北部湾棕囊藻藻华原因种分析

doi:10.11978/2020030

Abstract

Abstract:

In recent years, Phaeocystis has become the main causative species for algal blooms in the Beibu Gulf of China. It has deleterious effects on marine ecosystem and aquaculture, and causes nuclear power plant safety problems in Fangcheng. It is thus urgent to further analyze the causative species of Phaeocystis in the region. In February and March 2017, nine strains of Phaeocystis sp. identified by optical microscope were isolated during algal blooms in Qinzhou Bay and Weizhou Island; their SSU rDNA sequences were then analyzed using Maximum Likelihood (ML) phylogenetic tree. The results showed that all nine strains of Phaeocystis sp. were morphologically consistent with those of Phaeocystis globosa. Their genetic distance varied between 0.0000-0.0073; and in the phylogenetic tree, all clustered in the same branch with those P. globosa from other seawaters. Among them, WZS3-1 strain in the Beibu Gulf had the closest genetic relationship with P. globosa from Gulf of Mexico CCMP627, Surinam CCMP628, Ecuador CCMP1528, South Africa P162, China Sea RCC:K1398 and Santou97, exhibiting a genetic distance of 0.0000. Strains of WZS1, WZS2, WZS4, and WZS5 in the Beibu Gulf had a genetic distance of 0.0000 with Hong Kong SKLMP_T005 and Bohai BOHAI1. The intraspecific genetic distance of P. globosa (0.0000-0.0071) is significantly smaller than the interspecific genetic distance of P. globosa (0.0084-0.0440). As a result, these nine strains from Beibu Gulf all belong to P. globosa. The phylogenetic distance for strains of P. globosa do not reflect their geographical distance. The findings of this study are essential for Phaeocystis culture collection, their further ecophysiological study and understanding of occurrence mechanisms for Phaeocystis blooms in the Beibu Gulf.

Key words: Phaeocystis globosa, phylogenetic tree, genetic distance, harmful algal blooms, Beibu Gulf

CLC Number:

XU Yixiao, HE Xilin, ZHANG Teng, LAN Wenlu. Causative species of Phaeocystis blooms in Beibu Gulf[J].Journal of Tropical Oceanography, 2020, 39(6): 122-130.

Figures/Tables 4

Tab. 1

Tab. 2

Fig. 1

Fig. 2

References 31

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株名	采集地	采集时间
QJ-1	钦州湾(21°41′38″N, 108°35′13″E)	2017-02-22
QJ-2	钦州湾(21°41′38″N, 108°35′13″E)	2017-02-22
QJ-3	钦州湾(21°41′38″N, 108°35′13″E)	2017-02-22
WZS1	涠洲岛(21°03′16″N, 109°08′71″E)	2017-03-21
WZS2	涠洲岛(21°06′44″N, 109°10′36″E)	2017-03-21
WZS3-1	涠洲岛(21°06′48″N, 109°13′43″E)	2017-03-21
WZS3-2	涠洲岛(21°06′48″N, 109°13′43″E)	2017-03-21
WZS4	涠洲岛(21°03′23″N, 109°13′15″E)	2017-03-21
WZS5	涠洲岛(21°02′42″N, 109°11′13″E)	2017-03-21

种	株系	来源地	登录号
P. globosa	QJ-1	中国, 钦州湾	MN826692
P. globosa	QJ-2	中国, 钦州湾	MN826693
P. globosa	QJ-3	中国, 钦州湾	MN826694
P. globosa	WZS1	中国, 涠洲岛	MN826695
P. globosa	WZS2	中国, 涠洲岛	MN826696
P. globosa	WZS3-1	中国, 涠洲岛	MN826697
P. globosa	WZS3-2	中国, 涠洲岛	MN826698
P. globosa	WZS4	中国, 涠洲岛	MN826699
P. globosa	WZS5	中国, 涠洲岛	MN826670
P. globosa	-	中国香港	DQ075202
P. globosa	SKLMP_T005	中国香港	MG914020
P. globosa	BOHAI1	中国, 渤海	EU077556
P. globosa	-	中国, 渤海	EU024765
P. globosa	RCC:K1398	太平洋-中国海域	JX660987
P. globosa	Santou97	中国, 汕头	AJ279499
P. globosa	CCMP628	大西洋, 苏里南	AF182112
P. globosa	W07.002.01	英国	GQ118979
P. globosa	robertsonii	南大西洋湾	EF100712
P. globosa	CCMP627	墨西哥湾	AF182111
P. globosa	SK35	德国湾	X77476
P. globosa	PLY147	比利时	AY851300
种	株系	来源地	登录号
P. globosa	CCMP1528	厄瓜多尔	AF182110
P. globosa	P162	南非	AF182113
P. globosa	CCMP1524	泰国	AF182109
P. globosa	RCC:PCC64	英吉利海峡	JX660994
P. globosa	RCC:RCC678	英国, 北海(大西洋)	JX660989
P. globosa	RCC:NIES388	日本	JX660988
P. globosa	PCC540	东大西洋	AF182115
P. globosa	RCC:RCC1737	太平洋	JX660993
P. pouchetii	P360	挪威, 劳内峡湾	AF182114
P. pouchetii	SK34	格陵兰岛	X77475
P. antarctica	SK22	南大洋威德尔海	X77480
P. antarctica	MM-E1B6	-	JN381493
P. jahnii	NIES-3884	地中海	LC189148
P. jahnii	CCMP2496	地中海	AF163148
P. cordata	-	地中海	AF163147
Emiliania huxleyi	Texel B	-	AF184167

Causative species of Phaeocystis blooms in Beibu Gulf

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