广西北部湾海域织锦巴非蛤精巢发育、精子发生及超微结构研究

doi:10.11978/2023090

Abstract

Abstract:

In this study, anatomical observation, spermary tissue section, scanning and transmission electron microscopy techniques were used to study the spermary development, spermatogenesis and ultrastructure of mature sperm of Paphia textile in Beibu Gulf of Guangxi. The results showed that no hermaphrodites were found in the collected samples, and all the observed individuals were dioecious. The spermary development of P. textile was divided into five main stages: proliferating stage, growing stage, maturing stage, spawning stage, and resting stage. The maturing stage and spawning stage of P. textile was from September to January of the following year. Sperm development is divided into five stages: spermatogonia stage, primary spermatocyte stage, secondary spermatocyte stage, spermatid stage and mature sperm stage. The mature sperm of P. textile consisting of the head, middle and tail, overall length approximately 46.04 μm. The sperm head is divided into the acrosome and the nucleus. The acrosome is conical in shape and the nucleus is long and cylindrical. The anterior end was 0.71 μm in diameter and the posterior end was about 1.34 μm in diameter. The middle part consisted of four mitochondria encircled in a circular shape and two mutually perpendicular proximal and distal centrioles. The tail flagellum was about 38.8 μm long and 0.21 μm in diameter, and the external plasma membrane was covered with a typical “9 + 2” duplex microtubule structure. The results of this study provide basic research information for the reproductive biology, artificial seed breeding and germplasm resource conservation of P. textile.

Key words: Paphia textile, spermary development, spermatogenesis, ultrastructural, histological section

XU Bingjie, LIU Yiming, XING Qinggan, LIAN Changpeng, WU Tao, PAN Ying. Spermary development, spermatogenesis and sperm ultrastructure of Paphia textile in the Beibu Gulf, Guangxi[J].Journal of Tropical Oceanography, 2024, 43(2): 59-68.

Figures/Tables 8

Tab.1

Fig. 1

Tab. 2

Fig. 2

Tab. 3

Development characteristics of the spermatid of P. textile at various stages"

发育分期	出现时期及位置	细胞特点
精原细胞	精巢发育各个阶段均可观察到精原细胞, 增殖期较多。由滤泡壁上的上皮细胞增殖而来, 分布在滤泡基膜和生精小管附近。可分为 A型和B型精原细胞。A型细胞一般贴附在滤泡壁或者基膜上, B型分布在滤泡各个部位或紧贴A型精原细胞。	精原细胞形状为圆形或椭圆形, 体积在精子发育过程中最大。A型精原细胞胞内物质少、核仁显现, 但不位于细胞中央, 直径在5.04~6.43μm。B型胞内物质趋于紧密、核仁不明显, 直径在4.89~6.98μm。此期精原细胞染色较浅。
生长初级精母细胞	在精巢发育的生长期较多, 成熟期也存在。生长初级精母细胞由精原细胞A型分化而来, 主要分布在生精小管附近, 靠近滤泡壁。	形状为椭圆形或者圆形, 直径在3.56~4.78μm, 比精原细胞小, 细胞核不位于细胞的中央。
生长次级精母细胞	生长期和成熟期数量最多, 分布不规律, 在生精小管附近。	形状为椭圆形或者圆形, 细胞直径比初级精母细胞小, 直径在2.12~3.87μm, 此期细胞的核膜、核仁不清晰, 细胞核染色质开始浓缩。
精细胞	成熟期、排放期可观察到较多的精细胞, 分布在滤泡中生精小管周围。	精细胞分为前期、中期、后期3个时期。前期主要为椭圆形或者圆形, 至中期大部分转变为椭圆形, 后期为成熟精子。此期染色质高度浓缩, 染色程度高。
成熟精子	在成熟期和排放期成熟精子数量最多, 分布在生精小管上, 以头部朝向滤泡壁、尾部聚集成束朝向滤泡腔中心。	精细胞经过复杂变化形成具有特殊结构的精子。成熟精子呈子弹头形状, 头部粗、尾部细。染色质高度浓缩, 染色程度深。在滤泡中呈菊花状散布, 聚集成束。

Tab. 3

Fig. 3

Fig. 4

Tab. 4

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参数	壳长/mm	壳宽 /mm	壳高/mm	体质量/g	软体质量/g
平均值±标准差	62.73±5.39	23.45±2.62	36.90±3.41	40.05±11.90	8.59±2.99
最大值	73.97	27.03	42.05	67.89	11.14
最小值	52.10	16.60	29.32	20.45	4.16

取样日期	肥满度	精巢发育分期					总数/个	盐度/‰	水温/℃
取样日期	肥满度	Ⅰ	Ⅱ	Ⅲ	Ⅳ	Ⅴ	总数/个	盐度/‰	水温/℃
2020-11-29	25.20			8	5	1	14	27.4	22.0
2020-12-21	21.09			7	2		9	27.5	17.2
2021-01-08	18.33			1	5	2	8	29.4	15.6
2021-02-24	16.64				2	8	10	30.9	17.3
2021-03-18	20.26					11	11	30.0	21.0
2021-04-22	21.24					8	8	30.0	27.5
2021-05-20	27.90	7	2			4	13	28.0	29.5
2021-06-23	28.24	3	7	1			10	28.4	30.5
2021-07-15	34.89	1	4	2			7	29.0	29.7
2021-08-18	34.83	2	6	2			10	30.0	29.8
2021-09-17	37.40		2	6			8	31.0	29.8
2021-10-20	33.92			4	2		6	29.0	25.5

物种	精子长度/μm	顶体形状	精核长宽比值	线粒体数目		参考文献
织锦巴非蛤(Paphia textile) 近江蛏(Sinonovacula rivularis)	46.04 57.00~58.00	圆锥形保龄球形	1.47 0.36	4 4	“9+2”双联体微管结构 “9+2”双联体微管结构	本文黄瑞等(2011)
钝缀锦蛤(Tapes conspersus)	59.27	倒“V”形	1.27	5	“9+2”双联体微管结构	连昌朋等(2022)
靓巴非蛤(Paphia schnelliana) 施氏獭蛤(Lutraria sieboldii)	50.16 73.21	圆锥形半球形	1.42 1.14	4 5	— —	Chen等(2021) Chen等(2021)
魁蚶(Scapharca broughtoni)	—	圆锥形	0.89	5	“9+2”双联体微管结构	叶婧等(2012)
斧文蛤(Meretrix lamarkii)	45.20~47.70	圆锥形	1.41	5	“9+2”双联体微管结构	董迎辉等(2012)
波纹巴非蛤(Paphia undulata) 红树蚬(Polymesoda erosa)	— —	圆锥形倒“V”形	1.43 4.00	4 4	“9+2”双联体微管结构 “9+2”双联体微管结构	赵志江等(1992) 郭云鹏等(2020)
长牡蛎(Crassostrea gigas)	57.77	圆锥形	1.12	4	“9+2”双联体微管结构	Dong等(2005)
虾夷扇贝(Patinopecten yessoensis)	50.00	倒“V”形	3.00	4~5	“9+2”双联体微管结构	韩厚伟等(2008)

Spermary development, spermatogenesis and sperm ultrastructure of Paphia textile in the Beibu Gulf, Guangxi

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