一株红树林根际固氮菌的分离、鉴定以及固氮活性测定

doi:10.11978/j.issn.1009-5470.2010.05.149

Journal of Tropical Oceanography ›› 2010, Vol. 29 ›› Issue (5): 149-153.doi: 10.11978/j.issn.1009-5470.2010.05.149cstr: 32234.14.j.issn.1009-5470.2010.05.149

• Marine biology • Previous Articles Next Articles

Isolation and characterization of a N2-Fixing Bacterium from the mangrove rhizosphere and study on its nitrogen-fixing ability

LING Juan1, 2, 3,4, DONG Jun-de1,3, ZHANG Yan-ying 1,3, YANG Zhi-hao1, WANG You-shao2

1. Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; 2. Key Laboratory of Tropical Marine Environmental Dynamics, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; 3. National Experiment Station of Tropical Marine Biology, Sanya 572000, China; 4. Graduate school of Chinese academy of science, Beijing 100049, China

Received:2008-12-05 Revised:2009-02-06 Online:2010-10-30 Published:2011-10-10
Contact: 董俊德 E-mail:dongjunde@vip.163.com.
About author:凌娟(1985—), 女, 山东省临沂市人, 博士研究生, 主要从事海洋生态、微生物培养及分子生物学研究。E-mail: jingling0224@ 163.com
Supported by:
中国科学院知识创新项目(KSCX2?SW?132); 国家自然科学基金(40776069、40676091); 国家生态系统研究网络三亚站
数据库与信息系统, 中科院生态系统研究网络台站研究基金; 海洋公益性行业科研专项经费项目(200705026); 广东省科
技计划项目(2004B60302004, NO.2005B60301032); 重大基础研究前期研究专项(2005CCA04800); 广东省海洋药物重
点实验室开放课题; 中国科学院南海海洋研究所LMM-LAMB-LMB联合开放课题。

Abstract

Abstract:

A bacterial strain H9 capable of nitrogen fixation was isolated from the mangrove rhizosphere using N-free medium. It was identified as Bacillus pumilus by type description, physiological and biochemical test, G+C Mole fraction (%), 16S rRNA sequence analysis and the nif H sequence analysis. It was a gram-positive, straight-rodded bacterium, which forms white colony with diameter of 3-4 mm on the solid agar medium. Compared with the standard strain of the species B. pumilus, there were high similarity in the carbon source utility, hydrolysis and the optimal growth temperature and salinity. The G+C content of the strain DNA was 44.6%. A phylogenetic tree was constructed by comparing validly published 16S rRNA sequences of the related strains in the Genebank, using the neighbor-joining method. It showed that the strain H9 and the strain B. pumilus B402 have the highest similarity, which is 99.6%. The measurement of nitrogen-fixing ability by acetylene reduction was 156.32 nmol C2H4•H?1•mL?1, indicating its high nitrogen-fixing ability.

Key words: mangrove, nitrogen-fixing bacterium, 16S rRNA, nif H

LING Juan,DONG Jun-de,ZHANG Yan-ying ,YANG Zhi-hao,WANG You-. Isolation and characterization of a N2-Fixing Bacterium from the mangrove rhizosphere and study on its nitrogen-fixing ability[J].Journal of Tropical Oceanography, 2010, 29(5): 149-153.

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Isolation and characterization of a N2-Fixing Bacterium from the mangrove rhizosphere and study on its nitrogen-fixing ability

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