线纹海马(Hippocampus erectus)性腺特异性结构与生殖细胞发育特征研究 *

doi:10.11978/2020014

Abstract

Abstract:

Seahorses (Genus Hippocampus) have not only peculiar reproductive strategy of male pregnancy but also unique ovarian and testis structures. Developmental characteristics of the ovary and testis as well as the germ cells of the lined seahorse (Hippocampus erectus) were clarified in the present study. Based on the histological structure and morphological features, we described six stages of gonadal development of lined seahorse. Seahorses have an asynchronous ovarian developmental characteristic. The stageⅠovary contained only oogonia and early meiotic oocytes (d<20 μm). In the stage Ⅱ ovary, phase Ⅱ oocytes (d=20-140 μm) began to appear. In the stage Ⅲ ovary, the number and volume of the phase Ⅲ oocytes (d=140-260 μm) were increased remarkably. The vitellogenic oocytes (d=260-1100 μm), in which transitional yolk sphere was accumulated, could be observed in the stage Ⅳ ovary. Phase Ⅴ maturational oocytes (d=1100-2000 μm), which were characterized by highly compact vitelline envelope and fluid yolk mass, were observed in the stage Ⅴ ovary. The ovary shortly after ovulation with decrement of the number of oocytes was stage Ⅵ ovary. The testis of the lined seahorse is of the unrestricted lobular type. Stage Ⅰ testis mainly contained spermatogonia with large nuclear and one or more nucleolus. In the stage Ⅱ testis, the spermatocyst began to appear, inside of which were the primary spermatocytes with smaller nuclear. The secondary spermatocytes were first present in the stage Ⅲ testis. In the stage Ⅳ testis, the volume of testis was increased significantly and the matured spermatids appeared. Large scale of mature spermatozoa was restricted into the testicular lumen and ready to be released in the stage Ⅴ testis. The stage Ⅵ testis was vascularized with few spermatozoa or spermatocytes in the lumen. Further histochemical analysis showed that the amino acid and fat were positive in the cytoplasm and yolk mass of oocytes, and accumulated with the oocytes development. Generally, our results provide basic evidence for further understanding of male pregnancy strategies of seahorses.

Key words: Hippocampus erectus, gonad development, histological structure, ovary, testis

CLC Number:

Q953

CHEN Lingzhen, QIN Geng, WANG Xin, LIU Yali, XIAO Wanghong, LIN Qiang. Distinct structures of gonads and germ cell development of lined seahorse, Hippocampus erectus[J].Journal of Tropical Oceanography, 2020, 39(6): 93-102.

Figures/Tables 4

Tab. 1

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生长时期/d	体重/g	体长/cm	卵巢	精巢
14	0.03±0.01	2.20±0.13	性腺不可见	性腺不可见
21	0.04±0.01	2.59±0.10	肉眼可见	肉眼不可见
30	0.15±0.02	3.37±0.74	肉眼可见	肉眼可见
40	0.33±0.14	4.52±0.70	性腺细丝状, 透明, 组织切片可辨	性腺细丝状, 透明, 组织切片可辨
55	0.41±0.07	5.11±0.41	肉粉色, 性腺增粗, 细丝状, 肉眼可辨	透明, 性腺增长, 细丝状, 肉眼可辨
70	0.55±0.08	5.55±0.20	肉粉色至浅黄色, 性腺带状, 较精巢略短	透明, 性腺增长, 带状
90	2.14±0.73	8.36±0.93	淡橘色, 性腺增粗, 圆柱状, 较短, 含少量卵黄颗粒	乳白色, 性腺增粗增长, 管状, 结缔组织增多
120	4.42±0.80	9.75±1.93	橘色, 性腺增大, 蛹状, 卵黄颗粒增多, 但不能分离	乳白色, 性腺进一步增长、增粗, 管状, 结缔组织增厚
150	5.08±1.74	10.01±0.77	深橘色, 性腺体积进一步增大, 蛹状, 卵黄颗粒增多, 饱满	乳白色, 性腺依然在增粗、增长, 长管状, 结缔组织增厚, 肉眼可见血管
180	9.67±1.28	13.58±0.65	橘红色, 性腺体积进一步增大, 蛹状, 卵粒饱满, 卵子可从卵巢中挤出	乳白色, 性腺圆管状, 较粗, 精液可从精巢中挤出, 肉眼可见血管
250	10.30±0.19	13.80±0.22	深橘红色, 性腺体积变小, 松弛, 含皱缩空泡, 含卵黄颗粒, 但不能分离	乳白色, 性腺圆管状, 较粗, 肉眼可见血管

Distinct structures of gonads and germ cell development of lined seahorse, Hippocampus erectus

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