基于RNA-Seq高通量测序技术的法螺差异表达基因分析

doi:10.11978/2022197

Abstract

Abstract:

The giant triton snail (Charonia tritonis), an endangered gastropod species of ecological and economic importance, is widely distributed in coral reef ecosystems of the Indo-West Pacific region and the tropical waters of the South China Sea. Research on molecular mechanisms is limited due to the lack of complete genomic data for this species. In the present work, transcriptome sequencing of foot muscle and mantle in C. tritonis were obtained by Illumina HiSeq sequencing platform, from which 7994 (3196 upregulated and 4798 downregulated) differentially expressed genes (DEGs) containing biomineralization sequences were identified. The top 20 GO terms in the molecular function category were considered to be related to biomineralization. In KEGG classifications, DEGs are primarily enriched in some pathways that may be involved in biomineralization. The results of qPCR showed that three of the eight genes examined are significantly up-regulated in the mantle. Our results improve the understanding of biomineralization in C. tritonis and provide fundamental transcriptome information to study other molecular mechanisms, such as reproduction.

Key words: Charonia tritonis, transcriptome, DEG

LIU Wenguang, ZHANG Gege, CEN Xitong, HE Maoxian. Analysis of differential expressed genes from Charonia tritonis based on transcriptome sequencing[J].Journal of Tropical Oceanography, 2023, 42(4): 146-154.

Figures/Tables 8

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References 29

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样本名称	总数/个	总长度/bp	平均长度/bp	N50长度/bp	N70长度/bp	GC碱基对数量/%
法螺肌肉	87443	50845674	581	799	426	43.26
法螺外套膜	81100	48450379	597	843	442	43.78
All-Unigene	109722	70289861	640	995	485	43.43

	总数	NR	NT	Swissport	KEGG	KOG	InterPro	GO	Intersection	Overall
基因个数	109722	26050	27059	16944	18705	15319	17617	9788	2712	46239
注释比例	100%	23.74%	24.66%	15.44%	17.05%	13.96%	16.06%	8.92%	2.47%	42.14%

Analysis of differential expressed genes from Charonia tritonis based on transcriptome sequencing

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