Abstract: In order to obtain compounds with anti-aging activity in the fruit of Sonneratia apetala, the chemical constituents of n-butanol alcohol extract were separated and their bioactivities were studied. Compounds were isolated and purified by reverse silica column, gel chromatography and high pressure liquid chromatography. The structure of compounds was identified by modern spectral analysis and literature contrast. The anti-aging activity of compounds were investigated based on Caenorhabditis elegans used the lifespan, heat stress, pharyngeal pumping capacity, body bending ability and lipofuscin as indicator. Molecular docking was used in predicted the interacted mode of compounds with protein DAF-16. Five compounds were isolated from the n-butanol alcohol extract for the first time, identified as 1, 2, 3, 6-tetra-O-galloyl -β-D-glucopyranose (1), hovetrichoside C (2), (6R, 9S)-3-oxo-α-ionol-β-D-glucoside (3), kaempferol-3-O-β-D glucoside (4), 3-methylbutyl β-D-glucopyranoside (5). Compounds 2, 4 and 5 showed an anti-aging activity at 100μmol·L-1 concentration significantly, extended the lifespan of C. elegans in normal culture by 15.9%, 15.0% and 22.1% respectively, significantly prolong the lifespan under heat stress by 36.3%, 50.6% and 46.3% respectively. Improved the frequency of pharyngeal pumping, ability of body bending, and reduced lipofuscin accumulated. Molecular docking showed that compounds 2, 4 and 5 can stably bind to the amino acids of DAF‐16 protein. The binding energy were -6.6kcal·moL-1, -6.0kcal·moL-1, -4.6kcal·moL-1 respectively. This study enriched the material basis of anti-aging activity and provided a basis for the development and utilization in the Sonneratia apetala fruit.

Key words: sonneratia apetala, glycoside compounds, caenorhabditis elegans, anti-aging