马氏珠母贝异速生长个体对免疫刺激的差异响应

doi:10.11978/2021095

Abstract

Abstract:

In this study, we investigated the immune performance of allometric individuals of the pearl oyster, Pinctada fucata martensii, compared the expression of immune-related genes (CuZn-SOD1, CuZn-SDO2, LAMP1, JRL, and Rab7) and changes in the activities of three immune enzymes (SOD, AKP and CAT) in large and small individuals after immune stimulation, and analyzed the differential response of allometric individuals to immune stimulation. The results showed that the immune-related genes and immune enzymes all responded to the immune stimuli. The relative expression levels of LAMP1, CuZn-SOD1 and CuZn-SOD2 genes and the changes of CAT and AKP activities were higher in small individuals than those in large individuals, while the relative expression levels of JRL and Rab7 genes and the changes of SOD activity were lower than those in large individuals. The basal expression of the JRL gene was much higher in the small individuals than that in the large ones, which may result in a smaller variation. These results indicated that the small individuals were more responsive to external stresses and had stronger immune response ability.

Key words: Pinctada fucata martensii, allometric individuals, immune stimulation, gene expression, enzyme activity

CLC Number:

P735.54

XU Hanzhi, ZHANG Hua, XIONG Panpan, HE Maoxian. Differential responses of allometric individuals to immune stimuli in Pinctada fucata martensii[J].Journal of Tropical Oceanography, 2022, 41(5): 180-188.

Figures/Tables 3

References 27

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引物名称	引物序列
CuZn-SOD1-F	ACAAATAATTCGGCATCCTA
CuZn-SOD1-R	TAGAGCCTGGTTTGGTGTAT
CuZn-SOD2-F	ATAGGTTCAAACGGAGGAGG
CuZn-SOD2-R	TGGTCGCAAATGGTTGTATT
JRL-F	ATAGGTTCAAACGGAGGAGG
JRL-R	TGGTCGCAAATGGTTGTATT
LAMP-1F	TGCCTGTCACAATAATAACC
LAMP1-R	AGACTCAAAGTAAGACCACCT
Rab7-F	GAGTTTAGGCGTGGCTTTCT
Rab7-R	ATCCCTTGGACTTGCTTGTAT
LAMP2-F	ACGAAAAAAAGGTTTGAGAGACG
LAMP2-R	AGATACTTGATGGCCTGTTG
18S-F	CGTTTCAACAAGACGCCAGTAG
18S-R	ACGAAAAAAAGGTTTGAGAGACG

Differential responses of allometric individuals to immune stimuli in Pinctada fucata martensii

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