南沙群岛珊瑚礁区黑斑鹦嘴鱼(Scarus globiceps)食性分析

doi:10.11978/2023102

Abstract

Abstract:

The parrotfish (Scaridae) plays an important role in maintaining the stability and balance of coral reef ecosystems, participating in many key ecological processes. Due to limitations in previous research methods, there is insufficient understanding of the food sources and ecological functional value of parrotfish, leading to controversies regarding their functional role. In this study, the typical areas of coral distribution, the Dongmen Reef and the Nanxun Reef in the Nansha Islands, were selected as the study sites, and the algal diversity consumed by the dominant species of parrotfish, Scarus globiceps, in this region was comprehensively analyzed. High-throughput sequencing analysis of the DNA in the intestinal contents of S. globiceps was conducted using both 18S rDNA and 16S rDNA. The sequencing results of 18S rDNA revealed that the intestinal contents of S. globiceps consisted mainly of several types of eukaryotic algae, including Dinoflagellata, Rhodophyta, Chlorophyta, and Ochrophyta, with a total of 77 operational taxonomic units (OTUs). Dinoflagellata had relatively high sequence abundance and diversity, accounting for 51.42% of the total eukaryotic algae sequences, with one species from the family Suessiaceae exceeding 20% in both the Dongmen Reef and Nanxun Reef samples. The sequencing of 16S rDNA identified sequences of prokaryotic algae (cyanobacteria) in the intestinal contents, with a total of 21 OTUs, and the relative sequence abundance of the order Nostocales was the highest, reaching 39.33%. This study indicates that although S. globiceps consumes a certain amount of macroalgae during feeding, microalgae (dinoflagellates and cyanobacteria) still dominate, suggesting the need to reconsider the important contribution of microalgae to the diet of parrotfish and their impact on the structure and function of coral reef ecosystems.

Key words: parrotfish, dietary analysis, high-throughput sequencing, 18S rDNA, 16S rDNA, dinoflagellata, cyanobacteria

LIN Xianzhi, ZHOU Yanyan, LIN Haoye, HU Simin, HUANG Hui, ZHANG Li, LIU Sheng. Diet analysis of the parrotfish (Scarus globiceps) in coral reefs of the Nansha Islands[J].Journal of Tropical Oceanography, 2024, 43(3): 100-108.

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编号	站点	取样数	体重/g	体长/cm
Scagl. D	东门礁	8	60.33±17.04	11.91±1.15
Scagl. N	南薰礁	8	55.03±26.33	11.61±2.11

Diet analysis of the parrotfish (Scarus globiceps) in coral reefs of the Nansha Islands

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