广州市南沙水产养殖区抗生素的残留特性*

doi:10.11978/2016001

Abstract

Abstract:

The residual antibiotics have caused public concerns with the rapid development of aquaculture, due to their adverse effects on aquatic ecosystems and human health. A method named solid-phase extraction-rapid resolution liquid chromatography-tandem mass spectrometry (SPE-RRLC-MS/MS) was used to determine the occurrence and bioaccumulation of 35 antibiotics (14 sulfonamides, 10 fluoroquinolones, 6 macrolides, 2 tetracyclines and 3 others) in environmental matrix and different tissues of fish in the Nansha aquaculture area of Guangzhou. Twelve antibiotics were detected in water, suspended particulates, sediments and feeds, with maximum concentrations of 60.76 µg·L^-1(Erythromycin-H₂O), 1691 ng·g^-1(Monensin), 2.41 ng·g^-1(Enrofloxacin) and 74.96 ng·g^-1 (Enrofloxacin), respectively. Eleven antibiotics were detected in fish plasma, livers and muscle, with maximum concentrations of 33.52 µg·L^-1(Erythromycin-H₂O), 199 ng·g^-1(Norfloxacin) and 22.30 ng·g^-1(Norfloxacin), respectively. The values of bioaccumulation factors (BAFs) for the detected antibiotics in fish plasma, livers, and muscle were in the ranges of 147~336, 7~8891 and 8~16, respectively, indicating a different bioaccumulation capacity for different tissues. Human health risk assessment based on potential fish consumption indicated that residues of antibiotics in these aquatic products do not pose risks to consumers’ health. The current study is of great significance in ensuring the safety of aquatic products in Guangzhou, promoting the sustainable development of aquaculture and improving the regulation on residual antibiotics for environmental protection agencies.

Key words: aquaculture, antibiotics, fish tissues, bioaccumulation, health risk

Qinwei HAO, Xiangrong XU, Hui CHEN, Shan LIU, Jun CHEN, Shuangshuang LIU, Guangguo YING. Residual antibiotics in the Nansha aquaculture area of Guangzhou[J].Journal of Tropical Oceanography, 2017, 36(1): 106-113.

Figures/Tables 6

Fig. 1

Tab. 1

Tab. 2

Tab. 3

Tab. 4

Tab. 5

References 31

[1]	高盼盼, 罗义, 周启星, 等, 2009. 水产养殖环境中抗生素抗性基因(ARGs)的研究及进展[J]. 生态毒理学报, 4(6): 770-779.
	GAO PANPAN, LUO YI, ZHOU QIXING, et al, 2009. Research advancement of antibiotics resistance genes (ARGs) in Aquaculture Environment[J]. Asian Journal of Ecotoxicology, 4(6): 770-779 (in Chinese with English abstract).
[2]	广州市统计局, 2015[2016-01-05]. 广州统计信息手册[EB/OL]. 广州: 广州市统计局. .
[3]	何秀婷, 王奇, 聂湘平, 等, 2014. 广东典型海水养殖区沉积物及鱼体中磺胺类药物的残留及其对人体的健康风险评价[J]. 环境科学, 35(7): 2728-2735.
	HE XIUTING, WANG QI, NIE XIANGPING, et al, 2014. Residues and health risk assessment of sulfonamides in sediment and fish from typical marine aquaculture regions of Guangdong Province, China[J]. Environmental Science, 35(7): 2728-2735 (in Chinese with English abstract).
[4]	梁惜梅, 施震, 黄小平, 2013. 珠江口典型水产养殖区抗生素的污染特征[J]. 生态环境学报, 22(2): 304-310.
	LIANG XIMEI, SHI ZHEN, HUANG XIAOPING, 2013. Occurrence of antibiotics in typical aquaculture of the Pearl River Estuary[J]. Ecology and Environmental Sciences, 22(2): 304-310 (in Chinese with English abstract).
[5]	任珂君, 刘玉, 徐健荣, 等, 2015. 广东一饮用水源地河流沉积物及鱼体中氟喹诺酮类(FQs)抗生素残留特征研究[J]. 环境科学学报, 36(3): 760-766.
	REN KEJUN, LIU YU, XU JIANRONG, et al, 2015. Residues characteristics of fluoroquinolones (FQs) in the river sediments and fish tissues in a drinking water protection area of Guangdong Province[J]. Acta Scientiae Circumstantiae, 36(3): 760-766 (in Chinese with English abstract).
[6]	王群, 刘淇, 唐雪莲, 等, 2005. 诺氟沙星在养殖鲈体内的代谢动力学和残留研究[J]. 水产学报, 28(S): 13-18.
	WANG QUN, LIU QI, TANG XUELIAN, et al, 2005. Pharmacokinetics and residues of norfloxacin in Lateolabras japonicus[J]. Journal of Fisheries of China, 28(S): 13-18 (in Chinese with English abstract).
[7]	中华人民共和国农业部, 2002(2010-07-16) [2016-01-05]. 中华人民共和国农业部公告第235号公告: 动物性食品中兽药最高残留限量[EB/OL]. 北京: 中华人民共和国农业部, .
[8]	ADACHI F, YAMAMOTO A, TAKAKURA K-I, et al, 2013. Occurrence of fluoroquinolones and fluoroquinolone- resistance genes in the aquatic environment[J]. Science of the Total Environment, 444: 508-514.
[9]	Australian Government, Department of Health, Office of Chemical Safety (OCS), [2016-01-05]>. Acceptable daily intakes for agricultural and veterinary chemicals: current as of 31 March 2016[EB/OL]. .
[10]	BARROS-BECKER F, ROMERO J, PULGAR A, et al, 2012. Persistent oxytetracycline exposure induces an inflammatory process that improves regenerative capacity in zebrafish larvae[J]. PLoS One, 7(5): e36827.
[11]	BOONSANER M, HAWKER D W, 2013. Evaluation of food chain transfer of the antibiotic oxytetracycline and human risk assessment[J]. Chemosphere, 93(6): 1009-1014.
[12]	CHEN HUI, LIU SHAN, XU XIANGRONG, et al, 2015. Antibiotics in typical marine aquaculture farms surrounding Hailing Island, South China: Occurrence, bioaccumulation and human dietary exposure[J]. Marine Pollution Bulletin, 90(1-2): 181-187.
[13]	DONE H Y, HALDEN R U, 2015. Reconnaissance of 47 antibiotics and associated microbial risks in seafood sold in the United States[J]. Journal of Hazardous Materials, 282: 10-17.
[14]	DUNNIVANT F M, ANDERS E, 2006. A basic introduction to pollutant fate and transport: an integrated approach with chemistry, modeling, risk assessment, and environmental legislation[M]. New York: John Wiley & Sons.
[15]	FAO/WHO, 2011[2016-01-05]. Residues of some veterinary drugs in animals and foods[R/OL]. Geneva: FAO/WHO. https://openlibrary.org/works/OL749344W/Residues_of_some_veterinary_drugs_in_animals_ and_foods.
[16]	FAO/WHO, 2014. [2016-01-05]. Evaluations of the joint FAO/WHO expert committee on food additives (JECFA) [R/OL]. Geneva: FAO/WHO. .
[17]	FSCJ (Food Safety Commission of Japan), 2007. [2016-01-05]. Veterinary medicinal products[EB/OL]. .
[18]	GAO LIHONG, SHI YALI, LI WENHUI, et al, 2012. Occurrence, distribution and bioaccumulation of antibiotics in the Haihe River in China[J]. Journal of Environmental Monitoring, 14(4): 1247-1254.
[19]	JFCRF (The Japan Food Chemical Research Foundation), 2016. [2016-01-05]. Maximum residue limits (MRLs) list of agricultural chemicals in foods: Positive list system for agricultural chemical residues in foods[EB/OL]. .
[20]	RAMIREZ A J, MOTTALEB M A, BROOKS B W, et al, 2007. Analysis of pharmaceuticals in fish using liquid chromatography-tandem mass spectrometry[J]. Analytical Chemistry, 79(8): 3155-3163.
[21]	RICO A, OLIVEIRA R, MCDONOUGH S, et al, 2014. Use, fate and ecological risks of antibiotics applied in tilapia cage farming in Thailand[J]. Environmental Pollution, 191: 8-16.
[22]	SASSMAN S A, LEE L S, 2005. Sorption of three tetracyclines by several soils: assessing the role of pH and cation exchange[J]. Environmental Science & Technology, 39(19): 7452-7459.
[23]	STADNICKA J, SCHIRMER K, ASHAUER R, 2012. Predicting concentrations of organic chemicals in fish by using toxicokinetic models[J]. Environmental Science & Technology, 46(6): 3273-3280.
[24]	SU HAOCHANG, YING GUANGGUO, TAO RAN, et al, 2012. Class 1 and 2 integrons, sul resistance genes and antibiotic resistance in Escherichia coli isolated from Dongjiang River, South China[J]. Environmental Pollution, 169: 42-49.
[25]	TOFTEN H, JOBLING M, 1996. Development of spinal deformities in Atlantic salmon and Arctic charr fed diets supplemented with oxytetracycline[J]. Journal of Fish Biology, 49(4): 668-677.
[26]	WHO, 1997. [2016-01-05]. Toxicological evaluation of certain veterinary drug residues in food[R/OL]. Geneva: WHO. .
[27]	XU WEI-HAI, ZHANG GAN, ZOU SHI-CHUN, et al, 2007. Determination of selected antibiotics in the Victoria Harbour and the Pearl River, South China using high-performance liquid chromatography-electrospray ionization tandem mass spectrometry[J]. Environmental Pollution, 145(3): 672-679.
[28]	ZHANG RUIJIE, ZHANG GAN, ZHENG QIAN, et al, 2012. Occurrence and risks of antibiotics in the Laizhou Bay, China: impacts of river discharge[J]. Ecotoxicology and Environmental Safety, 80: 208-215.
[29]	ZHAO JIANLIANG, LIU YOUSHENG, LIU WANGRONG, et al, 2015. Tissue-specific bioaccumulation of human and veterinary antibiotics in bile, plasma, liver and muscle tissues of wild fish from a highly urbanized region[J]. Environmental Pollution, 198: 15-24.
[30]	ZHOU LIJUN, YING GUANGGUO, LIU SHAN, et al, 2012. Simultaneous determination of human and veterinary antibiotics in various environmental matrices by rapid resolution liquid chromatography-electrospray ionization tandem mass spectrometry[J]. Journal of Chromatography A, 1244: 123-138.
[31]	ZOU SHICHUN, XU WEIHAI, ZHANG RUIJIE, et al, 2011. Occurrence and distribution of antibiotics in coastal water of the Bohai Bay, China: impacts of river discharge and aquaculture activities[J]. Environmental Pollution, 159(10): 2913-2920.

化合物	S1				S2
	水体残留量/ (ng·L^-1)	颗粒物残留量/(ng·g^-1)	沉积物残留量/(ng·g^-1)	饲料残留量/(ng·g^-1)	水体残留量/(ng·L^-1)	颗粒物残留量/(ng·g^-1)	沉积物残留量/(ng·g^-1)	饲料残留量/(ng·g^-1)
	脱水红霉素	60.76	<0.26	<0.26	5.22	17.25	<0.26	<0.26	43.68
北里霉素	N.D.	N.D.	N.D.	0.5	N.D.	2.57	N.D.	N.D.
诺氟沙星	N.D.	110	1.66	15.21	N.D.	94.39	1.71	N.D.
环丙沙星	N.D.	N.D.	N.D.	14.76	N.D.	N.D.	N.D.	N.D.
恩诺沙星	N.D.	92.24	2.41	74.96	N.D.	47.91	N.D.	N.D.
氧氟沙星	N.D.	N.D.	1.54	15.89	N.D.	N.D.	N.D.	N.D.
磺胺嘧啶	0.19	0.65	0.02	0.50	0.62	0.17	N.D.	0.11
甲氧苄啶	0.39	N.D.	N.D.	16.99	0.11	N.D.	N.D.	N.D.
磺胺喹恶啉	N.D.	N.D.	N.D.	1.77	N.D.	N.D.	N.D.	N.D.
磺胺二甲嘧啶	N.D.	N.D.	N.D.	3.26	N.D.	N.D.	N.D.	N.D.
莫能菌素	N.D.	N.D.	N.D.	0.36	0.15	1691	N.D.	0.14
林可霉素	N.D.	N.D.	<2.95	N.D.	N.D.	N.D.	N.D.	N.D.

化合物	血液		肝脏		肌肉
化合物	检出率	均值(范围)/(µg·L^-1)	检出率	均值(范围)/(ng·g^-1)	检出率	均值(范围)/(ng·g^-1)
脱水红霉素	100%	11.02 (2.46~33.52)	100%	<3.81 (0~3.81)	100%	<0.78 (0~0.78)
诺氟沙星	100%	11.96 (9.20~24.58)	77%	31.79 (N.D.~199)	100%	<3.77 (1.95~22.30)
培氟沙星	N.D.		N.D.		15%	0.25 (N.D. ~1.66)
氟罗沙星	N.D.		N.D.		8%	0.08 (N.D.~1.07)
环丙沙星	N.D.		N.D.		N.D.
恩诺沙星	N.D.		N.D.		92%	2.19 (N.D.~3.13)
氧氟沙星	N.D.		N.D.		54%	0.72 (N.D.~1.40)
磺胺嘧啶	100%	<0.36 (0.36~0.38)	23%	<0.15 (N.D.~0.28)	38%	<0.05 (N.D.~0.12)
甲氧苄啶	N.D.		N.D.		8%	0.02 (N.D.~0.22)
莫能菌素	N.D.		46%	1.70 (N.D.~8.20)	N.D.
林可霉素	N.D.		N.D.		N.D.

化合物	血液		肝脏		肌肉
化合物	检出率	均值(范围)/(µg·L^-1)	检出率	均值(范围)/ (ng·g^-1)	检出率	均值(范围)/(ng·g^-1)
脱水红霉素	100%	12.09 (9.17~19.95)	100%	<3.81 (0~3.81)	100%	<0.78 (0~0.78)
诺氟沙星	100%	8.81 (5.08~10.10)	100%	46.92 (1.91~163)	100%	2.59 (2.05~4.07)
恩诺沙星	N.D.		N.D.		43%	0.77 (N.D.~2.05)
氧氟沙星	N.D.		N.D.		28%	0.36 (N.D.~1.36)
磺胺嘧啶	100%	<0.36 (0~0.36)	14%	<0.09 (N.D.~0.64)	71%	<0.09 (N.D.~0.12)
莫能菌素	N.D.		43%	4.00 (N.D.~10.93)	N.D.
林可霉素	N.D.		N.D.		N.D.

组织	化合物	BAF
血液	脱水红霉素	324(40~974)
血液	磺胺嘧啶	154(0~1984)
肝脏	磺胺嘧啶	7(0~144)
肝脏	莫能菌素	9335(0~72900)
肌肉	磺胺嘧啶	17(0~156)
肌肉	甲氧苄啶	8(0~160)

化合物	C_fish/(μg·kg^-1)	摄入量/(mg·kg^-1·d^-1)	ADI/(mg·kg^-1 bw)	HQ	MRL/(μg·kg^-1)
磺胺嘧啶	0.12	8.9×10^-9	0.02 (Australian Government et al, 2016)	4.5×10^-7	100 (中华人民共和国农业部, 2002)
诺氟沙星	22.30	1.7×10^-5	0.0114 (FAO/WHO, 2011)	1.5×10^-3	100 (JFCRF, 2016)
氧氟沙星	1.40	1.0×10^-6	0.0032 (FSCJ, 2007)	3.2×10^-4	50 (JFCRF, 2016)
恩诺沙星	3.13	2.3×10^-6	0.0006 (WHO, 1997)	3.9×10^-3	100 (中华人民共和国农业部, 2002)
脱水红霉素	0.78	5.8×10^-7	0.0007 (FAO/WHO, 2014)	8.2×10^-4	200 (中华人民共和国农业部, 2002)

Residual antibiotics in the Nansha aquaculture area of Guangzhou

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 31

Related Articles 12

Metrics

Comments

Recommended 0

[1]	WU Huiyu, GAO Xiuju, GE Dongzhen, ZHU Jun, ZOU Xiaoxiao, HUANG Huiqin, GU Zhifeng, BAO Shixiang. Screening and identification of high protease producing strain and its application in culture of Babylonia areolata [J]. Journal of Tropical Oceanography, 2023, 42(5): 124-133.
[2]	WANG Chenyan, SHI Jingwen, YAN Annan, KANG Yaru, WANG Yuxuan, QIN Suli, HAN Minwei, ZHANG Ruijie, YU Kefu. Bioaccumulation characteristics and source apportionment of organophosphate esters in Acanthaster planci from the South China Sea [J]. Journal of Tropical Oceanography, 2023, 42(5): 30-37.
[3]	LI Bing, WANG Ruixuan, ZHANG Li, LUO Bang, MOU Hongli, WANG Jiangyong. The number and antibiotic resistance of heterotrophic bacteria in Crassostrea hongkongensis in aquaculture areas of Beibu Gulf [J]. Journal of Tropical Oceanography, 2021, 40(4): 70-83.
[4]	PAN Cuihong, XIA Lihua, WU Zhifeng, WANG Meng, XIE Xuetong, WANG Fang. Remote sensing retrieval of chlorophyll-a concentration in coastal aquaculture area of Zhelin Bay [J]. Journal of Tropical Oceanography, 2021, 40(1): 142-153.
[5]	HAO Qing, SUN Yu-xin, XU Xiang-rong, LUO Xiao-jun, WANG Shuai-long, ZHANG Zai-wang, MAI Bi-xian. Residual levels of PCBs and DDTs in wholesale fish in Guangzhou, China and potential health risks [J]. Journal of Tropical Oceanography, 2014, 33(5): 84-91.
[6]	CHENG Xiang-ju, GUO Zhen-ren, LIU Guo, LI Bin. Study on flux of nitrogen and phosphorus across the interface of sediment-water and their diffusion areas in seawater at the aquaculture region of Daya Bay [J]. Journal of Tropical Oceanography, 2014, 33(4): 77-84.
[7]	ZHOU Qin, WANG Yu-fen, JIANG Xiu-feng. Giant clam research and prospect of aquaculture in the South China Sea [J]. Journal of Tropical Oceanography, 2014, 33(2): 87-93.
[8]	LI Lei, WANG Yun-long, SHEN Ang-lü, JIANG Mei, HUANG Hou-jian, SHEN Xin-qiang. Kinetic study on the bioconcentration of heavy metals Cu and Pb in Meretrix meretrix under the condition of sediment exposure [J]. Journal of Tropical Oceanography, 2013, 32(1): 70-75.
[9]	ZHU Chun-hua,SHEN Yu-chun,XIE En-yi,YE Ning,WANG Yan,DU Xiao-dong,WU Zao-h. Aquaculture carrying capacity of Pinctada martensii in Liusha Bay of Zhanjiang [J]. Journal of Tropical Oceanography, 2011, 30(3): 76-81.
[10]	MENG Fan-ping,YU Teng. Review on bioaccumulation and toxicity of polybrominated diphenyl ethers in marine organisms [J]. Journal of Tropical Oceanography, 2010, 29(5): 1-9.
[11]	GAO Lei,YANG Xiao-mei,SU Fen-zhen,LIU Yong,. Remote sensing analysis of gravity-center migration of the aquaculture in the Zhujiang River Estuary [J]. Journal of Tropical Oceanography, 2010, 29(3): 35-40.
[12]	ZHANG Qiao-min,SHI Qi,YU Ke-fu. Review of eco-development model for mangrove land-based enclosure aquaculture in the Pearl River Estuary [J]. Journal of Tropical Oceanography, 2010, 29(1): 8-14.