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热带海洋学报

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北部湾软珊瑚共附生真菌Scopulariopsis sp. SCSIO 41229次级代谢产物研究

何燕春1, 2, 徐淑娜1, 2, 刘永宏1, 杨斌1   

  1. 1. 中国科学院南海海洋研究所热带海洋生物资源与生态重点实验室, 广东 广州 510301;

    2. 中国科学院大学, 北京 100049

  • 收稿日期:2024-09-29 修回日期:2024-12-02 接受日期:2024-12-12
  • 通讯作者: 杨斌
  • 基金资助:

    国家自然科学基金项目(42276128)

Study on the secondary metabolites of the soft coral-derived fungus Scopulariopsis sp. SCSIO 41229 from the Beibu Gulf

HE Yanchun1, 2, XU Shuna1, 2, LIU Yonghong1, YANG Bin1   

  1. 1. Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;

    2. University of Chinese Academy of Sciences, Beijng 100049, China

  • Received:2024-09-29 Revised:2024-12-02 Accepted:2024-12-12
  • Contact: Yang, Bin
  • Supported by:

    National Natural Science Foundation of China (42276128)

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摘要： 本文旨在研究北部湾软珊瑚共附生真菌Scopulariopsis sp. SCSIO 41229的次级代谢产物。采用正相硅胶柱层析、反相硅胶柱层析、薄层色谱、高效液相色谱等多种方法对该菌株的发酵产物进行分离纯化， 通过核磁共振、质谱等波谱学方法并结合理化性质， 文献数据比对对其进行结构鉴定。从中分离得到9个化合物， 分别为neocyclocitrinols A–D (1~4)、penicillenol A2 (5)、 环(D-苯丙-L-异亮)二肽 (6)、 cyclo-(L-tryptophyl-L-phenylalanyl) (7)、 2-(hept-5-enyl)-3-methyl-4-oxo-6,7,8,8a-tetrahydro-4H-pyrrolo [2,1-b]-1,3-oxazine (8)和 (8E,10E)-7-oxo-8,10-heptadecadienoic acid (9)。生物活性测试显示， 化合物2和4 具有弱抗氧化活性， 化合物1、 4和8 具有弱乙酰胆碱酯酶抑制活性。化合物1~9均为首次从Scopulariopsis sp.真菌中分离得到。

关键词: 珊瑚共附生真菌, 次级代谢产物, Scopulariopsis sp.

Abstract: In this study, we aim to investigate the secondary metabolites from the coral-derived fungus Scopulariopsis sp. SCSIO 41229, which were collected from the Beibu Gulf. The secondary metabolites were isolated by silica gel column chromatography, reverse phase silica gel column chromatography, thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC). The chemical structures of these compounds were identified by nuclear magnetic resonance (NMR), mass spectrometry (MS) and literature analysis. Nine known compounds were isolated and elucidated as neocyclocitrinols A–D (1~4), penicillenol A2 (5), cyclo-(D-Phe-L-Ile) (6), cyclo-(L-tryptophyl-L-phenylalanyl) (7), 2-(hept-5-enyl)-3-methyl-4-oxo-6,7,8,8a-tetrahydro-4H-pyrrolo[2,1-b]-1,3-oxazine (8), (8E,10E)-7-oxo-8,10-heptadecadienoic acid (9). Bioassays indicated that compounds 2, 4 had weak antioxidant activity and compounds 1, 4, 8 showed weak inhibitory activity against acetylcholin esterase. Compounds 1~9 were obtained from the fungus Scopulariopsis sp. for the first time.

Key words: coral-derived fungus, secondary metabolites, Scopulariopsis sp.