鬼手海葵(Aiptasia pulchella)白化恢复后的基因表达差异

doi:10.11978/2020143

Abstract

Abstract:

Anemone bleaching can be defined generally as a decline in the population of the symbiotic algae, a reduction in the pigment content of the algae, or both. In order to study the molecular mechanism of Aiptasia pulchella during bleaching and recovered phage affected by chronic heat stress, transcriptome analysis by Illumina Hi-seq of bleaching and recovered A. pulchella was carried out on 50109686 and 43163786 clean reads, which were annotated as 24565 and 24157 unigenes, respectively. The results of comparative transcriptome analysis showed that there were 214 differentially expressed genes (DEGs), including 101 that were significantly highly expressed in bleaching A. pulchella and 113 in recovered A. pulchella. These genes were mainly involved in DNA replication, metabolism, ion transport, and collagen. Gene set enrichment analysis (GSEA) of all 28, 050 expressed genes showed that collagen and ion transport were enriched in bleaching A. pulchella, while nucleotide repair was enriched in recovered A. pulchella. We speculate that these biological processes may play an important role in the bleaching and recovering phases of anemone. The main findings obtained in this study preliminarily reveal the molecular mechanism of adaptive regulation of anemone holobiont after symbiotic imbalance, providing a theoretical basis for the adaptive mechanism of anemone and coral symbiosis system in response to environmental changes.

Key words: Aiptasia pulchella, Transcriptome, Bleaching, Recovery after bleaching

CLC Number:

Q953

ZHANG Qian, YAN Hao, WANG Lu. Difference in gene expression of Aiptasia pulchella after albino recovery[J].Journal of Tropical Oceanography, 2021, 40(6): 111-119.

Figures/Tables 7

Fig. 1

Tab. 1

Fig. 2

Tab. 2

Differentially expressed genes associated with DNA replication, metabolism, ion transport, and collagen"

基因功能	基因编号	AP_W	AP_H	padj	基因注释
与DNA复制相关基因	gene25952	1.69	37.61	<0.01	ATP-dependent DNA helicase PIF1
	gene16761	8.50	64.63	<0.01	ATP-dependent DNA helicase PIF1
	gene20928	52.76	172.71	0.04	ATP-dependent DNA helicase PIF1
	gene1065	0.84	85.78	<0.01	Histone H2B type 1
	novel.1702	27.23	152.74	<0.01	DDE superfamily endonuclease
与新陈代谢相关的基因	gene9753	517.51	125.72	<0.01	Group XV phospholipase A2
	gene4747	52.76	760.13	<0.01	Beta-glucuronidase
	gene21985	68.93	9.41	<0.01	Beta-glucuronidase
	gene4924	1469.14	187.99	<0.01	Carbonic anhydrase 2
	gene5393	204.27	748.38	<0.01	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
	gene12810	634.97	1875.04	0.03	Acetyl-CoA carboxylase
	gene22575	32.33	157.44	<0.01	Aldehyde dehydrogenase family 3 member B1
	gene22820	148.95	37.61	0.01	Chitobiase
	gene24483	52.76	180.94	0.03	D-3-phosphoglycerate dehydrogenase
	gene9970	659.66	132.77	<0.01	Endoglucanase E
	gene898	11.90	189.16	<0.01	Lathosterol oxidase
	gene20346	676.68	2193.42	0.01	Phosphatidylserine decarboxylase proenzyme
与离子转运有关的蛋白	gene4543	128.52	566.28	<0.01	Ammonium transporter Rh type B
	gene4000	520.06	170.36	0.03	Voltage-dependent N-type calcium channel subunit alpha-1B
	gene1518	187.25	56.41	0.04	Organic cation transporter protein
	gene10353	28.08	104.57	0.03	Sodium-dependent neutral amino acid transporter B(0)AT3
	gene13262	780.53	2478.91	0.01	Reticulocalbin-3
	gene23383	0.00	259.65	<0.01	Sodium- and chloride-dependent GABA transporter 3
	gene10062	236.62	65.80	0.01	2-oxoglutarate and iron-dependent oxygenase domain-containing protein 2
与胶原蛋白有关的基因	novel.1027	684.34	180.94	<0.01	Collagen triple helix repeat
	gene24960	18.71	0.00	0.01	Tenascin-R
	gene2049	114.05	353.64	0.03	Matrilin-2
	gene19290	394.09	82.25	<0.01	Collagen alpha-1(X) chain
	gene18911	868.20	112.80	<0.01	Collagen alpha-5(Ⅵ) chain
	gene18914	432.39	74.03	<0.01	Collagen alpha-6(Ⅵ) chain
	gene11395	30.63	2.36	0.01	Collagen triple helix repeat-containing protein 1
	gene1876	421.33	91.65	<0.01	Fibrinogen C domain-containing protein 1
	gene20101	490.27	156.27	0.03	Fibulin-1
	gene19080	117.45	28.21	0.01	Gelsolin-like protein 1
	gene8325	62.12	12.94	0.03	Short-chain collagen C4

Tab. 2

Fig. 3

Fig. 4

Fig. 5

References 28

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样品名称	Raw reads	Clean reads	Clean bases	Error rate	Q20/%	Q30/%	GC pct/%	Gene number	Novel
AP_W	51938216	50109686	7.52G	0.03	96.52	90.82	41.42	24565	1886
AP_H	43920530	43163786	6.47G	0.03	97.86	93.97	43.42	24157	1869

Difference in gene expression of Aiptasia pulchella after albino recovery

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