热带海洋学报 ›› 2024, Vol. 43 ›› Issue (6): 190-195.doi: 10.11978/2024003CSTR: 32234.14.2024003

• 海洋生物学 • 上一篇    下一篇

印度洋深海来源真菌Penicillium sp. SCSIO 06871次级代谢产物研究

庞小艳1,2(), 李晓琳1,2, 王俊锋1,2, 田新朋1,2, 徐石海3, 刘永宏1,2()   

  1. 1.中国科学院南海海洋研究所, 中国科学院热带海洋生物资源与生态重点实验室, 广东 广州 510301
    2.中国科学院大学, 北京 100049
    3.暨南大学化学与材料学院, 广东 广州 510632
  • 收稿日期:2024-01-04 修回日期:2024-02-22 出版日期:2024-11-10 发布日期:2024-12-05
  • 通讯作者: 庞小艳, 刘永宏
  • 作者简介:

    庞小艳(1990—), 女, 河北省邢台市人, 助理研究员, 博士, 从事海洋天然药物化学研究。email:

  • 基金资助:
    广州市科技计划项目(202201010678); 海南省科技计划三亚崖州湾科技城联合项目(2021JJLH0097); 国家自然科学基金项目(42006084)

Study on the secondary metabolites from Penicillium sp. SCSIO 06871 of the deep-sea Indian Ocean

PANG Xiaoyan1,2(), LI Xiaolin1,2, WANG Junfeng1,2, TIAN Xinpeng1,2, XU Shihai3, LIU Yonghong1,2()   

  1. 1. Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
    2. University of Chinese Academy of Sciences, Beijing 100049, China
    3. College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, China
  • Received:2024-01-04 Revised:2024-02-22 Online:2024-11-10 Published:2024-12-05
  • Contact: PANG Xiaoyan, LIU Yonghong
  • Supported by:
    Guangzhou Science and Technology Project(202201010678); Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City(2021JJLH0097); National Natural Science Foundation of China(42006084)

摘要:

本文对印度洋深海来源真菌Penicillium sp. SCSIO 06871的次级代谢产物进行了研究。采用硅胶、Sephadex LH-20凝胶、和反相ODS (octadecylsilyl)柱色谱、及半制备高效液相色谱法(high performance liquid chromatography, HPLC)分离等手段对该菌株的大米固体培养基发酵产物进行分离纯化, 结合核磁共振、质谱、旋光等谱学方法对分离得到的化合物进行结构鉴定, 共鉴定7个聚酮类化合物(17)和1个吲哚生物碱类化合物(8), 结构分别为: sydowinin B(1)、mrlactone1(2)、AGI-B4(3)、methyl cordyol C(4)、diorcinol(5)、11-dehydrosydonic acid(6)、(+)-sydonic acid(7)和indole-3-acetate(8)。抑菌活性实验显示, 化合物4, 58对多株致病细菌均显示出不同程度的抑制活性。同时α-葡萄糖苷酶抑制活性实验显示, 化合物58均有较强的α-葡萄糖苷酶抑制活性。

关键词: 深海沉积物来源真菌, Penicilliumsp., 次级代谢产物, 抗菌, α-葡萄糖苷酶抑制活性

Abstract:

The rice fermentation products of the fungus Penicillium sp. SCSIO 06871 from the deep-sea Indian Ocean were studied in this paper. The extract was isolated and purified by silica, Sephadex LH-20, and reversed phase octadecylsilyl (ODS) column chromatography, as well as semipreparative high performance liquid chromatography (HPLC). Eight known compounds, including sydowinin B (1), mrlactone1 (2), AGI-B4 (3), methyl cordyol C (4), diorcinol (5), 11-dehydrosydonic acid (6), (+)-sydonic acid (7), and methyl indole-3-acetate (8) were obtained and identified by analyzing their nuclear magnetic resonance (NMR) spectra, mass spectrum (MS) spectra and specific rotation values. Compounds 4, 5 and 8 showed different degree of inhibitory activities against multiple pathogenic bacteria. Also, compounds 5-8 all showed inhibitory activities against α-glycosidase.

Key words: deep-sea-derived fungus, Penicillium sp., secondary metabolite, antibacterial, inhibitory activity against α-glycosidase

中图分类号: 

  • Q936