线纹海马肠道菌群结构与功能对迟钝爱德华氏菌(Edwardsiella tarda)感染的响应特征研究

doi:10.11978/2021074

Abstract

Abstract:

Bacterial enteritis can cause severe damage to seahorse aquaculture, while little is known about the effects of bacterial pathogen infection on intestinal microbiota of seahorses. In the present study, both bacterial pathogen (Edwardsiella tarda YT1) and seahorse research model of bacterial enteritis previously reported by us were employed to explore the role of bacterial pathogen in intestinal microbiota by high-throughput full-length 16S rRNA gene sequencing. The results showed that E. tarda infection significantly altered the composition and abundance, and significantly decreased diversity of intestinal microbiota of lined seahorses (p<0.05); significantly increased the relative abundance of Proteobacteria (p<0.05), and decreased the abundance of Actinobacteria, Firmicutes, and Bacteroidetes (p<0.05); significantly increased the relative abundance of pathogenic Edwardsiella (p<0.01), while decreasing that of Psychrobacter, Rothia, Macrococcus, and Planococcus (p<0.05) at genera level. It indicates that E. tarda infection can reduce the diversity and relative abundance of intestinal autochthonous microbiota and result in dysbiosis in lined seahorses. Based on the results of bacterial function and correlation, increasing relatively abundance of E. tarda may significantly upregulate its activities of bacterial chemotaxis, flagella assembly, ABC transporter, phosphotransferase system, and lipopolysaccharide biosynthesis pathways (p<0.05), cause decrease of the relative abundance of the core intestinal microbiota of Psychrobacter, Planococcus and Glutamicibacter and suppression of their functional activities of ribosome, RNA degradation, nucleotide excision repair, and fatty acid biosynthesis pathways (p<0.05), induce dysbiosis of intestinal microbiota, and finally result in enteritis. These results may be helpful for further revealing pathogenic mechanism of E. tarda-induced enteritis in seahorses by more detailed metagenomic and metabolomics analysis.

Key words: seahorse, intestinal microbiota, enteritis, dysbiosis, Edwardsiella tarda

CLC Number:

P735.51

ZHANG Lele, ZOU Qiang, TIAN Yanan, LÜ Chunhui, ZHENG Shiyi, JIANG Guangjun, GAO Longkun, HOU Yuping, WANG Kai. Dysbiosis of both structure and function of intestinal microbiota in lined seahorses (Hippocampus erectus) as response to Edwardsiella tarda infection[J].Journal of Tropical Oceanography, 2022, 41(2): 177-188.

Figures/Tables 9

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样本名称	序列数/bp	碱基数/bp	平均长度/bp	最短序列长度/bp	最长序列长度/bp
Con_1	40963	17215829	420.28	211	478
Con_2	89292	37497350	419.94	208	527
Con_3	53207	22541120	423.65	211	524
ET_1	31899	13629173	427.26	211	432
ET_2	48727	20886268	428.64	211	431
ET_3	36722	15739755	428.62	319	430

名称	Con	ET	p值
Shannon指数	2.59±0.65^a	0.89±0.53^b	0.02
Simpson指数	0.19±0.06^b	0.68±0.28^a	0.04
ACE指数	449.82±31.96	362.46±232.13	0.55
Chao指数	439.46±41.06	326.82±245.5	0.47
物种覆盖度	0.99	0.99	0.39

Dysbiosis of both structure and function of intestinal microbiota in lined seahorses (Hippocampus erectus) as response to Edwardsiella tarda infection

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