刺巨藤壶精子的发生及其超微结构

doi:10.11978/2019082

Abstract

Abstract:

The ultrastructural characteristics of spermatogenesis and spermatozoon of the barnacle Megabalanus volcano were observed by scanning and transmission electron microscopy. The results showed that its spermatogenesis included five developmental stages, i.e., spermatogonium, primary spermatocyte, secondary spermatocyte, spermatid, and mature spermatozoon. During spermatogenesis, a circular or oval nucleus extended along with an axial filament and bent in cytoplasm under the action of microtubules. The nucleus further elongated with longitudinal extension of a spermatid, and finally became filiform. Massive chromatin of nucleus underwent disintegration, fine granules, coarse granules, and eventually condensed into a high electron density homogenizer. The vesicles secreted by the Golgi complex coalesced to different sizes of precursors of accessory droplet, and finally fused into an accessory droplet of high electron density. Many small mitochondria fused to a slender one that was eventually positioned behind the nucleus. The spermatozoon (about 58.39 μm in length), which was divided into acrosome, neck, middle piece, and tail, was long filiform with an accessory droplet. The conical acrosome was located at the spermatozoon anterior, followed by a basal body. The neck was composed of a "9+2" type axial filament, and the filiform nucleus was parallel to the axial filament. The length of acrosome and neck was about 6.65 μm. The middle piece was about 42.95 μm in length. The spindle-shaped accessory droplet (about 9.33 μm long and 2.13 μm wide) was adjacent to the nucleus and axial filament, followed by a mass of glycogenosomes and a slender mitochondrion. The tail (about 8.79 μm in length) only had a typical "9+2" axial filament.

Key words: Megabalanus volcano, spermatogenesis, spermatozoon, ultrastructure, accessory droplet

Huan ZHOU, Gang LIN, Xiaozhen RAO. Ultrastructure of spermatogenesis and spermatozoon of Megabalanus volcano[J].Journal of Tropical Oceanography, 2020, 39(3): 98-105.

Figures/Tables 4

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Tab. 1

References 28

1	陈奇成, 蒋霞敏, 韩庆喜 , 等, 2019. 虎斑乌贼精子发生及精子超微结构[J]. 宁波大学学报(理工版), 32(2):1-8.
	CHEN QICHENG, JIANG XIAMIN, HAN QINGXI , et al, 2019. Spermatogenesis and sperm ultrastructure of Sepia pharaonis[J]. Journal of Ningbo University (Natural Science and Engineering Edition), 32(2):1-8 (in Chinese with English abstract).
2	丁汉波, 仝允栩, 黄浙 , 1987. 发育生物学[M]. 北京: 科学出版社: 55-60.
	DING HANBO, TONG YUNXU, HUANG ZHE , 1987. Developmental biology[M]. Beijing: Science Press: 55-60(in Chinese).
3	堵南山, 赖伟, 陈立侨 , 等, 1999. 蟹类精子超微结构的比较研究[J]. 动物学研究, 20(5):372-378.
	DU NANSHAN, LAI WEI, CHEN LIQIAO , et al, 1999. Studies on the comparative ultrastructure of crab spermatozoa (Crustacea, Decapoda, Reptantia, Brachyura)[J]. Zoological Research, 20(5):372-378 (in Chinese with English abstract).
4	葛少钦, 康现江, 郭明申 , 等, 2008. 中国明对虾精子形成过程中顶体的发生[J]. 台湾海峡, 27(1):51-57.
	GE SHAOQIN, KANG XIANJIANG, GUO MINGSHEN , et al, 2008. Development of acrosome during spermiogenesis of Fenneropenaeus chinensis (Osbeck)[J]. Journal of Oceanography in Taiwan Strait, 27(1):51-57 (in Chinese with English abstract).
5	管卫兵, 宣富君, 戴小杰 , 等, 2009. 东海三疣梭子蟹精子发生及精荚形成[J]. 中国水产科学, 16(2):173-182.
	GUAN WEIBING, XUAN FUJUN, DAI XIAOJIE , 2009. Spermatogenesis and spermatophore formation of swimming crab (Portunus trituberculatus Miers, 1876) in East China Sea[J]. Journal of Fishery Sciences of China, 16(2):173-182 (in Chinese with English abstract).
6	韩帅帅, 曹文浩, 严涛 , 等, 2018. 雷州半岛东部近海浮标大型固着生物污损特点[J]. 海洋与湖沼, 49(4):851-857.
	HAN SHUAISHUAI, CAO WENHAO, YAN TAO , et al, 2018. Biofouling by sessile macro-organisms on offshore buoys east of the Leizhou Peninsula, the Northern South China Sea[J]. Oceanologia Et Limnologia Sinica, 49(4):851-857 (in Chinese with English abstract).
7	林岗, 饶小珍, 陈文列等, 2003. 龟足精子发生和成熟精子的超微结构[J]. 应用与环境生物学报, 9(4):417-421.
	LIN GANG, RAO XIAOZHEN, CHEN WENLIE , et al, 2003. Ultrastructure of spermatogenesis and spermatozoom in Pollicipes mitella (Cirripedia)[J]. Chinese Journal of Applied and Environmental Biology, 9(4):417-421 (in Chinese with English abstract).
8	刘瑞玉, 任先秋 , 2007. 中国动物志: 第四十二卷-无脊椎动物 (甲壳动物亚门, 蔓足下纲, 围胸总目)[M]. 北京: 科学出版社: 513-515.
	LIU RUIYU, REN XIANQIU , 2007. Fauna sinica: Volume 42; Invertebrata (Crustacea, Cirripedia, Thoracica)[M]. Beijing: Science Press: 513-515(in Chinese).
9	王兰, 堵南山, 赖伟 , 2000. 长江华溪蟹精子的超微结构[J]. 动物学报, 46(2):227-229.
	WANG LAN, DU NANSHAN, LAI WEI , 2000. Ultrastructure of sperm in freshwater crab (Sinopotamon yangtsekiense)[J]. Acta Zoologica Sinica, 46(2):227-229 (in Chinese with English abstract).
10	王玉凤, 杨晓菁, 朱朝兵 , 等, 2003. 罗氏沼虾精子发生的超微结构[J]. 华中师范大学学报(自然科学版), 37(4):531-536, 539.
	WANG YUFENG, YANG XIAOJING, ZHU CHAOBING , et al, 2003. Ultrastructure of spermatogenesis in freshwater prawn, Macrobrachium rosenbergii (Crustacea: Decapoda)[J]. Journal of Central China Normal University (Natural Sciences), 37(4):531-536, 539 (in Chinese with English abstract).
11	王艺磊, 张子平, 李少菁 , 1998. 甲壳动物精子学研究概况Ⅱ. 精子发生与精子的生化组成[J]. 动物学杂志, 33(4):52-57.
	WANG YILEI, ZHANG ZIPING, LI SHAOJING , 1998. A survey of spermology in crustaceans Ⅱ. Spermatogenesis and sperm biochemical composition[J]. Chinese Journal of Zoology, 33(4):52-57 (in Chinese).
12	王艺磊, 张子平, 李少菁 , 1999. 纹藤壶精子发生和成熟精子的超微结构[J]. 动物学报, 45(3):355-358.
	WANG YILEI, ZHANG ZIPING, LI SHAOJING , 1999. Ultrastructure of spermatogenesis and spermatozoon in Balanus amphitrite amphitrite[J]. Acta Zoologica Sinica, 45(3):355-358 (in Chinese with English abstract).
13	许星鸿, 阎斌伦, 郑家声 , 等, 2012. 甲壳动物精子生物学的研究进展[J]. 海洋湖沼通报, ( 1):6-14.
	XU XINGHONG, YAN BINLUN, ZHENG JIASHENG , et al, 2012. A review on biological characteristic of crustacean sperm[J]. Transactions of Oceanology and Limnology, ( 1):6-14 (in Chinese with English abstract).
14	严涛, 黎祖福, 胡煜峰 , 等, 2012. 中国沿海无柄蔓足类研究进展[J]. 生态学报, 32(16): 5230-5241 doi: 10.5846/stxb201202130185
	YAN TAO, LI ZUFU, HU YUFENG, et al, 2012. A review on the balanomorph barnacles in the coastal waters of China[J]. Acta Ecologica Sinica, 32(16):5230-5241 (in Chinese with English abstract). doi: 10.5846/stxb201202130185
15	郑学斌, 张清科, 乐韵 , 等, 2018. 香鱼(Plecoglossus altivelis)精子的超微结构及其与鲤形目及鲑形目其他鱼类精子结构的比较研究[J]. 海洋与湖沼, 49(4):866-872.
	ZHENG XUEBIN, ZHANG QINGKE, YUE YUN , et al, 2018. Scanning and transmission electron microscopic observation of spermatozoon in Plecoglossus altivelis[J]. Oceanologia Et Limnologia Sinica, 49(4):866-872 (in Chinese with English abstract).
16	ADIYODI K G, ADIYODI R G , 1983. Reproductive biology of invertebrates: Volume II; Spermatogenesis and sperm function[M]. New York: John Wiley and Sons: 407-449.
17	AZEVEDO C, CORRAL L , 1982. Ultrastructural study of spermatozoon and spermiogenesis of Pollicipes cornucopia (Crustacea; Cirripedia), with special reference to nucleus maturation[J]. Journal of Submicroscopic Cytology, 14(4):641-654.
18	BARNES H, KLEPAL W, MUNN E A , 1971. Observations on the form and changes in the accessory droplet and motility of the spermatozoa of some cirripedes[J]. Journal of Experimental Marine Biology and Ecology, 7(2):173-196. doi: 10.1016/0022-0981(71)90030-X
19	BENTO M A G, LÓPEZ GRECO L S, ZARA F J , 2019. Seminal fluid production and sperm packaging in dromiid crabs (Brachyura, Podotremata)[J]. Zoology, 132:17-30. doi: 10.1016/j.zool.2018.09.001 pmid: 30736926
20	BROWN G G , 1970. Some comparative aspects of selected crustacean spermatozoa and crustacean phylogeny[M] // BACCETTI B. Comparative spermatology. New York: Academic Press: 183-203.
21	DIONISIO M, RODRIGUES A, COSTA A , 2007. Reproductive biology of Megabalanus azoricus (Pilsbry), the Azorean Barnacle[J]. Invertebrate Reproduction and Development, 50(3):155-162. doi: 10.1080/07924259.2007.9652240
22	HEALY J M, ANDERSON D T , 1990. Sperm ultrastructure in the Cirripedia and its phylogenetic significance[J]. Records of the Australian Museum, 42(1):1-26. doi: 10.3853/j.0067-1975.42.1990.104
23	KUBO M, NAKASHIMA S, TSUKAHARA J , et al, 1979. Spermiogenesis in barnacles with special reference to organization of the accessory body[J]. Development, Growth and Differentiation, 21(5):445-456. doi: 10.1111/dgd.1979.21.issue-5
24	LÓPEZ D A, LÓPEZ B A, PHAM C K , et al, 2010. Barnacle culture: background, potential and challenges[J]. Aquaculture Research, 41(10):e367-e375. doi: 10.1016/j.jhsa.2016.07.100 pmid: 27569783
25	MARTÍNEZ-SOLER F, KURTZ K, CHIVA M , 2007. Sperm nucleomorphogenesis in the cephalopod Sepia officinalis[J]. Tissue and Cell, 39(2):99-108. doi: 10.1016/j.tice.2007.01.005 pmid: 17379266
26	MUNN E A, BARNES H , 1970. The fine structure of the spermatozoa of some cirripedes[J]. Journal of Experimental Marine Biology and Ecology, 4(3):261-286. doi: 10.1016/0022-0981(70)90039-0
27	NUNES C, RODEIA J, PAULINO B , et al, 2017. Larval rearing of the giant Azorean barnacle, Megabalanus azoricus (Pilsbry, 1916): feeding trials, larval development and settlement on artificial substrata[J]. Aquaculture Research, 48(6):2812-2826. doi: 10.1111/are.2017.48.issue-6
28	POLJAROEN J, VANICHVIRIYAKIT R, TINIKUL Y , et al, 2010. Spermatogenesis and distinctive mature sperm in the giant freshwater prawn, Macrobrachium rosenbergii (De Man, 1879)[J]. Zoologischer Anzeiger, 249(2):81-94. doi: 10.1016/j.jcz.2010.03.002

种类	长度/μm	宽度/μm	形状	内部结构	研究出处
刺巨藤壶( Megabalanus volcano)	9.33	2.13	纺锤形	致密均匀	本文
红巨藤壶(M. rose)	6.5	2.5	柠檬形	致密均匀	Kubo et al, 1979
巨藤壶(M. ajax)	6.5~7.5	2.26~2.3	—	致密均匀	Healy et al, 1990
白脊管藤壶(Fistulobalanus albicostatus)	5.0	0.8	香肠形	中央高密度、四周低密度	Kubo et al, 1979
三角藤壶(Balanus trigonus)	5.5	0.7	纺锤形	中央高密度、四周低密度	Kubo et al, 1979
纹藤壶(Amphibalanus Amphitrite)	17~20	0.7	—	多个囊状致密斑	Healy et al, 1990 王艺磊等, 1999

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Ultrastructure of spermatogenesis and spermatozoon of Megabalanus volcano

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