Fig. 1

Schematic diagram of chemotaxis system The letters represent different chemotaxis proteins; FliM is flagellum motor protein; Pi stands for phosphate group"

Tab. 1

Strain information"

Tab. 2

Plasmid information"

Tab. 3

Campylobacter jejuni 81-176 related genetic information"

基因	基因编号	蛋白结构域示意图
cheV	CJJ81176_0311
cheW	CJJ81176_0309
tlp1	CJJ81176_1498
tlp2	CJJ81176_0180
tlp3#	CJJ81176_1548
tlp3#	CJJ81176_1549
tlp4	CJJ81176_0289
tlp5*	CJJ81176_0271-4	Pseudogene
tlp6	CJJ81176_0473
tlp7	CJJ81176_0975
tlp8	CJJ81176_1128
tlp9	CJJ81176_1205
tlp10	CJJ81176_0046
aer1	CJJ81176_1204
aer2	CJJ81176_1206

Tab.4

Primer sequence"

Fig. 2

Using bacterial two-hybrid system to study the interaction between CheV and chemotactic receptors in Campylobacter jejuni 81-176 (a) Schematic diagram of bacterial two-hybrid experiment; (b) Schematic diagram of gene cloning. The left panel shows that for transmembrane Tlps, only MA domains and sequences after the MA domain were cloned into vectors. The right panel shows the full-length sequence of the cytolasmic chemotactic receptor was selected for cloning; (c) Interaction of CheV with Tlps by bacterial two-hybrid experiments; (d) Interaction of CheW with Tlps by bacterial two-hybrid experiments. The shade of color in Figure C and D represents the strength of protein interaction. BTH101 is a strain for bacterial double hybrid system"

Tab. 5

Analysis and statistics of F-Class and CheV in deep sea Epsilon-proteobacteria"

Fig. 3

Pairwise Distances matrix to calculate the evolutionary distance for CheV homologs between deep-sea Epsilon-proteobacteria and Campylobacter jejuni Blue indicates the evolutionary distance between deep-sea Epsilon- proteobacteria. Orange indicates the evolutionary distance between deep-sea Epsilon-proteobacteria and C. jejuni 81-176. Dot shows the distance between F3-CheV in different Epsilon-proteobacteria, and triangle shows the distance between F8-CheV in sulfurimonas autotrophica and F3-CheV in other Epsilon-proteobacteria"

Fig. 4

Sequence alignment of CZB domain and CZB-like domain Blue font indicates deep-sea strain, Red letter indicates conserved amino acids of CZB domain"

Tab. 6

Analysis and statistics of CZB domain in deep sea Epsilon-proteobacteria"

Fig. 5

Study on Zn-binding capacity and potential function of CZB-like domain (a) Zn concentration measure by ICP-mass; (b) Using the average Zn concentration and average protein concentration, the binding ratio of protein and Zn is calculated; (c and d) To investigate whether the CZB-like domain of Tlp9 can affect the interaction with CheV or CheW by bacterial two-hybrid experiments. The shade of color in Figure C and D represents the strength of protein interaction"