Journal of Tropical Oceanography ›› 2010, Vol. 29 ›› Issue (5): 168-173.doi: 10.11978/j.issn.1009-5470.2010.05.168cstr: 32234.14.j.issn.1009-5470.2010.05.168

• Marine biology • Previous Articles     Next Articles

Ultra-structures of peripheral blood cells of Chetonia mydas

LI Chang-ling1, CAO Fu-jun1, HUANG Xiang-hu1, LIU Chu-wu1, WU Jian-jun1, LIU Shao-jun2   

  1. 1. Fisheries Collge, Duangdong Ocean University, Zhanjiang, 524025, China; 2. Collge of Life Sciences, Hunan Normal University, Changsha, 410081, China
  • Received:2008-10-06 Revised:2009-10-29 Online:2010-10-30 Published:2011-10-10
  • Contact: 刘楚吾。 E-mail:liucw@gdou.edu.cn
  • About author:李长玲, (1964—), 女, 河南省武陟县人, 教授, 主要从事水生生物学研究。E-mail: ybcl@tom.com
  • Supported by:

    国家科技支撑计划资助项目(2007BAD29B03)

Abstract:

The peripheral blood cells of Chetonia mydas Linnaeus were observed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). TEM results showed that the erythrocyte had oval nuclear and no organoids with high dense electrons. Lymphocyte was characterized by the heterochromatin congregating in the middle of the nuclear and contained only a few granules and vacuoles in the cytoplasm. Monocyte was characterized by a horseshoe-shaped nuclear with abundant heterochromatin, and its cytoplasm with many vacuoles near the outside part of cytoplasma had small quantities of little azurophilic granule. Basophil characterized by a “V” shape, and sublobe nuclear had a kind of round and dense specific granule scattered in the cytoplasm. Eosinophil by an irregular oval nuclear with abundant heterochromatin had specific and azurophilic granules. Neutrophil was characterized by two types of granules. SEM results displayed that erythrocyte was ob-long with smooth surface. Lymphocyte was characterized by many stubbed and dense microvillus. Monocyte had fold surface, more obvious than that of B- Lymphocyte. Macrophage with an irregular shape often holding other cells by the pseudopod was larger than other cells. Thrombocyte, irregular formed, had acerose process and usually congregated together. Neutrophil was characterized by rough surface and irregular drape. Leucocytes had easy desquamatory drapes.

Key words: Chetonia myda, blood cell, transmission electron microscopy, scanning electron microscopy