检索
E-mail
RSS

作者投稿 主编审稿 专家审稿 远程编辑

热带海洋学报 ›› 2012, Vol. 31 ›› Issue (4): 117-123.doi: 10.11978/j.issn.1009-5470.2012.04.016 cstr: 32234.14.j.issn.1009-5470.2012.04.016

• 海洋生物学 • 上一篇    下一篇

氮、磷对克氏前沟藻三亚株生长及产毒特性的影响

龙超1,2, 龙丽娟1   

  1. 1.中国科学院南海海洋研究所, 中国科学院海洋生物资源可持续利用重点实验室, 广东省海洋药物重点实验室, 广东 广州 510301; 
    2.中国科学院研究生院, 北京 100049
  • 收稿日期:2011-02-19 修回日期:2011-04-17 出版日期:2012-10-22 发布日期:2012-10-24
  • 通讯作者: 龙丽娟。E-mail: longlj@scsio.ac.cn
  • 作者简介:龙超(1984—), 男, 湖北省黄冈市人, 硕士研究生, 研究方向为海洋生物学。E-mail: Clongflying@163.com
  • 基金资助:

    中国科学院重要方向项目(KSCX2-YW-R-093); 海洋公益性行业科研专项 (200705026)

Effects of nitrate and phosphate on the growth and toxin production of Amphidinium klebsii (Sanya strain)

LONG Chao1,2, LONG Li-juan1   

  1. 1. Guangdong Key Laboratory of Marine Material, Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; 
    2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2011-02-19 Revised:2011-04-17 Online:2012-10-22 Published:2012-10-24

PDF (PC)

赞

被引次数

7 | 1

摘要：

以改良K培养液为基础, 通过设定不同的N、P浓度水平, 探讨N、P营养盐对克氏前沟藻Amphidinium klebsii(三亚株)生长及产毒特性的影响。研究结果表明, N、P均为影响克氏前沟藻生长和产毒的重要因素。低浓度的N、P(N浓度≤4.665×10-4mol•L-1, P浓度≤2.000×10-5mol•L-1)不利于克氏前沟藻生物量的积累, 但能促进其生长及毒素的产生; 中浓度的N、P(4.665×10-4mol•L-1-1, 2.000×10-5mol•L-1-5 mol•L-1)有利于克氏前沟藻生物量的积累, 却不利于毒素的产生; N、P浓度过高(N浓度≥37.320×10-4mol•L-1, P浓度≥16.000×10-5mol•L-1)则会抑制克氏前沟藻的生长, 但能促进毒素的产生, 并有相对较高的细胞终密度。克氏前沟藻生长的最佳N、P浓度范围分别为4.665×10-4—37.320×10-4mol•L-1和2.000×10-5 —16.000×10-5mol•L-1。当N浓度为9.330×10?4mol•L-1, P浓度为4.000×10-5mol•L-1时, 克氏前沟藻达到最高密度1.237×105mL-1, 单位体积活血活性达到最高值347.48HU•L-1。

关键词: 克氏前沟藻, 溶血毒素, 氮, 磷

Abstract:

In order to evaluate the growth and toxin production of Amphidinium klebsii (Sanya strain) under different nitrate and phosphate concentrations, a series of nitrate and phosphate solutions were prepared based on modified K medium. Nitrate and phosphate had significant effects on the growth and toxin productivity of A. klebsii. Low-nitrate and low-phosphate concentrations (N concentration≤4.665×10-4mol•L-1, P concentration≤2.000×10-5mol•L-1) in culture media were not favorable to the biomass accumulation of A. klebsii but favorable to its growth and toxin production. On the contrary, middle-nitrate and middle-phosphate concentrations (4.665×10-4mol•L-1-4mol•L-1, 2.000×10-5mol•L-1-5mol•L-1) were favorable to the biomass accumulation of A. klebsii but not favorable to its toxin production. Extra-high-level nitrate and phosphate concentrations (N concentration≥37.320×10-4mol•L-1, P concentration≥16.000×10-5mol•L-1) seemed to inhibit the growth of A. klebsii, but resulted in higher cell densities and contributed to its toxin production. Under the experimental condition, optimum growth N and P concentrations were 4.665×10-4mol•L-1-4mol•L-1, 2.000×10-5mol•L-1-5mol•L-1. The highest biomass value estimated in culture was 1.237×105mL-1 at the levels of nitrate 9.330×10–4mol•L–1 and phosphate 4.000×10–5mol•L-1, and the maximum hemolytic activity reached 347.48HU•L-1 under the same condition.

Key words: Amphidinium klebsii, haemolytic toxin, nitrate, phosphate

中图分类号: 

  • Q178.53