广西北部湾海域织锦巴非蛤卵巢发育、卵子及卵黄发生的研究

doi:10.11978/2023070

Abstract

Abstract:

In this study, ovarian development, reproductive cycle, egg development and its ultrastructure of Paphia textile in Beibu Gulf of Guangxi were investigated using anatomical observation, ovarian tissue sectioning and transmission electron microscopy techniques. The results showed that P. textile has one reproductive cycle in one year, and the ovarian development of P. textile can be divided into five stages: proliferating stage (May to July), growing stage (July to September), maturing stage (September to November), spawning stage (November to January of the next year), and resting stage (January to April of the next year). According to the relevant characteristics of the egg development and vitellogenesis of P. textile, it can be divided into four main stages: the first stage is the oocytes stage, the second stage is the phase of previtellogenesis oocytes stage, the third stage is the phase of vitellogenesis oocytes stage, and the fourth stage is the phase of postvitellogenic oocytes stages. The phase of vitellogenesis is an important stage of vitellogenesis, during which mitochondria, endoplasmic reticulum, golgiosome, lysosomes and other organelles form yolk granule in different ways. The water temperature in the sea area was closely related to the ovarian development of P. textile and was an important factor affecting the ovarian development of P. textile. P. textile is hermaphroditic, and no hermaphroditism has been found. The results of the study provide providing basic data for reproductive biology, artificial breeding, breeding and germplasm resources protection of P. textile.

Key words: Paphia textile, ovarian development, oogenesis, ultrastructure, histological section observation

XU Bingjie, LIU Yiming, LIAN Changpeng, WU Tao, PAN Ying. Ovarian development, oocyte and yolk production of Paphia textile in Beibu Gulf, Guangxi[J].Journal of Tropical Oceanography, 2024, 43(2): 48-58.

Figures/Tables 6

Fig. 1

Tab. 1

Fig. 2

Tab. 2

The characteristics of oocytes at different stages of P. textile"

时期	细胞特点	细胞器特点	卵黄特点
卵原细胞期	卵原细胞紧靠滤泡壁, 由泡壁上的生殖上皮细胞增殖而来, 形状呈椭圆形或圆形。细胞卵径约为0.94~8.96μm, 细胞间界限不清晰, 但可观察到细胞核。	细胞核内核质聚集, 核仁明显, 细胞在此期整体染色较深。电镜下可观察到卵原细胞的胞质呈颗粒状, 胞质存在较多的囊泡, 线粒体等细胞器较少, 核仁不位于细胞核的中央。	此期未开始卵黄合成。
卵黄合成前期	在滤泡内壁周围。细胞卵径约为9.51~15.43μm, 前期与滤泡壁相贴合, 后期逐渐朝向滤泡中心, 可见卵质有一部分为变窄狭长, 变为滤泡壁相连的卵柄。形状为椭圆形或梨形。细胞核的染色质有聚集成团块的趋势, 同时卵母细胞胞质内存在许多大小、形状不一的囊泡。	细胞核中核质膨胀成泡状, 逐渐透亮, 形成一个生发泡, 卵母细胞胞质中可观察到线粒体等细胞器, 高尔基体复合体开始形成。粗面内质网形成少量囊泡。脂滴的数量开始增多。	此时有少量卵黄粒在合成, 卵黄粒无膜结构, 体积较小, 呈球状。同时胞质周围有大量的糖原颗粒分布。
卵黄合成期	在滤泡周围和靠中央位置, 细胞卵径约15.06~57.20μm, 细胞形态从扁平或不规则逐渐变为梨形或者葡萄状, 卵柄变窄, 与滤泡壁脱离或仅剩一点柄状连接。细胞核偏向一边, 细胞内卵黄粒增多。卵母细胞表面微绒毛发达。卵母细胞胞质内有大量内质网、线粒体等细胞器。	卵母细胞中细胞器数量多。内质网发达, 分为层状和环状结构, 环状结构还会包围脂滴进行卵黄粒的合成。高尔基体复合体在边缘形成囊泡, 包囊包裹线粒体等进行卵黄粒沉淀。	此时卵黄大量合成, 卵黄的来源多种, 形状与大小不一, 不同来源的卵黄粒沉积程度有差别。
卵黄合成后期	成熟卵子形状为球形, 卵子间相互挤压, 分布在滤泡中央位置。卵径比大生长期的卵母细胞稍大一些, 细胞卵径约为 48.78~62.54μm, 呈饱满状, 核膜清晰, 核仁靠在细胞核的一边、卵黄粒多, 大量分布在核质中。胞质内充满卵黄粒, 卵黄饱满, 成熟卵细胞相互挤压, 细胞核清晰、有少部分可观察到存在2个核仁。	卵母细胞的细胞器数量大幅减少。内质网和高尔基体复合体等细胞器特征已经不明显, 形成包囊。Ⅱ型线粒体内部脊消失, 出现空泡化, 合成卵黄粒。	大量卵黄分布在成熟卵子内, 多为球形, 大部分发育成熟。卵黄粒在卵母细胞内呈现饱满状, 数量多、体积大。

Tab. 2

Fig. 3

Fig. 4

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取样日期/(年-月-日)	卵巢发育各期内的个体数					总数/个	盐度/‰	水温/℃
取样日期/(年-月-日)	Ⅰ	Ⅱ	Ⅲ	Ⅳ	Ⅴ	总数/个	盐度/‰	水温/℃
2020-11-29			2	4		6	27.4	22.0
2020-12-21			5	6		11	27.5	17.2
2021-01-08			2	9	1	12	29.4	15.6
2021-02-24				2	8	10	30.9	17.3
2021-03-18				1	8	9	30.0	21.0
2021-04-22					12	12	30.0	27.5
2021-05-20	2	1			4	7	28.0	29.5
2021-06-23	2	4			4	10	28.4	30.5
2021-07-15	5	6	2			13	29.0	29.7
2021-08-18	3	6	1			10	30.0	29.8
2021-09-17	2	3	5	2		12	31.0	29.8
2021-10-20			13	1		14	29.0	25.5

Ovarian development, oocyte and yolk production of Paphia textile in Beibu Gulf, Guangxi

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[2]	WU Tao, PAN Ying, LIU Yiming, LIAN Changpeng, XU Bingjie, WANG Chaoqi, YANG Ling. Testis development, spermatogenesis and sperm ultrastructure of Lutraria sieboldii in the Beibu Gulf, Guangxi [J]. Journal of Tropical Oceanography, 2024, 43(2): 69-80.
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[4]	LIAN Changpeng, WU Tao, WANG Chaoqi, YANG Ling, PAN Ying. Ovarian development, histology of oogenesis and yolk formation of Tapes conspersus from the Beihai Yingpan, Guangxi [J]. Journal of Tropical Oceanography, 2022, 41(5): 170-179.
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[6]	Huan ZHOU, Gang LIN, Xiaozhen RAO. Ultrastructure of spermatogenesis and spermatozoon of Megabalanus volcano [J]. Journal of Tropical Oceanography, 2020, 39(3): 98-105.
[7]	SONG Yuefan, QU Yi, CAO Xupeng, WANG Qiukuan, ZHANG Wei. The tissue structure and cellular characteristics of marine sponge Axinella sp. living in the South China Sea [J]. Journal of Tropical Oceanography, 2016, 35(4): 71-81.
[8]	WU Ming-can, ZHANG Li, PAN Ying, HUANG Guang-hua, Li Yong-mei, YANG Xue-ming. Ultrastructure of oogenesis and vitellogenesis of sea cucumber Holothuria scabra [J]. Journal of Tropical Oceanography, 2015, 34(3): 68-74.
[9]	NI Na, LIU Xue-zhou, XU Yong-jiang, ZHAO Ming, QU Jian-zhong. Histological studies on the pituitary gland of female barfin flounder Verasper moseri [J]. Journal of Tropical Oceanography, 2012, 31(6): 97-102.
[10]	HUANG Kai-hsiang,WU Jui-pin,CHEN Hon-cheng. Ovarian development and reproductive cycle in female freshwater prawn Macrobrachium asperulum [J]. Journal of Tropical Oceanography, 2011, 30(1): 159-164.
[11]	CAO Fu-jun,LUO Jie,LI Chang-ling,LIU Chu-wu. Study on the reproductive system of Hemifusus ternatanus [J]. Journal of Tropical Oceanography, 2010, 29(6): 57-64.
[12]	DAI Yu-lai,Lü Yong-lin,LI Kai,TAO Zhen,WANG Guo-liang. Histopathological and ultrapathological study on ulcer disease of Pseudosciaena crocea [J]. Journal of Tropical Oceanography, 2010, 29(5): 174-179.
[13]	XU You-hou,LIU Xue-dong,ZHANG Lü-ping,HU Chao-qun. Studies on the ultrastructure of spermatogenesis in shrimp, Litopenaeus vannamei [J]. Journal of Tropical Oceanography, 2010, 29(4): 89-93.
[14]	ZHANG Dian-cai,RAO Xiao-zhen,LIN Gang,XU You-qin,CHEN Yin-shan. Ultrastructure of spermatogenesis and mature sperm of Solen grandis [J]. Journal of Tropical Oceanography, 2009, 28(6): 131-135.