比较基因组学分析弧菌属群体感应通路的分布与进化

doi:10.11978/2021025

Abstract

Abstract:

Quorum sensing (QS) is a process of bacterial cell-cell communication in which bacteria can monitor their population density by detection of extracellular autoinducer. QS allows the bacteria to switch between two kinds of gene expression modes: The program is suitable for individual development at low cell density, while favoring community at high cell density. Presently, there are mainly seven kinds of QS signaling molecules, including oligopeptides, AHL (Acylated Homoserine Lactones), AI-2 (Autoinducer-2), CAI-1 (Cholera Autoinducer-1), PQS (Pseudomonas Quinolone Signal), AI-3 (Autoinducer-3), and DSF (Diffusible Signal Factor). Among them, oligopeptides mainly exist in gram-positive bacteria, and the others are commonly found in gram-negative bacteria. Species of the Vibrio genus are important pathogens for human and aquaculture. Since traditional antibiotic treatment has a strong selection pressure leading to increasing number of drug-resistant Vibrio and increasing serious problems, QS quenching is believed as one alternative strategy of the most potential to combat bacterial infection. Thus, it is necessary to investigate the distribution of QS pathways among the Vibrio genus. Among the 46 whole genome sequenced Vibrio species, we found that only three pathways exist in Vibrio species, including AHL, AI-2 and CAI-1 pathways. Specifically, five Vibrio species contain the above three pathways, and 30 strains contain both AI-2 and CAI-1 pathways. However, none of the 46 Vibrio species have the PQS, AI-3 and DSF pathways. In addition, the distribution of QS pathways in Vibrio is related to their phylogeny, suggesting that species with the same QS pathway(s) are close relatives, which indicates that the genes of this pathway evolved from their common ancestor. This study provides useful information for QS quenching against Vibrio pathogens by targeting the AI-2 and CAI-1 QS pathways.

Key words: comparative genomics, vibrio genera, quorum sensing, signaling molecules, species evolution, quorum quenching

CLC Number:

P735.51

MAO Yingjin, GAO Beile. Comparative genomic analysis of the distribution and evolution of quorum sensing pathways in the Vibrio genus[J].Journal of Tropical Oceanography, 2022, 41(1): 28-41.

Figures/Tables 8

Fig. 1

Tab. 1

Tab. 2

Strain information"

物种名称	菌株	基因组编号	基因组大小/Mb	编码序列数目/个
Vibrio alfacsensis	CAIM 1831	GCA_003544875.1	4.91	3830
Vibrio alginolyticus	FA2	GCA_011801435.1	5.25	4598
Vibrio anguillarum	VIB12	GCA_002310335.1	4.90	4091
Vibrio antiquarius	EX25	GCA_000024825.1	5.09	4451
Vibrio aphrogenes	CA-1004	GCA_002157735.2	3.38	2881
Vibrio astriarenae	HN897	GCA_010587385.1	4.80	4176
Vibrio breoganii	FF50	GCA_001677275.1	4.49	3978
Vibrio campbellii	BoB-90	GCA_002906455.1	6.17	5407
Vibrio casei	DSM 22364	GCA_002218025.2	4.14	3601
Vibrio chagasii	ECSMB14107	GCA_004022545.1	5.55	4448
物种名称	菌株	基因组编号	基因组大小/Mb	编码序列数目/个
Vibrio cholerae	10432-62	GCA_000969265.1	4.08	3546
Vibrio cidicii	2756-81	GCA_009763805.1	4.75	3492
Vibrio cincinnatiensis	2070-81	GCA_009763705.1	3.81	3284
Vibrio coralliilyticus	RE22	GCA_003391375.1	5.78	5064
Vibrio cyclitrophicus	ECSMB14105	GCA_005144905.1	5.07	4283
Vibrio diabolicus	FA3	GCA_011801455.1	5.11	4501
Vibrio europaeus	NPI-1	GCA_013154935.1	5.45	4782
Vibrio fluvialis	FDAARGOS_104	GCA_001558415.2	4.83	4338
Vibrio furnissii	FDAARGOS_777	GCA_006364355.1	4.99	4466
Vibrio gazogenes	ATCC 43942	GCA_002196515.1	4.79	4070
Vibrio harveyi	ATCC 33843	GCA_000770115.2	5.88	5166
Vibrio hyugaensis	090810a	GCA_002906655.1	5.61	4845
Vibrio jasicida	090810c	GCA_002887615.1	5.99	5188
Vibrio mediterranei	QT6D1	GCA_002214345.1	5.81	5146
Vibrio metoecus	08-2459	GCA_009665275.1	3.99	3518
Vibrio metschnikovii	9502-00	GCA_009763765.1	3.62	3118
Vibrio mimicus	SCCF01	GCA_001767355.1	4.48	3925
Vibrio natriegens	CCUG 16371	GCA_001680045.1	5.16	4451
Vibrio navarrensis	2462-79	GCA_009763725.1	4.78	4093
Vibrio nigripulchritudo	SFn1	GCA_000801275.1	6.32	5545
Vibrio owensii	XSBZ03	GCA_002021755.1	5.89	5112
Vibrio panuliri	JCM 19500	GCA_009938205.1	4.86	4235
Vibrio ponticus	DSM 16217	GCA_009938225.1	4.80	4098
Vibrio parahaemolyticus	VPD14	GCA_004006515.1	5.17	4531
Vibrio qinghaiensis	Q67	GCA_002257545.1	4.02	3373
Vibrio rotiferianus	B64D1	GCA_002214395.1	5.28	4593
Vibrio rumoiensis	FERM P-14531	GCA_002218045.2	4.21	3605
Vibrio scophthalmi	VS-12	GCA_001685465.1	4.93	4206
Vibrio sp	2521-89	GCA_002216685.1	4.12	3539
Vibrio splendidus	BST398	GCA_003345295.1	5.51	4582
Vibrio taketomensis	C4III291	GCA_009938185.1	4.36	3526
Vibrio tapetis	CECT4600	GCA_900233005.1	5.73	4800
Vibrio tasmaniensis	LGP32	GCA_000091465.1	4.97	4169
Vibrio tritonius	JCM 16456	GCA_001547935.1	5.22	4549
Vibrio tubiashii	ATCC 19109	GCA_000772105.1	5.54	4946
Vibrio vulnificus	07-2444	GCA_009764115.1	5.23	4554

Tab. 2

Tab. 3

Fig. 2

Tab. 4

Tab. 5

Tab. 6

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合成酶	信号分子	受体蛋白	模式菌株	参考文献
LuxI	AHL	LuxR	费氏弧菌 Aliivibrio fischeri	Eberhard et al, 1981
LuxS	AI-2	LuxPQ/LsrB/RbsB	霍乱弧菌 Vibrio cholerae	Bassler et al, 1993
CqsA	CAI-1	CqsS	霍乱弧菌 Vibrio cholerae	Miller et al, 2002
PqsA/B/C/D/E/H	PQS	PqsR	铜绿假单胞菌 Pseudomonas aeruginosa	Pesci et al, 1999
LuxS	AI-3	QseBC/QseEF	大肠杆菌 Escherichia coli O157:H7	Sperandio et al, 2003
RpfF	DSF	RpfCG/RpfR	野油菜黄单胞菌 Xanthomonas campestris	Barber et al, 1997

Comparative genomic analysis of the distribution and evolution of quorum sensing pathways in the Vibrio genus

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