斜带石斑鱼烂尾病病原菌的分离与鉴定

doi:10.11978/j.issn.1009-5470.2010.06.118

Journal of Tropical Oceanography ›› 2010, Vol. 29 ›› Issue (6): 118-124.doi: 10.11978/j.issn.1009-5470.2010.06.118cstr: 32234.14.j.issn.1009-5470.2010.06.118

• Marine biology • Previous Articles Next Articles

Isolation and identification of bacteria pathogens from Epinephelus coioides with tail-rotted disease

MEI Bing, ZHOU Yong-can, XU Xian-dong, WANG Shi-feng, XIE Zhen-yu

College of Marine Science, Hainan University, Haikou 570228, China

Received:2009-02-10 Revised:2009-11-15 Online:2010-12-15 Published:2010-12-15
Contact: 谢珍玉。 E-mail:xiezyscuta@163.com
About author:梅冰(1982—), 男, 湖北省武汉市人, 硕士研究生, 研究方向为水生生物病害及其控制。E-mail: meibing11meibing@163.com
Supported by:
农业科技成果转化项目(2009GB2E200302); 国家自然科学基金项目(30660144、30760190、31060360); 海南省自然科学基金
项目(80617); 海南省教育厅高等学校科研项目(Hj200729)

Abstract

Abstract:

A very serious tail-rotted disease broken out in the cage-cultured Epinephelus coioides in Sanya city and Lingshui County, Hainan province, China in May 2008. Three strains of HS08001, HS08002, and XC08001 were isolated from the dis-eased grouper and were shown to be the pathogens by artificial infection. All the three isolates were identified as Vibrio har-veyi by biochemical tests and 16S rDNA sequence analysis. The LD50 value of strain HS08001 for E. coioides was 1.05×104 CFU/g body weight. Sensitivity test to 25 chemotherapeutic agents indicated that the pathogen was only highly sensitive to gentamicin, chloramphenicol, and rifampicin.

Key words: Epinephelus coioides, Vibrio harveyi, tail-rotted disease, 16s rDNA

MEI Bing,ZHOU Yong-can,XU Xian-dong,WANG Shi-feng,XIE Zhen-yu. Isolation and identification of bacteria pathogens from Epinephelus coioides with tail-rotted disease[J].Journal of Tropical Oceanography, 2010, 29(6): 118-124.

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Isolation and identification of bacteria pathogens from Epinephelus coioides with tail-rotted disease

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