红树老鼠簕内生真菌Aspergillus terreus GXIMD 03158次级代谢产物研究

doi:10.11978/2023158

Abstract

Abstract:

This work studies the secondary metabolites with antibacterial activity from Aspergillus terreus GXIMD 03158, an endophytic fungus isolated from mangroves Acanthus ilicifolius L. The secondary metabolites of the strains were separated and purified by various chromatographic techniques, and the structures of monomer compounds were identified by comprehensive analyses of ¹H, ¹³C NMR and HREIMS date. Ten compounds were isolated from the metabolites of A. terreus GXIMD 03158, and identified as dankasterones A (1), (14α, 22E)-14-hydroxyergosta-4, 7, 22-triene-3, 6-dione (2), steresterone B (3), ergosta-4, 6, 8(14), 22-tetraen-3-one (4), herbarulide (5), demethylincisterol A₃ (6), isochaetochromin B₁ (7), isochaetochromin B₂ (8), (E)-7, 9-diene-11-carbonyl stearic acid (9) and aspergilfuranone A (10). The minimal inhibitory concentrations (MIC) of all compounds against Staphylococcus albus, Staphylococcus aureus, Staphylococcus epidermidis, Methicillin-resistant Staphylococcus aureus and Vibrio parahaemolyticus were determined by broth microdilution method, the results showed that compound 6 exhibited significant antibacterial activity against S. albus, S. aureus and S. epidermidis, and the MIC values were 3.12, 6.25 and 3.12 μg·mL^-1, respectively; compound 9 exhibited significant antibacterial activity against S. albus and S. aureus, and the MIC values were 1.56 and 3.12 μg·mL^-1, respectively. This study provides a theoretical basis for the research on development and utilization of mangrove Acanthus ilicifolius L. and its endophytic fungi.

Key words: mangroves Acanthus ilicifolius L., endophytic fungi, Aspergillus terreus, secondary metabolites, antibacterial activities

AN Fan, JIANG Yue, WANG Yu, CAO Guangping, GAO Chenghai, LIU Yonghong, YI Xiangxi, BAI Meng. Studies on secondary metabolites of endophytic fungus Aspergillus terreus GXIMD 03158 isolated from mangroves Acanthus ilicifolius L.[J].Journal of Tropical Oceanography, 2024, 43(5): 41-48.

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References 27

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Studies on secondary metabolites of endophytic fungus Aspergillus terreus GXIMD 03158 isolated from mangroves Acanthus ilicifolius L.

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