05), and AT was the superior genotype. QTL411871 was not significantly associated with growth traits in Groups No. 30 and No. 31 (P>0.05); the genetic effect of AT genotype was small, but it was still the genotype with the maximal average of growth traits. QTL423886 was significantly associated with shell height, shell length and total weight in Group No. 30 (P<0.05), and the oysters with genotype AG were bigger than those with the genotype GG in terms of shell height, shell length and total weight, but it was not significantly associated with growth traits in Group No.31 (P>0.05). Diplotypes were constructed based on the two QTLs, and association analyses revealed that C1 (ATAG) in Group No.30 and D1 (ATAA) in Group No.31 might be the most advantageous diplotypes for growth traits. These results are useful for developing molecular marker assisted breeding of Pinctada fucata.
WEI Guojian , LI Yaoguo , LIN Jianshi , HE Maoxian . Verification of QTL and genotype selection effect of QTL for growth-related traits in the Pearl Oyster, Pinctada fucata[J]. Journal of Tropical Oceanography, 2017 , 36(2) : 19 -25 . DOI: 10.11978/2016072
Tab. 1 Primers used for analysis of QTL411871表1 QTL411871标记SNP分型引物 |
引物 名称 | 序列(5'-3') | 退火 温度/℃ | 等位基因 | 对应产物/bp |
---|---|---|---|---|
FI | GGAAACGGAATTCACATTCATTATCTTTA | 64 | A | 200 |
RI | AATGTCAAGCTGGATATAATTTAGCTCTA | 60 | T | 171 |
FO | AAATTATTGAAACTATTTCACCGATTCG | 62 | 空白 | 空白 |
RO | TTGTGATTTCATTTTCGTATCAATATTCTT | 62 | 空白 | 空白 |
Tab. 2 Genetic parameters of QTLs in P. fucata表2 QTL标记的遗传参数 |
群体/组配 | QTL | PIC | Ho | He | HWE P值 |
---|---|---|---|---|---|
G3群体 | 411871 | 0.3737 | 0.4688 | 0.4973 | 0.5388 |
30号组配 | 411871 | 0.3744 | 0.5081 | 0.5002 | 0.8286 |
31号组配 | 411871 | 0.3031 | 0.4947 | 0.3733 | 0.0000* |
30号组配 | 423886 | 0.3121 | 0.5243 | 0.3879 | 0.0000* |
31号组配 | 423886 | 0.3591 | 0.5291 | 0.4703 | 0.0848 |
注: *表示经过校正后仍显著偏离哈迪-温伯格平衡的位点。 |
Tab. 3 Association analysis of QTL411871 and growth traits in P. fucata using GLM表3 马氏珠母贝 QTL 411871与G3群体生长性状的 GLM 分析 |
因变量 | 平方和 | 自由度 | 均方 | 均方比 | 显著性水平 |
---|---|---|---|---|---|
壳高 | 131.931 | 2 | 65.965 | 1.646 | 0.198 |
壳长 | 190.819 | 2 | 95.409 | 2.662 | 0.075 |
壳宽 | 102.914 | 2 | 51.457 | 2.621 | 0.078 |
铰合线 | 13.046 | 2 | 6.523 | 1.751 | 0.179 |
总重 | 101.711 | 2 | 50.856 | 1.796 | 0.172 |
壳重 | 14.224 | 2 | 7.112 | 0.837 | 0.436 |
软体部重 | 23.358 | 2 | 11.679 | 2.905 | 0.060 |
闭壳肌重 | 1.138 | 2 | 0.569 | 3.853 | 0.032 |
Tab. 4 Multiple comparison of growth traits in P. fucata G3 population at QTL411871 loci表4 QTL411871不同基因型与G3群体生长性状的多重比较 |
基因型 | 数量 | 壳高/mm | 壳长/mm | 壳宽/mm | 铰合线/mm | 总重/g | 壳重/g | 软体部重/g | 闭壳肌重/g |
---|---|---|---|---|---|---|---|---|---|
AT | 45 | 49.09±6.13a | 47.80±6.04a | 17.36±1.88a | 41.22±4.77a | 17.64±5.14a | 9.97±2.61a | 5.47±2.06a | 0.92±0.41a |
AA | 29 | 48.93±7.06a | 46.78±6.48a | 17.70±2.05a | 40.74±4.34a | 17.60±5.92a | 9.85±3.09a | 5.44±2.19a | 0.84±0.42a |
TT | 22 | 46.24±5.68a | 44.21±5.12b | 16.69±1.87a | 38.61±3.76b | 15.17±4.81a | 9.01±3.27b | 4.28±1.59b | 0.64±0.31b |
注: 同一列上标不同小写字母表示差异显著(P<0.05), 下同。 |
Tab. 5 Multiple comparison of growth traits in P. fucata at QTLs loci表5 QTL标记不同基因型在2个组配中的生长性状值的多重比较 |
组配 | QTL | 基因型 | 数量 | 壳高/mm | 壳长/mm | 壳宽/mm | 铰合线/mm | 总重/g | 软体部重/g | 壳重/g |
---|---|---|---|---|---|---|---|---|---|---|
30号 | 411871 | AT | 94 | 29.66±4.11a | 33.14±3.95a | 10.94±3.63a | 30.50±3.63a | 3.92±1.62a | 1.25±0.60a | 2.02±0.78a |
TT | 41 | 29.23±3.20a | 32.60±2.85a | 10.92±1.26a | 30.29±3.15a | 3.76±1.18a | 1.22±0.42a | 1.95±0.53a | ||
AA | 50 | 29.07±3.70a | 32.44±3.43a | 10.67±1.40a | 29.77±3.19a | 3.59±1.21a | 1.16±0.46a | 1.92±0.63a | ||
423886 | AG | 97 | 30.39±4.10a | 33.55±3.96a | 11.03±1.69a | 30.47±3.76a | 4.06±1.67a | 1.31±0.66a | 2.08±0.80a | |
GG | 88 | 28.69±3.13b | 32.45±2.94b | 10.80±1.20a | 30.04±2.90a | 3.64±1.06b | 1.17±0.35a | 1.93±0.53a | ||
31号 | 411871 | AT | 94 | 34.71±3.89a | 37.40±3.41a | 13.21±1.59a | 33.80±3.82a | 5.73±1.78a | 2.00±0.75a | 2.87±0.83a |
TT | 96 | 33.92±3.62a | 36.91±3.60a | 13.01±1.69a | 33.26±3.59a | 5.49±1.65a | 1.81±0.64a | 2.78±0.78a | ||
423886 | AA | 68 | 34.55±4.09a | 37.33±3.79a | 13.10±1.67a | 33.65±3.73a | 5.60±1.85a | 1.94±0.78a | 2.85±0.85a | |
AG | 100 | 34.43±3.53a | 37.29±3.41a | 13.11±1.62a | 33.62±3.84a | 5.72±1.68a | 1.91±0.67a | 2.85±0.80a | ||
GG | 21 | 33.10±3.71a | 35.96±2.96a | 12.85±1.69a | 32.70±3.07a | 5.11±1.47a | 1.74±0.57a | 2.59±0.67a |
注: 同一列上标不同小写字母表示差异显著(P<0.05)。 |
Tab. 6 Multiple comparison of growth traits among diplotypes of QTL in P. fucata表6 QTL标记SNP二倍型生长性状的多重比较 |
组配 | 二倍型 | QTL 411871 | QTL 423886 | 数量 | 壳高/mm | 壳长/mm | 壳宽/mm | 铰合线/mm | 总重/g | 软体部重/g | 壳重/g |
---|---|---|---|---|---|---|---|---|---|---|---|
30号 | C1 | AT | AG | 52 | 30.70±4.33a | 33.88±4.17a | 11.25±1.90a | 30.77±3.90a | 4.28±1.88a | 1.41±0.76a | 2.20±0.92a |
C2 | AA | AG | 25 | 33.42±3.78ab | 33.42±3.27ab | 10.87±1.21a | 30.21±3.16a | 3.83±1.28ab | 1.19±0.46ab | 1.93±0.59ab | |
C3 | TT | AG | 19 | 30.07±3.58ab | 33.88±4.17ab | 11.82±1.55a | 30.29±4.06a | 3.88±1.45ab | 1.20±0.54ab | 2.00±0.96ab | |
C4 | AA | GG | 24 | 29.26±3.36ab | 33.00±2.70ab | 10.97±1.24a | 30.87±2.75a | 3.90±1.03ab | 1.29±0.38ab | 2.07±0.51ab | |
C5 | AT | GG | 42 | 28.60±2.96b | 32.34±2.93b | 10.76±1.14a | 30.31±2.97a | 3.55±0.99b | 1.10±0.33b | 1.90±0.52b | |
C6 | TT | GG | 21 | 28.36±3.26b | 32.11±3.30b | 10.97±1.29a | 30.11±3.06a | 3.57±1.22b | 1.18±0.35b | 1.86±0.56b | |
31号 | D1 | AT | AA | 36 | 35.38±4.44a | 37.89±4.04a | 13.26±1.73a | 34.57±3.76a | 5.89±2.08a | 2.11±0.88a | 2.97±0.97a |
D2 | AT | AG | 44 | 34.49±3.32ab | 37.26±2.99ab | 13.17±1.43a | 33.41±4.05ab | 5.67±1.61ab | 1.93±0.69ab | 2.82±0.76ab | |
D3 | TT | AG | 56 | 34.39±3.72ab | 37.32±3.73ab | 13.16±1.77a | 33.78±3.70ab | 5.77±1.74ab | 1.90±0.66ab | 2.87±0.84ab | |
D4 | AT | GG | 14 | 33.67±4.00ab | 36.56±2.90ab | 13.23±1.78a | 33.03±3.04ab | 5.50±1.52ab | 1.93±0.57ab | 2.74±0.71ab | |
D5 | TT | AA | 32 | 33.62±3.60ab | 36.70±3.57ab | 12.93±1.64a | 32.62±3.51b | 5.27±1.54ab | 1.74±0.63b | 2.72±0.70ab | |
D6 | TT | GG | 7 | 31.98±3.03b | 34.75±2.90b | 12.08±1.30a | 32.03±3.25b | 4.32±1.07b | 1.36±0.34b | 2.29±0.52b |
注: 同一列上标不同小写字母表示差异显著(P<0.05)。 |
The authors have declared that no competing interests exist.
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