Journal of Tropical Oceanography ›› 2010, Vol. 29 ›› Issue (4): 76-81.doi: 10.11978/j.issn.1009-5470.2010.04.076cstr: 32234.14.j.issn.1009-5470.2010.04.076

• Marine biology • Previous Articles     Next Articles

Breeding and miniaturizing screening of rotifer Brachionus plicatilis by applying UV radiation and mutagenic agents

GUO Feng1,2, LIU Xue-dong1, CAI Ze-ping1, LIN Yue-guang1, ZHANG Jun-bin1   

  1. 1. Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, CAS, Guangzhou 510301, China; 2.Graduate University of CAS, Beijing 100039, China
  • Received:2008-04-02 Revised:2008-05-28 Online:2010-07-31 Published:2010-07-29
  • Contact: 蔡泽平。 E-mail:czeping@sohu.com
  • About author:郭峰(1981—), 男, 内蒙古包头市人, 硕士研究生, 主要从事海洋生物技术研究。Email: fengge11998811@126.com
  • Supported by:

    广东省中国科学院战略合作项目(2009B091300156); 广东省科技计划项目(2006B20201054); 广东省海洋渔业科技专项(A2009004-004)

Abstract:

Ultraviolet (UV) radiation and mutagenic agents, including colchicine and ethyl methane sulfonate (EMS), are applied in the mutation breeding and miniaturizing screening of Brachionus plicatilis. B. plicatilis shows a decrease in incubation rate, oocyte production rate and individual sizes with the rising of UV radiation and the densities of mutagenic agents. When the UV radiation reaches 4.8´104-6.0´104μW·s·cm-2, there is a distinct and rapid decline of the incubation rate. When the UV radiation reaches1.2´105-1.5´105μW·s·cm-2, both rates start to decline more slowly. The average body length of rotifers in the group treated with UV is 200nm, which is 50nm shorter than the control group. Length in rotifer long axis, short axis and oocyte axis decrease by 20%, 14% and 7.7% in the group treated with 20mg·L-1 colchicine and by and 30%, 19% and 12%, with 10% (volume percentage concentrations) EMS, respectively. The best radiation dose and the density of the mutagenic agents are radiant intensity 400μW·cm-2, radiant time 2 minutes, and final radiation dose 5.0´104μW·s·cm-2. The optimal densities of the mutagenic agents are colchicines of 2mg·L-1, EMS of 0.6% (volume percentage concentrations), and time of steeping over 12 hours.

Key words: Brachionus plicatilis, ultraviolet radiation, colchicines, ethyl methane sulfonate, miniaturizing screening