一株群体感应抑制活性海洋放线菌的筛选与鉴定 *

doi:10.11978/2020011

Abstract

Abstract:

Quorum Sensing Inhibitors (QSIs) are promising alternatives to antibiotics, which can reduce the infectivity and virulence of pathogenic bacteria by interruption of quorum sensing (QS) pathways. The strategy aimed at disarming rather than at eradicating bacterial pathogens, and imposed no strong selective pressure on bacteria that can lead to the development of resistance mechanisms. Marine microorganism is one of the most potential sources of novel QSIs. In the present study, actinomycetes strains, isolated from the deep-sea sediment samples obtained from the South China Sea and Indian Ocean, were tested by Chromobacterium violaceum 026 (CV026) model, for screening for QS inhibitory strains. Strain SCSIO 53717 showed inhibitory activity against quorum sensing of CV026. The extract of SCSIO 53717 significantly reduced the production of CV026 violacein, and the extract of the strain showed no inhibitory effects on the growth of CV026 strain within the concentration range from 0 to 1000 mg·mL^-1. This strain was assigned to the genus Kocuria based on morphological characteristics and 16S rRNA gene sequence analysis. To the best of our knowledge, this is the first time that Kocuria is reported to be QS inhibitory active. This result indicates that strain SCSIO 53717 is worthy further studying.

Key words: quorum sensing, marine actinomycete, quorum sensing inhibitory active, Chromobacterium violaceum 026, 16S rRNA, genus Kocuria

CLC Number:

P735.51

LI Yanqun, CHEN Rouwen, LIN Zonghao, TIAN Xinpeng, YIN Hao. Screening and identification of a quorum sensing inhibitory actinomycetes derived from marine sediments[J].Journal of Tropical Oceanography, 2021, 40(1): 75-81.

Figures/Tables 6

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References 22

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Screening and identification of a quorum sensing inhibitory actinomycetes derived from marine sediments

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