基于形态与分子数据对湛江4个江蓠物种(红藻门)的分类学研究*

doi:10.11978/2022085

Abstract

Abstract: According to the molecular and morphological analysis, the species of Gracilariaceae was studied using the samples collected from Zhanjiang, Guangdong province, China. Four Gracilariaceae species were identified, namely: Gracilaria tenuistipitata, G.firma, G.edulis and Gracilariopsis bailinae. Their taxonomic characters and phylogenetic relationships were discussed in detail, and combined with data from the literature, it was clarified that G. tenuistipitata, G. tenuistipitata var. liui and G. longirostris are the same species, and G. firma and G.changii are the same species. This study revised the taxonomic information of Gracilariaceae species in the coastal waters of southern China, and also provided a taxonomic basis for the conservation and development of macroalgae resources in Zhanjiang sea area.

Key words: Zhanjiang, Morphology, Gracilaria, Phylogenetic analysis

LI Nenghui, HUANG Qing, LI Hang, ZENG Jun, WU Kefeng, TAN Huaqiang. Taxonomic study of four species of Gracilaria (Gracilariaceae, Rhodophyta) in Zhanjiang based on morphological and molecular data*[J].Journal of Tropical Oceanography, 0, (): 0-.

References

[1] 丁兰平, 黄冰心, 谢艳齐, 2011. 中国大型海藻的研究现状及其存在的问题[J]. 生物多样性, 19(6): 798-804. DING LANPING, HUANG BINGXIN, XIE YANQI, 2011. Advances and problems with the study of marine macroalgae of China seas[J]. Biodiversity Science, 19(6): 798-804 (in Chinese with English abstract).
[2] 丁兰平, 谭华强, 孙国栋, 等, 2014. 粤东地区江蓠属Gracilaria (江蓠科, 红藻门)的形态分类学[J]. 热带海洋学报, 33(4): 33-39. DING LANPING, TAN HUAQIANG, SUN GUODONG, et al., 2014. The morphological taxonomy on genus Gracilaria (Gracilariaceae, Rhodophyta) from the east coast of Guangdong province in China[J]. Journal of Tropical Oceanography, 33(4): 33-39 (in Chinese with English abstract).
[3] 丁兰平, 黄冰心, 王宏伟, 2015. 中国海洋红藻门新分类系统[J]. 广西科学, 22(2): 164-188. DING LANPING, HUANG BINGXIN, WANG HONGWEI, 2015. New classification system of marine brown algae of China[J]. Guangxi Sciences, 22(2): 164-188 (in Chinese with English abstract).
[4] 丁兰平, 杨楠, 刘金梅, 等, 2020. 海洋经济红藻江蓠科的分类研究现状及存在的问题[J]. 应用海洋学学报, 39(1): 144-147. DING LANPING, YANG NAN, LIU JINMEI, et al., 2020. Taxonomic status and problems on marine economic red algal Family Gracilariaceae[J]. Journal of Applied Oceanography, 39(1): 144-147 (in Chinese with English abstract).
[5] 刘思俭, 2001. 我国江蓠的种类和人工栽培[J]. 湛江海洋大学学报, 21(3): 71-79 (in Chinese).
[6] 李来好, 李刘冬, 杨贤庆, 等, 2003. 江蓠方便食品的加工工艺及毒理的研究[J]. 食品与发酵工业, 29(7): 10-13. LI LAIHAO, LI LIUDONG, YANG XIANQING, et al., 2003. Studies on Technology of Conveniency Food from Gracilaria and Its Toxicology[J]. Food and Fermentation Industries, 29(7): 10-13 (in Chinese with English abstract).
[7] 李文红, 胡自民, 覃志彪, 等, 2004. 细基江蓠及其繁枝变种的RAPD和ITS分析[J]. 海洋学报, 26(6):89-95. LI WENHONG, HU ZIMIN, QIN ZHIBIAO, et al., 2004. RAPD detection and ITS sequences analysis of Gracilaria tenuistipitata and G.tenuistipitata var. liui[J]. Acta Oceanologica Sinica, 26(6): 89-95 (in Chinese with English abstract).
[8] 李方洲, 黄亚冬, 郭永军, 等, 2022. 不同饵料对皱纹盘鲍养殖效果的影响[J]. 饲料研究, 45(7): 56-61. LI FANGZHOU, HUANG YADONG, GUO YONGJUN, et al., 2022. Effect of different feed on breeding effect of wrinkled abalone[J]. Feed Research, 45(7): 56-61 (in Chinese with English abstract).
[9] 潘江球, 李思东, 2010. 江蓠的资源开发利用新进展[J]. 热带农业科学, 30(10): 47-50. PAN JIANGQIU, LI SIDONG, 2010. Development and Utilization of Gracilaria Resources[J]. Chinese Journal of Tropical Agriculture, 30(10): 47-50 (in Chinese with English abstract).
[10] 夏邦美, 1999. 中国海藻志第二卷红藻门第五册伊谷藻目杉藻目红皮藻目[M]. 科学出版社 (in Chinese). (in Chinese).
[11] 谢恩义, 申玉春, 叶宁, 等, 2009. 流沙湾的底栖大型海藻调查[J]. 广东海洋大学学报, 29(4): 30-35. XIE EnYI, SHEN YUCHUN, YE NING, et al., 2009. Benthic marine macroalgae survey in Liusha Gulf[J]. Journal of Guangdong Ocean University, 29(4): 30-35 (in Chinese with English abstract).
[12] 杨宇峰, 费修绠, 2003. 大型海藻对富营养化海水养殖区生物修复的研究与展望[J]. 青岛海洋大学学报, 33(1): 53-57. YANG YUFENG, FEI XIUGENG, 2003. Prospects for bioremediation of cultivation of large-sized seaweed in eutrophic mariculture areas[J]. Journal of Ocean University of Qingdao, 33(1): 53-57 (in Chinese with English abstract).
[13] 张峻甫, 夏邦美, 1962. 中国江蓠属植物地理学的初步研究[J]. 海洋与湖沼, 4(3-4): 189-198. ZHANG JUNFU, XIA BANGMEI, 1962. A preliminary phytogeographical studies on Chinese species of Gracilaria[J]. Oceanologia et Limnologia Sinica, 4(3-4): 189-198 (in Chinese with English abstract).
[14] 张峻甫, 夏邦美, 1963. 江蓠科的一个新属—多穴藻属[J]. 海洋科学集刊, 3: 119-126 (in Chinese).
[15] 张峻甫, 夏邦美, 1976. 中国江蓠属海藻的分类研究[J]. 海洋科学集刊, 11: 91-163 (in Chinese).
[16] 张才学, 周伟男, 孙省利, 等, 2020. 硇洲岛大型海藻群落的季节演替[J]. 热带海洋学报, 39(1): 74-84. ZHANG CAIXUE, ZHOU WEINAN, SUN XINGLI, et al., 2020. Seasonal succession of macroalgae community in Naozhou Island[J]. Journal of Tropical Oceanography, 39(1): 74-84 (in Chinese with English abstract).
[17] BHUSHAN S, VEERAGURUNATHAN V, BHAGIYA B K, et al., 2023. Biology, farming and applications of economically important red seaweed Gracilaria edulis (S. G. Gmelin) P. C. Silva: A concise review[J]. Journal of Applied Phycology, 35(3): 983-996.
[18] DAWSON E Y, 1949. Studies of Northeast Pacific Gracilariaceae[J]. Occasional Papers of the Allan Hancock Foundation, 7: 1-105.
[19] FRESHWATER D W, RUENESS J, 1994. Phylogenetic relationships of some European Gelidium (Gelidiales, Rhodophyta) species, based on rbcL nucleotide sequence analysis[J]. Phycologia, 33(3): 187-194.
[20] GMELIN S G, 1768. Historia fucorum[M]. Ex typographia Academiae scientiarum, p. 1-253.
[21] GURGEL C F D, LIAO L M, FREDERICQ S, et al., 2003. Systematics of Gracilariopsis (Gracilariales, Rhodophyta) based on rbcL sequence analyses and morphological evidence[J]. Journal of Phycology, 39(1): 154-171.
[22] GURGEL C F D, FREDERICQ S, 2004. Systematics of the Gracilariaceae (Gracilariales, Rhodophyta): a critical assessment based on rbcL sequence analysis[J]. Journal of Phycology, 40: 138-159.
[23] GANESAN M, SAHU N, ESWARAN K, 2011. Raft culture of Gracilaria edulis in open sea along the south-eastern coast of India[J]. Aquaculture, 321(1): 145-151.
[24] GURGEL C F D, NORRIS J N, SCHMIDT W E, et al., 2018. Systematics of the Gracilariales (Rhodophyta) including new subfamilies, tribes, subgenera, and two new genera, Agarophyton gen. nov. and Crassa gen. nov[J]. Phytotaxa, 374(1): 1-23.
[25] GURGEL C F D, SOARES L P, NORRIS J N, et al., 2020. Molecular systematics of Crassiphycus and Hydropuntia (Gracilariales, Rhodophyta) with the description of poorly known taxa in the Western Atlantic Ocean[J]. European Journal of Phycology, 56(2): 216-229.
[26] HALL T A, 1999. BioEdit: A user-friendly biological sequencealignment editor and analysis program for Windows95/98/NT[J]. Nucleic Acids Symposium Series, 41(41): 95-98.
[27] LEWMANOMONT K, 1994. The species of Gracilaria from Thailand[C] // ABBOTT I A. In: Taxonomy of economic seaweeds with reference to some Pacific species. California Sea Grant College, 4: 135-148.
[28] LE N H, NGUYEN H D, 2006. Contribution to the study of Gracilaria and relative genera (Gracilariales, Rhodophyta) from Vietnam[J]. Coast Mar Sci, 30(1): 214-221.
[29] LYRA G, NUNES J, PESTANA E, et al., 2021. Diversity of Gracilariaceae (rhodophyta) in brazil: integrating morphological and molecular data[J]. Phytotaxa, 496(1): 1-53.
[30] MONTAGNE J F C, 1842. Algae[C] // DE LA SAGRA R, ed. Histoire physique, politique et naturelle de l’Ile de Cuba. Botanique-plantes cellulaires, 1-104.
[31] MCDEVIT D C, SAUNDERS G W, 2009. On the utility of DNA barcoding for species differentiation among brown macroalgae (Phaeophyceae) including a novel extraction protocol[J]. Phycological Research, 57(2): 131-141.
[32] NÄGELI C, 1847. Die neuern Algensysteme und Versuch zur Begründung eines eigenen Systems der Algen und Florideen[M]. Friedrich Schulthess.
[33] NGUYEN H D, 1992. Vietnamese species of Gracilaria and Gracilariopsis[C] // ABBOTT I A. Taxonomy of economic seaweeds with reference to some Pacific and Caribbean species. California: California Sea Grant College Program, 3: 207-210.
[34] NG P K, LIN S M, LIM P E, et al., 2017. Genetic and morphological analyses of Gracilaria firma and G. changii (Gracilariaceae, Rhodophyta), the commercially important agarophytes in western Pacific[J]. PLoS One, 12(7): e0182176.
[35] POSADA D, BUCKLEY T R, 2004. Model selection and model averaging in phylogenetics: Advantages of akaike information criterion and Bayesian approaches over likelihood ratio tests[J]. Systematic Biology, 53(5): 793-808.
[36] RONQUIST F, HUELSENBECK J P, 2003. MrBayes3: Bayesian phylogenetic inference under mixed models[J]. Bioinformatics, 19(12): 1572-1574.
[37] SILVA P C, 1952. A review of nomenclatural conservation in the algae from the point of view of the type method[J]. University of California Publications in Botany, 25: 241-323.
[38] SAUNDERS G W, 2008. A DNA barcode examination of the red algal family Dumontiaceae in Canadian waters reveals substantial cryptic species diversity. 1. The foliose Dilsea-Neodilsea complex and Weeksia[J]. Botany, 86(7): 773-789.
[39] TERADA R, KAWAGUCHI S, MASUDA M, et al., 2000. Taxonomic notes on marine algae from Malaysia Ⅲ. Seven species of Rhodophyceae[J]. Bot Mar, 43: 347-357.
[40] TAMURA K, NEI M, KUMAR S, 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method[J]. Proceedings of the National Academy of Sciences, 101(30): 11030-11035.
[41] TAMURA K, STECHER G, KUMAR S, 2021. MEGA11: Molecular Evolutionary Genetics Analysis version 11[J]. Molecular biology and evolution, 38(7): 3022-3027.
[42] WHEELER Q D, 2005. Losing the plot: DNA “barcodes” and taxonomy[J]. Cladistics, 21(4): 405-407.
[43] WANG X L, GUOA M L, YANA S S, et al., 2023. Diversity of Gracilariaceae (Rhodophyta) in China: An integrative morphological and molecular assessment including a description of Gracilaria tsengii sp. Nov[J]. Algal Research, 71: 103074.
[44] XIA B M, ABBOTT I A, 1987. New species of Polycavernosa Chang & Xia (Gracilariaceae, Rhodophyta) from the western Pacific[J]. Phycologia, 26: 405-418.
[45] YAMAMOTO H, 1978. Systematical and anatomical study of the genus Gracilaria in Japan[J]. Mem Fac Fish Hokkaido Univ, 25: 97-152.
[46] YANG M Y, DONG J D, KIM M S, 2012. Taxonomic notes on five species of Gracilariaceae from Hainan, China[J]. Algae, 27(3): 175-187.
[47] ZHANG J F, XIA B M, 1988. On two new Gracilaria (Gigartinales, Rhodophyta) from South China[C] // ABBOTT I A. Taxonomy of economic seaweeds with reference to some Pacific and Caribbean species. California: California Sea Grant College Program, 2: 132-134.
[48] ZHANG J F, XIA B M, 1991. A new specific name in the genus Gracilaria[J]. Chinese Journal of Oceanology and Limnology, 9(3): 290.
[49] ZHANG J F, XIA B M, WANG Y C, et al., 1995. Additions to the species of Gracilaria of China[C] // ABBOTT I A. Taxonomy of economic seaweeds with reference to some Pacific and Caribbean species. California: California Sea Grant College Program, 5: 197-202.

Taxonomic study of four species of Gracilaria (Gracilariaceae, Rhodophyta) in Zhanjiang based on morphological and molecular data*

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	LI Nenghui, LYU Zhengwen, CAI Hongyan, LI Hang, ZENG Jun, HE Haiping, ZHU Yuzhen, WU Kefeng, ZHAO Wei, TAN Huaqiang, LUO Hui. Morphological and molecular identification of five Gracilaria species (Gracilariaceae, Rhodophyta) from the Zhanjiang coastal area [J]. Journal of Tropical Oceanography, 2025, 44(6): 74-90.
[2]	GUO Youyou, XIE Enyi, XIN Rong, LIN Kun, LIAO Jiawei, CHEN Chunli, WANG Huihui, YANG Wencheng, CUI Jianjun, MO Feng, WEN Qijiao. Algae-fish interactions and wastewater quality control in recirculating aquaculture systems [J]. Journal of Tropical Oceanography, 2025, 44(2): 147-156.
[3]	ZHOU Zhiqian, LIAN Xiping, TAN Yehui. Taxonomic research of the genus Hansarsia from the South China Sea^* [J]. Journal of Tropical Oceanography, 2025, 44(2): 100-114.
[4]	HAO Yanxia, ZHAO Yuxuan, DENG Liqiu, ZHANG Ruixue, WANG Shuhong. Observation on the asexual reproduction of Phyllorhiza sp. [J]. Journal of Tropical Oceanography, 2025, 44(1): 35-43.
[5]	ZHOU Zhigang, YUE Wen, LI Huiquan, LIN Yangyang. Influence of tree species and intertidal elevations on the carbon storage of the Gaoqiao mangrove area in Zhanjiang, Guangdong Province [J]. Journal of Tropical Oceanography, 2024, 43(2): 108-120.
[6]	LI Nenghui, HUANG Qing, LI Hang, ZENG Jun, WU Kefeng, TAN Huaqiang. Taxonomic study of four species of Gracilaria (Gracilariaceae, Rhodophyta) in Zhanjiang based on morphological and molecular data [J]. Journal of Tropical Oceanography, 2024, 43(2): 34-47.
[7]	HAN Tong, LI Jingjing, LIU Zhengyi, LIU Kai, Zhang Jinhao, QIN Song, ZHONG Zhihai. The seed morphology and internal characteristics of seagrass, surfgrass Phyllospadix iwatensis [J]. Journal of Tropical Oceanography, 2022, 41(6): 105-113.
[8]	LIU Wei, GUO Haipeng, DONG Pengsheng, YAN Mengchen, ZHANG Demin. Draft genome sequence and comparative genome analysis of Alliroseovarius sp. Z3 [J]. Journal of Tropical Oceanography, 2022, 41(1): 52-61.
[9]	LIU Jinmei, HUANG Bingxin, DING Lanping, WANG Xuecong, YAN Jing, YAN Panzhu, ZHANG Yao. Morphological and phylogenetic studies of Yonagunia formosana in southeastern Hainan province, China [J]. Journal of Tropical Oceanography, 2021, 40(6): 76-82.
[10]	SHI Yonghao, JIA Liangwen, ZHANG Heng, LIN Yitong. Environmental capacity calculation and sewage treatment in Inner Zhanjiang Bay [J]. Journal of Tropical Oceanography, 2021, 40(4): 134-142.
[11]	LONG Chao, LUO Zhaohe, WEI Zhangliang, YANG Fangfang, LI Ru, LONG Lijuan. Morphology and phylogeny of zooxanthellae Effrenium voratum from Luhuitou reef in Sanya, Hainan province [J]. Journal of Tropical Oceanography, 2021, 40(4): 35-43.
[12]	NIU Biaobiao, ZHAI Mengyi, LI Yang. Morphological and Phylogenetic Studies on Chaetoceros seychellarus [J]. Journal of Tropical Oceanography, 2021, 40(4): 44-49.
[13]	LIU Jinmei, JIANG Jingjing, MA Xin, HUANG Bingxin, YANG Nan, LIU Meiyuan, DING Lanping. Morphological taxonomy of genus Hypnea (Rhodophyta, Gigartinales) from eastern Guangdong, China [J]. Journal of Tropical Oceanography, 2021, 40(1): 99-110.
[14]	Lifen HUANG, Qun LI, Songhui LÜ, Liang ZHANG, Xuedong XIE. Morphology, phylogeny and toxicity of Coolia tropicalis (Dinophyceae) from the Xisha Islands, China [J]. Journal of Tropical Oceanography, 2020, 39(3): 86-97.
[15]	Lichun JIANG, Qun LI, Songhui LÜ. Morphology and Phylogenetics study on the species of Amphidinium (Gymnodinials, Dinophyceae) from Weizhou Island, Guangxi [J]. Journal of Tropical Oceanography, 2020, 39(3): 106-115.