Journal of Tropical Oceanography ›› 2024, Vol. 43 ›› Issue (1): 154-166.doi: 10.11978/2023045CSTR: 32234.14.2023045

• Exploitation of Marine Resources • Previous Articles    

Study on the chemical constituents and pharmacological activity of the marine-derived fungus Aspergillus fumigatus DL-p0m-g2 in the Beibu Gulf

FENG Ting(), SUN Jian, WANG Yufei, PAN Weibin, QIN Xucan, QIN Bingyun, ZHOU Liman, WANG Cong, WANG Pei, KONG Fandong()   

  1. School of Chemistry and Chemical Engineering, Key Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products, Key Laboratory of Universities in Guangxi for Excavation and Development of Ancient Ethnomedicinal Recipes, Guangxi Minzu University, Nanning 530006, China
  • Received:2023-04-08 Revised:2023-04-18 Online:2024-01-10 Published:2024-01-19
  • Supported by:
    National Natural Science Foundation of Guangxi Province(2021GXNSFBA075036); Specific Research Project of Guangxi for Research Bases and Talents(AD22035018); National Natural Science Foundation of China(82104034); Xiangsi Lake Youth Innovation Team Project of Guangxi Minzu University(2021RSCXSHQN01); 2021 University-Level Scientific Research Projects of Guangxi Minzu University(2021 MDKJ003)

Abstract:

The chemical constituents and their bioactivities of Aspergillus fumigatus DL-p0m-g2 from the Beibu Gulf were studied in order to obtain bioactive compounds. The compounds were isolated and purified by reversed-phase ODS column chromatography, semi-preparative liquid chromatography and other chromatographic methods. Their structures were identified by physicochemical properties, mass spectrometry and nuclear magnetic resonance spectroscopy. The cytotoxic, antibacterial and cholesterol transporter NPC1L1 protein binding activities of the isolated compounds were evaluated. As a result, a total of 21 alkaloids and one steroid were isolated and identified as 6-methoxyspirotryprostatin B (1), spirotryprostatin A (2), fumitremorgin C (3), cyclotryprostatin A (4), fumitremorgin B (5), pseurotin A (6), azaspirofuran A (7), azaspirofuran B (8), cephalimysin C (9), cephalimysin B (10), fumiquinazoline C (11), fumiquinazoline B (12), fumiquinazoline A (13), fumiquinazoline D (14), fumiquinazoline F (15), tryprostatin B (16), verruculogen (17), chaetominine (18), bisdethiobis(methylthio)glitoxin (19), helvolic acid (20), 7-deacetylpyripyropene A (21), terezine D (22). Compound 6 showed moderate cytotoxic activity against human hepatoma cells (HepG2), human lung cancer cells (A549) and human rectal cancer cells (HCT116). Compounds 1, 3 and 20 showed antibacterial activity against Staphylococcus aureus. Compound 14 showed a good binding with NPC1L1 protein, indicating its potential in the development of lipid-lowering drugs.

Key words: Aspergillus fumigatus, chemical constituents, cytotoxic activity, anti-bacteria activity, NPC1L1 protein binding