马氏珠母贝生长性状QTL鉴定及基因型选择效应分析*

doi:10.11978/2016072

Abstract

Abstract:

QTL411871 was identified in the verifying population of Pinctada fucata, and two genotype matings♀AT×♂AT(Group No.30 ) and ♀TT×♂AT(Group No.31) were established to analyze genotype selection effect of QTL. The results showed that QTL411871 was significantly associated with muscle mass in the verifying population (P<0.05), and AT was the superior genotype. QTL411871 was not significantly associated with growth traits in Groups No. 30 and No. 31 (P>0.05); the genetic effect of AT genotype was small, but it was still the genotype with the maximal average of growth traits. QTL423886 was significantly associated with shell height, shell length and total weight in Group No. 30 (P<0.05), and the oysters with genotype AG were bigger than those with the genotype GG in terms of shell height, shell length and total weight, but it was not significantly associated with growth traits in Group No.31 (P>0.05). Diplotypes were constructed based on the two QTLs, and association analyses revealed that C₁ (ATAG) in Group No.30 and D₁ (ATAA) in Group No.31 might be the most advantageous diplotypes for growth traits. These results are useful for developing molecular marker assisted breeding of Pinctada fucata.

Key words: Pinctada fucata quantitative trait locus (QTL), growth trait, verification, genetic effect

CLC Number:

Q341

Guojian WEI, Yaoguo LI, Jianshi LIN, Maoxian HE. Verification of QTL and genotype selection effect of QTL for growth-related traits in the Pearl Oyster, Pinctada fucata[J].Journal of Tropical Oceanography, 2017, 36(2): 19-25.

Figures/Tables 6

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References 26

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引物名称	序列(5'-3')	退火温度/℃	等位基因	对应产物/bp
FI	GGAAACGGAATTCACATTCATTATCTTTA	64	A	200
RI	AATGTCAAGCTGGATATAATTTAGCTCTA	60	T	171
FO	AAATTATTGAAACTATTTCACCGATTCG	62	空白	空白
RO	TTGTGATTTCATTTTCGTATCAATATTCTT	62	空白	空白

群体/组配	QTL	PIC	H_o	H_e	HWE P值
G3群体	411871	0.3737	0.4688	0.4973	0.5388
30号组配	411871	0.3744	0.5081	0.5002	0.8286
31号组配	411871	0.3031	0.4947	0.3733	0.0000*
30号组配	423886	0.3121	0.5243	0.3879	0.0000*
31号组配	423886	0.3591	0.5291	0.4703	0.0848

因变量	平方和	自由度	均方	均方比	显著性水平
壳高	131.931	2	65.965	1.646	0.198
壳长	190.819	2	95.409	2.662	0.075
壳宽	102.914	2	51.457	2.621	0.078
铰合线	13.046	2	6.523	1.751	0.179
总重	101.711	2	50.856	1.796	0.172
壳重	14.224	2	7.112	0.837	0.436
软体部重	23.358	2	11.679	2.905	0.060
闭壳肌重	1.138	2	0.569	3.853	0.032

基因型	数量	壳高/mm	壳长/mm	壳宽/mm	铰合线/mm	总重/g	壳重/g	软体部重/g	闭壳肌重/g
AT	45	49.09±6.13^a	47.80±6.04^a	17.36±1.88^a	41.22±4.77^a	17.64±5.14^a	9.97±2.61^a	5.47±2.06^a	0.92±0.41^a
AA	29	48.93±7.06^a	46.78±6.48^a	17.70±2.05^a	40.74±4.34^a	17.60±5.92^a	9.85±3.09^a	5.44±2.19^a	0.84±0.42^a
TT	22	46.24±5.68^a	44.21±5.12^b	16.69±1.87^a	38.61±3.76^b	15.17±4.81^a	9.01±3.27^b	4.28±1.59^b	0.64±0.31^b

组配	QTL	基因型	数量	壳高/mm	壳长/mm	壳宽/mm	铰合线/mm	总重/g	软体部重/g	壳重/g
30号	411871	AT	94	29.66±4.11^a	33.14±3.95^a	10.94±3.63^a	30.50±3.63^a	3.92±1.62^a	1.25±0.60^a	2.02±0.78^a
		TT	41	29.23±3.20^a	32.60±2.85^a	10.92±1.26^a	30.29±3.15^a	3.76±1.18^a	1.22±0.42^a	1.95±0.53^a
		AA	50	29.07±3.70^a	32.44±3.43^a	10.67±1.40^a	29.77±3.19^a	3.59±1.21^a	1.16±0.46^a	1.92±0.63^a
	423886	AG	97	30.39±4.10^a	33.55±3.96^a	11.03±1.69^a	30.47±3.76^a	4.06±1.67^a	1.31±0.66^a	2.08±0.80^a
	423886	GG	88	28.69±3.13^b	32.45±2.94^b	10.80±1.20^a	30.04±2.90^a	3.64±1.06^b	1.17±0.35^a	1.93±0.53^a
31号	411871	AT	94	34.71±3.89^a	37.40±3.41^a	13.21±1.59^a	33.80±3.82^a	5.73±1.78^a	2.00±0.75^a	2.87±0.83^a
	411871	TT	96	33.92±3.62^a	36.91±3.60^a	13.01±1.69^a	33.26±3.59^a	5.49±1.65^a	1.81±0.64^a	2.78±0.78^a
	423886	AA	68	34.55±4.09^a	37.33±3.79^a	13.10±1.67^a	33.65±3.73^a	5.60±1.85^a	1.94±0.78^a	2.85±0.85^a
		AG	100	34.43±3.53^a	37.29±3.41^a	13.11±1.62^a	33.62±3.84^a	5.72±1.68^a	1.91±0.67^a	2.85±0.80^a
		GG	21	33.10±3.71^a	35.96±2.96^a	12.85±1.69^a	32.70±3.07^a	5.11±1.47^a	1.74±0.57^a	2.59±0.67^a

Verification of QTL and genotype selection effect of QTL for growth-related traits in the Pearl Oyster, Pinctada fucata

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组配	二倍型	QTL 411871	QTL 423886	数量	壳高/mm	壳长/mm	壳宽/mm	铰合线/mm	总重/g	软体部重/g	壳重/g
30号	C₁	AT	AG	52	30.70±4.33^a	33.88±4.17^a	11.25±1.90^a	30.77±3.90a	4.28±1.88^a	1.41±0.76a	2.20±0.92^a
	C₂	AA	AG	25	33.42±3.78^ab	33.42±3.27^ab	10.87±1.21^a	30.21±3.16^a	3.83±1.28^ab	1.19±0.46^ab	1.93±0.59^ab
	C₃	TT	AG	19	30.07±3.58^ab	33.88±4.17^ab	11.82±1.55^a	30.29±4.06^a	3.88±1.45^ab	1.20±0.54^ab	2.00±0.96^ab
	C₄	AA	GG	24	29.26±3.36^ab	33.00±2.70^ab	10.97±1.24^a	30.87±2.75^a	3.90±1.03^ab	1.29±0.38^ab	2.07±0.51^ab
	C₅	AT	GG	42	28.60±2.96^b	32.34±2.93^b	10.76±1.14^a	30.31±2.97^a	3.55±0.99^b	1.10±0.33^b	1.90±0.52^b
	C₆	TT	GG	21	28.36±3.26^b	32.11±3.30^b	10.97±1.29^a	30.11±3.06^a	3.57±1.22^b	1.18±0.35^b	1.86±0.56^b
31号	D₁	AT	AA	36	35.38±4.44^a	37.89±4.04^a	13.26±1.73^a	34.57±3.76^a	5.89±2.08^a	2.11±0.88^a	2.97±0.97^a
	D₂	AT	AG	44	34.49±3.32^ab	37.26±2.99^ab	13.17±1.43^a	33.41±4.05^ab	5.67±1.61^ab	1.93±0.69^ab	2.82±0.76^ab
	D₃	TT	AG	56	34.39±3.72^ab	37.32±3.73^ab	13.16±1.77^a	33.78±3.70^ab	5.77±1.74^ab	1.90±0.66^ab	2.87±0.84^ab
	D₄	AT	GG	14	33.67±4.00^ab	36.56±2.90^ab	13.23±1.78^a	33.03±3.04^ab	5.50±1.52^ab	1.93±0.57^ab	2.74±0.71^ab
	D₅	TT	AA	32	33.62±3.60^ab	36.70±3.57^ab	12.93±1.64^a	32.62±3.51^b	5.27±1.54^ab	1.74±0.63^b	2.72±0.70^ab
	D₆	TT	GG	7	31.98±3.03^b	34.75±2.90^b	12.08±1.30^a	32.03±3.25^b	4.32±1.07^b	1.36±0.34^b	2.29±0.52^b

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