九孔鲍MSTN基因cSNP多态性及与生长性状关联分析

doi:10.11978/2018053

Abstract

Abstract:

Myostatin is an important member of the transforming growth factor (TGF) family that functions to regulate muscle development and growth in animals. It has become one of the most important target genes for genetic improvement of the production of farmed animals. The purpose of this study was to analyze correlation between single nucleotide polymorphisms of the myostatin gene and growth traits of the Haliotis diversicolor supertexta, which is an important aquaculture shellfish. The single nucleotide polymorphisms of the myostatin gene (Hs-MSTN) of H. diversicolor supertexta were identified by direct sequencing of PCR products, and association analysis of SNPs with H. diversicolor supertexta growth traits and Hs-MSTN expression level. Nine SNPs were identified in cDNA of Hs-MSTN, including four synonymous mutations. The associations of these SNPs were analyzed with H. diversicolor supertexta growth traits, including shell length, shell height, and body weight. The g909C>T SNP locus was found to be associated with shell length, shell height, and body weight. The TT type H. diversicolor supertexta showed significantly higher traits values than those of TC and CC types in body weight, and the TT type H. diversicolor supertexta also showed significantly higher traits values than CC type in shell length and shell height (p<0.05). On the contrary, Hs-MSTN transcripts in the musle of those of TC and CC types significantly more than in that of TT type (p<0.05). The results suggested that Hs-MSTN might regulate the addutor musle growth negatively, and SNP g909C>T could be used as a candidate marker for slective breeding of H. diversicolor supertexta.

Key words: Haliotis diversicolor supertexta, MSTN, SNP, growth traits, association analysis

CLC Number:

P735.542

Jialin AI, Zhimin LI, Jianyong LIU, Yuchun SHEN. Single nucleotide polymorphisms of myostation gene and its association with growth traits in Haliotis diversicolor supertexta[J].Journal of Tropical Oceanography, 2019, 38(2): 78-85.

Figures/Tables 5

Tab. 1

Fig. 1

Tab. 2

Tab. 3

Fig. 2

References 31

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引物	引物序列(5°—3°)	温度/℃	位置
SNP-1F	GTCAGGTGCGTAGCAAAC	57	173~1003bp
SNP-1R	AGATGATGATGTCGGTTGT	57	173~1003bp
SNP-2F	AGCCTCTTGTTCATTCCTACT	52	1871~2657bp
SNP-2R	ACACCCTTATCTGCTATACCTT	52	1871~2657bp
β-actin-F	TGCCCATCTACGAAGGTTACGCC	60	荧光定量内参(200bp)
β-actin-R	GCTGCTGTGGACATCTCCTGCTC	60	荧光定量内参(200bp)
MSTN-RF	GTGACTCGAATGGACACGGA	60	荧光定量(114bp)
MSTN-RR	GCATCTGACCGCGATACCAT	60	荧光定量(114bp)

位点	等位基因	PIC	N_e	H_e	H_o	HWE p值
5′UTR g252G>A	G(0.6176)/A(0.3824)	0.3608	1.8951	0.4751	0.4353	0.4367
ORF g414A>C g558C>A g909C>T g960A>T	A(0.7882)/C(0.2118) C(0.8000)/A(0.2000) C(0.6905)/T(0.3095) A(0.8780)/T(0.1220)	0.2781 0.2668 0.3360 0.1913	1.5011 1.4706 1.7465 1.2725	0.3358 0.3219 0.4300 0.2155	0.3059 0.2588 0.3810 0.2195	0.4056 0.0673 0.2919 0.8620
3′UTR g2148G>A g2164A>G g2420C>G g2520C>G	G(0.7706)/A(0.2294) A(0.6118)/G(0.3882) C(0.7353)/G(0.2647) C(0.6824)/G(0.3176)	0.2911 0.3621 0.3135 0.3395	1.5469 1.9048 1.6374 1.7652	0.3557 0.4778 0.3916 0.4361	0.3412 0.4471 0.2941 0.3294	0.7074 0.5509 0.0205(p<0.05) 0.2524

位点	基因型	基因型频率	体质量/g	壳长/mm	壳宽/mm
5′UTR
g252G>A	AG(37)	0.41	11.62±4.14	48.76±6.00	30.31±3.64
	AA(13)	0.14	9.42±2.94	45.07±5.25	28.35±2.89
	GG(40)	0.45	11.41±4.47	47.57±7.23	29.42±4.94
ORF
g414A>C	AC(28)	0.31	11.59±4.60	48.35±6.72	30.17±4.00
	AA(56)	0.62	10.79±3.98	47.02±6.63	29.18±4.41
	CC(6)	0.07	13.32±4.14	51.07±5.12	31.40±3.63
g558C>A	AC(23)	0.26	12.49±4.57	49.84±6.34	30.84±3.91
	AA(6)	0.07	11.35±4.48	48.32±5.27	30.15±3.69
	CC(61)	0.68	10.71±3.99	46.83±6.71	29.13±4.38
g909C>T	TC(35)	0.39	11.48±3.34^b	48.57±5.03^ab	30.29±3.03^ab
	CC(43)	0.48	10.26±4.10^b	45.79±6.94^b	28.45±4.69^b
	TT(11)	0.12	14.00±6.00^a	52.05±7.65^a	31.97±4.79^a
g960A>T	TA(17)	0.20	11.47±3.35	48.96±5.25	30.76±2.96
	AA(68)	0.79	11.19±4.46	47.38±7.01	29.54±4.22
	TT(1)	0.01	15.68	53.32	31.83
3′UTR
g2148G>A	AG(29)	0.30	11.85±4.34	48.59±6.39	30.33±4.66
	GG(59)	0.61	10.54±3.98	46.63±6.52	29.03±3.95
	AA(8)	0.08	11.27±3.02	48.33±5.12	30.02±2.93
g2164A>G	AG(46)	0.47	11.49±4.44	48.10±7.31	29.89±4.96
	AA(36)	0.37	10.62±3.81	46.49±5.57	28.92±3.28
	GG(16)	0.16	10.92±3.72	47.66±5.52	30.02±3.31
g2420C>G	CG(27)	0.28	11.99±4.40	48.77±6.51	30.48±4.75
	CC(58)	0.60	10.54±4.02	46.61±6.58	29.02±3.99
	GG(11)	0.11	10.99±2.9	47.9±4.69	29.67±2.77
g2520C>G	GC(34)	0.35	11.00±4.78	47.1±8.01	29.15±5.47
	CC(48)	0.50	11.14±3.76	47.64±5.47	29.71±3.28
	GG(14)	0.15	10.51±3.06	47.07±5.15	29.67±2.92

Single nucleotide polymorphisms of myostation gene and its association with growth traits in Haliotis diversicolor supertexta

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