丛生盔形珊瑚光合生理及共生真菌群落分析

doi:10.11978/2021064

热带海洋学报 ›› 2022, Vol. 41 ›› Issue (2): 132-141.doi: 10.11978/2021064CSTR: 32234.14.2021064

丛生盔形珊瑚光合生理及共生真菌群落分析

朱文涛^1,³(), 夏景全^2,³, 刘相波^2,³, 尹洪洋^2,³, 朱铭^2,³, 任瑜潇^2,³, 谢敏睿², 黄建中^1,³, 李秀保^2,³()

1. 海南大学生态与环境学院, 海南海口570228
2. 海南大学海洋学院, 海南海口570228
3. 海南大学南海海洋资源利用国家重点实验室, 海南海口 570228

收稿日期:2021-05-19 修回日期:2021-07-16 出版日期:2022-03-10 发布日期:2021-07-26
通讯作者: 李秀保
作者简介:朱文涛(1995—), 男, 安徽省安庆市人, 博士研究生。email: 2274430217@qq.com
基金资助:
国家自然科学基金(42076108);国家自然科学基金(42161144006);海南省重点研发计划(ZDYF2020177)

Analysis of photosynthetic physiology and symbiotic fungi community in Galaxea fascicularis

ZHU Wentao^1,³(), XIA Jingquan^2,³, LIU Xiangbo^2,³, YIN Hongyang^2,³, ZHU Ming^2,³, REN Yuxiao^2,³, XIE Minrui², HUANG Jianzhong^1,³, LI Xiubao^2,³()

1. College of Ecology and Environment, Hainan University, Haikou 570228, China
2. College of Marine Science, Hainan University, Haikou 570228, China
3. State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China

Received:2021-05-19 Revised:2021-07-16 Online:2022-03-10 Published:2021-07-26
Contact: LI Xiubao
Supported by:
National Natural Science Foundation of China(42076108);National Natural Science Foundation of China(42161144006);Hainan Provincial Key Research and Development Program(ZDYF2020177);Foundation of Hainan University(KYQD(ZR)1805)

摘要/Abstract

摘要：

文章以丛生盔形珊瑚Galaxea fascicularis为研究目标, 对其在健康和白化两种状态下的虫黄藻密度、光合生理指标和共生真菌群落进行分析比较。结果发现白化状态下的虫黄藻密度和最大光化学量子产量(F_v/F_m)比健康状态下显著降低了75%和16%。健康和白化状态下珊瑚的共附生真菌均以子囊菌门Ascomycota、担子菌门Basidiomycota、壶菌门Chytridiomycota、毛霉门Mucoromycota为主, 但健康状态下共生真菌的Shannon多样性指数稍低于白化状态下的。健康珊瑚的虫黄藻密度受到热应激威胁而显著降低, 但仍然在合适的共生体密度范围之内。尽管白化珊瑚共生虫黄藻密度降低, 但其光合电子传递系统并没有完全遭到破坏。白化珊瑚真菌的多样性指数、真菌病原体类群及致病功能未显著增加, 显示热胁迫下珊瑚共生真菌群落的稳定性可能在热应激反应中发挥着重要作用。

关键词: 丛生盔形珊瑚, 白化, 虫黄藻密度, 共生真菌, 群落结构

Abstract:

Differences in zooxanthellae density, photosynthetic physiological indicators, and symbiotic fungal communities between healthy and bleaching states of Galaxea fascicularis were investigated in this study. The results showed that the zooxanthellae density and maximum photochemical quantum yield (F_v/F_m) of Galaxea fascicularis in the bleaching state were significantly lower by 75% and 16%, respectively, than those in the healthy corals. The composition of endolithic fungi community was mainly Ascomycota, Basidiomycota, Chytridiomycota, and Mucoromycota. The Shannon diversity index of endolithic fungi in healthy corals was slightly lower than that in bleaching corals, and the endolithic fungi compositions of these two coral groups had some similarity. Zooxanthellae content of healthy corals was significantly reduced due to the threat of heat stress, but it was still within the recommended appropriate symbiont density range. Despite the significant lost of density of symbiotic zooxanthellae from bleached corals, their Photosystem Ⅱ complexes were not completely destroyed. The results showed that there was no significant increase in diversity index, fungal pathogen group and predictive function in bleaching corals. The stability of endolithic fungi community may play an important role in the heat stress response. In the future, we will consider combining traditional isolation and culture techniques, high-throughput sequencing, metagenomics, and other methods to comprehensively study the diversity and ecological functions of coral endolithic fungi.

Key words: Galaxea fascicularis, bleaching, zooxanthellae density, endolithic fungi, community structure

中图分类号:

P732.42

朱文涛, 夏景全, 刘相波, 尹洪洋, 朱铭, 任瑜潇, 谢敏睿, 黄建中, 李秀保. 丛生盔形珊瑚光合生理及共生真菌群落分析[J]. 热带海洋学报, 2022, 41(2): 132-141.

ZHU Wentao, XIA Jingquan, LIU Xiangbo, YIN Hongyang, ZHU Ming, REN Yuxiao, XIE Minrui, HUANG Jianzhong, LI Xiubao. Analysis of photosynthetic physiology and symbiotic fungi community in Galaxea fascicularis[J]. Journal of Tropical Oceanography, 2022, 41(2): 132-141.

图/表 6

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	美夏海域	黄龙海域	排浦海域
纬度/N	20°00′06″	19°55′30″	19°38′30″
经度/E	109°39′05″	109°31′25″	109°07′39″
温度/℃	32.53±0.93^a	31.68±1.08 ^a	31.41±0.16 ^a
pH	8.07±0.04 ^a	8.07±0.05 ^a	8.10±0.02 ^a
盐度/‰	32.02±0.36 ^a	31.77±0.18 ^b	32.30±0.13^a
硅酸盐/(mg?L^-1)	0.56±0.03 ^a	0.57±0.05 ^a	0.43±0.06 ^b
溶解态无机氮/(mg?L^-1)	0.27±0.04 ^a	0.32±0.05 ^a	0.13±0.04 ^b
磷酸盐/(mg?L^-1)	0.03±0.00 ^a	0.02±0.01 ^a	0.01±0.00 ^b

丛生盔形珊瑚光合生理及共生真菌群落分析

Analysis of photosynthetic physiology and symbiotic fungi community in Galaxea fascicularis

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