Khai岛和Pathiu岛珊瑚礁沉积物细菌多样性及细菌粗提物延缓秀丽隐杆线虫衰老活性研究*

doi:10.11978/2019126

Abstract

Abstract:

Two coral reef sediment samples were collected from the coral reef growth areas of Khai Island and Pathiu Island After the samples were pretreated, six kinds of separation medium were adopted to obtain the active Marine bacterial strains. Through PCR amplification and 16s rRNA gene sequencing, species diversity was analyzed and phylogenetic trees were constructed. The effect of fermentation extract of Caenorhabditis elegans strain on senescence was investigated. The results showed that 22 strains of bacteria were isolated and identified from four samples, belonging to 11 orders, 13 families and 14 genera. There were two strains of bacteria with 16S rRNA gene sequence similarity less than 98.65 %, which were suspected to be potential new species. The crude extracts of 500 μg·mL ^-1bacteria from GXIMD014 (Vibrio galatheae) and GXIMD112 (Kocuria kristinae) have the activity of delaying the aging of Caenorhabditis elegans, and can prolong the life of Caenorhabditis elegans to different degrees, improve the oviposition, motility, heat-induced injury, ability to resist oxidative damage, enzyme activity in vivo, and improve the epidermal morphology of Caenorhabditis elegans. Coral reef sediment bacteria of Khai Island and Pathiu Island have high species diversity and rich microbial and bacterial resources, and some bacterial strains have anti-aging biological activity.

Key words: coral reef, marine bacteria, species diversity, anti-aging activity

CLC Number:

P735.51

HAN Minmin, LI Mi, LIU Xinming, LIU Yonghong, LONG Chao, ZHONG Zhenguo, YI Xiangxi, GAO Chenghai. Studies on bacterial diversity in coral reef sediments in Khai Island and Pathiu Island and bacterial crude extract retards aging activity of Caenorhabditis elegans*[J].Journal of Tropical Oceanography, 2020, 39(5): 19-29.

Figures/Tables 13

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References 16

[1]	蔡雪芹, 2018. 浅谈珊瑚及其生态功能与保护[J]. 海洋与渔业, (7):100-102 (in Chinese).
[2]	李菲, 高程海, 余炼, 等, 2018. 川蔓藻内生及根际细菌多样性与抑菌活性研究[J]. 广西植物, 38(7):922-933.
	LI FEI, GAO CHENGHAI, YU LIAN, et al, 2018. Diversity and antifungal activity of endophytic and rhizospheric bacteria isolated from Ruppia maritima[J]. Guihaia, 38(7):922-933 (in Chinese with English abstract).
[3]	孙静, 王素英, 张德超, 等, 2014. 海南红树林根系土壤中可培养细菌的多样性分析[J]. 海洋科学, 38(7):27-33.
	SUN JING, WANG SUYING, ZHANG DECHAO, et al, 2014. Diversity of culturable bacteria from the soil of root system of mangrove forest of Beigang island in Hainan Province[J]. Marine Sciences, 38(7):27-33 (in Chinese with English abstract).
[4]	王怀玲, 2018. 蓝莓多酚化合物抗衰老活性及作用机制研究[D]. 广州: 华南理工大学: 1-172.
	WANG HUAILING, 2018. Research on Antiaging activity and mechanism of blueberry polyphenols[D]. Guangzhou: South China University of Technology: 1-172 (in Chinese with English abstract).
[5]	周双清, 黄小龙, 黄东益, 等, 2010. Chelex-100快速提取放线菌DNA作为PCR扩增模板[J]. 生物技术通报, 22(2):123-125.
	ZHOU SHUANGQIN, HUANG XIAOLONG, HUANG DONGYI, et al, 2010. A rapid method for extracting DNA from actinomycetes by chelex-100[J]. Biotechnology Bulletin, 22(2):123-125 (in Chinese with English abstract).
[6]	BRENNER S, 1974. The genetics of Caenorhabditis elegans[J]. Genetics, 77(1):71-94. pmid: 4366476
[7]	CHISHOLM A D, XU SUHONG, 2012. The Caenorhabditis elegans epidermis as a model skin. II: Differentiation and physiological roles[J]. Wiley Interdisciplinary Reviews: Developmental Biology, 1(6):879-902. doi: 10.1002/wdev.77 pmid: 23539358
[8]	HARTMANN A C, PETRAS D, QUINN R A, et al, 2017. Meta-mass shift chemical profiling of metabolomes from coral reefs[J]. Proceedings of the National Academy of Sciences of the United States of America, 114(44):11685-11690. doi: 10.1073/pnas.1710248114 pmid: 29078340
[9]	HERNDON L A, SCHMEISSNER P J, DUDARONEK J M, et al, 2002. Stochastic and genetic factors influence tissue-specific decline in ageing C. elegans[J]. Nature, 419(6909):808-814. pmid: 12397350
[10]	KIM K H, ROH S W, CHANG HW, et al, 2009. Nitratireductor basaltis sp. nov., isolated from black beach sand[J]. International Journal of Systematic and Evolutionary Microbiology, 59(1):135-138.
[11]	KIM M, OH H S, PARK S C, et al, 2014. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes[J]. International Journal of Systematic and Evolutionary Microbiology, 64(2):346-351.
[12]	LUEPONGPATTANA S, THANIYAVARN J, MORIKAWA M, 2017. Production of Massoia Lactone by Aureobasidium pullulans YTP6-14 isolated from the gulf of Thailand and its fragrant biosurfactant properties[J]. Journal of Applied Microbiology, 123(6):1488-1497. doi: 10.1111/jam.13598 pmid: 28972680
[13]	PARK H E H, JUNG Y, LEE S J V, 2017. Survival assays using Caenorhabditis elegans[J]. Molecules and Cells, 40(2):90-99. pmid: 28241407
[14]	SUGI T, 2016. Genome editing in C. elegans and other nematode species[J]. International Journal of Molecular Sciences, 17(3):295. doi: 10.3390/ijms17030295 pmid: 26927083
[15]	SUPONG K, SURIYACHADKUN C, PITTAYAKHAJONWUT P, et al, 2013. Micromonospora spongicola sp. nov., an actinomycete isolated from a marine sponge in the Gulf of Thailand[J]. The Journal of Antibiotics, 66(9):505-509. pmid: 23677031
[16]	THAWORNWIRIYANUN P, TANASUPAWAT S, DECHSAKUL WATANAC, et al, 2012. Identification of Newly Zeaxanthin- producing bacteria isolated from sponges in the gulf of Thailand and their Zeaxanthin production[J]. Applied Biochemistry and Biotechnology, 167(8):2357-2368. doi: 10.1007/s12010-012-9760-2 pmid: 22715027

类群	菌株编号	相似性最高模式菌株	16S rRNA基因序列相似性/%	GenBank登录号
Kocuria sp.	GXIMD112	K. Kristinae (BCSM01000028)	99.74	MT111730
Vibrio sp.	GXIMD014	V. Galatheae (JXXV01000023)	99.35	MT111742
Vibrio sp.	GXIMD099	V. Sagamiensis (AB428909)	98.75
Bacillus sp.	GXIMD115	B. Megaterium (JJMH01000057)	99.74
	GXIMD039	B. Aryabhattai (EF114313)	99.48
	GXIMD105	B. Altitudinis (ASJC01000029)	98.97
Pseudomonas sp.	GXIMD091	P. Pachastrellae (MUBC01000081)	98.97
Shewanel sp.	GXIMD013	S.Waksmanii (AY170366)	99.59
Staphylococcus sp.	GXIMD124	S. Gallinarum (D83366)	99.23
	GXIMD017	S. Nepalensis (UHDS01000001)	99.09
	GXIMD114	S. Haemolyticus (LILF01000056)	100.00
	GXIMD133	S. hominis subsp. Novobiosepticus (AB233326)	99.35
Achromobacter sp.	GXIMD088	A. marplatensis (EU150134)	98.83
Achromobacter sp.	GXIMD134	A. Ruhlandii (AB010840)	99.61
Leeuwenhoekiella sp.	GXIMD126	L. Palythoae (jgi.1059018)	98.83
Marinomonas sp.	GXIMD067	M. Vaga (X67025)	99.09
Gordonia sp.	GXIMD145-1	G. Bronchialis (CP001802)	99.23
Rhizobium sp.	GXIMD055	R. Roseltifomans (EU781656)	99.36
Erythrobacter sp.	GXIMD154	E. Pelagi (HQ203045)	99.87
Alteromonas sp.	GXIMD063	A. Macleodii (CP003841)	99.61
Alteromonas sp.	GXIMD003	A. Marina (AF529060)	97.93
Aestuariibacter sp.	GXIMD144	A. aggregatus (FJ847832)	96.68

菌株编号	平均寿命/d	最长寿命/d	菌株编号	平均寿命/d	最长寿命/d
1% DMSO	16.68±1.057	18.83	GXIMD039	19.75±1.060*	21.89
GXIMD055	14.50±0.844	16.21	GXIMD114	15.30±0.834	16.99
GXIMD099	15.50±0.938	17.40	GXIMD115	14.25±0.839	15.95
GXIMD154	16.56±1.126	18.84	GXIMD091	17.10±1.261	19.65
GXIMD144	14.30±0.818	15.96	GXIMD088	15.65±0.826	17.32
GXIMD112	20.75±1.417**	23.62	GXIMD145-1	14.27±0.891	16.08
GXIMD013	16.20±0.891	18.00	GXIMD014	21.75±1.119**	22.01
GXIMD124	16.45±1.041	18.56	GXIMD003	15.05±0.950	16.97
GXIMD017	17.22±1.250	19.78	GXIMD134	16.10±1.107	18.34
GXIMD126	15.20±0.925	17.07	GXIMD063	13.25±0.867	15.00
GXIMD067	13.15±0.671	14.51	GXIMD133	14.40±0.817	16.05
GXIMD105	14.22±1.010	16.26

属	种	菌株粗提物	菌株粗提物编号	浓度/(μg·mL^-1)	菌株粗提物编号
Vibrio sp.	Vibrio galatheae	GXIMD014细菌粗提物	S1	250	S1-1
				500	S1-2
				1000	S1-3
Kocuria sp.	Kocuria kristinae	GXIMD112细菌粗提物	S2	250	S2-1
				500	S2-2
				1000	S2-3
Bacillus sp.	Bacillus aryabhattai	GXIMD039细菌粗提物	S3	250	S3-1
				500	S3-2
				1000	S3-3

Studies on bacterial diversity in coral reef sediments in Khai Island and Pathiu Island and bacterial crude extract retards aging activity of *Caenorhabditis elegans**

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Studies on bacterial diversity in coral reef sediments in Khai Island and Pathiu Island and bacterial crude extract retards aging activity of Caenorhabditis elegans*

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Studies on bacterial diversity in coral reef sediments in Khai Island and Pathiu Island and bacterial crude extract retards aging activity of *Caenorhabditis elegans**