中国石莼属海藻新记录种——大野石莼(Ulva ohnoi)的形态与多基因标记分析

doi:10.11978/2025050

Abstract

Abstract:

This study presents a newly recorded species of the Ulva algae in China, Ulva ohnoi Hiraoka & S. Shimada 2004, collected from Fangchenggang (Guangxi) and Zhanjiang (Guangdong). Detailed morphological characteristics were described, accompanied by a multi-gene marker analysis. The results indicate that Ulva ohnoi exhibits both attached and floating forms. The thalli are light green, 15-60 cm in length, solitary or tufted. The tissue is brittle and easily torn, displaying round, ovate, or irregular shapes. The thallus surface cells are square or polygonal, arranged irregularly, with jagged protrusions along the edges. The upper and middle parts of the thallus are similar, with cell dimensions of 10-20 μm×5-15 µm and a thickness of 20-45 µm. In contrast, the basal cells are nearly round, measuring 10-30 μm×10-22 µm, with a thickness of 70-90 µm. Analysis of the rbcL and tufA gene sequences revealed that the samples collected in this study are genetically identical to the type specimens of Ulva ohnoi, supported by high bootstrap values and posterior probabilities. This study enriches the species diversity of the Ulva genus in China, underscores the significance of molecular biology techniques in identifying green algae species, and provides essential taxonomic data for the conservation and utilization of marine algae resources in China.

Key words: Ulva ohnoi, newly recorded species, morphology, multi-gene markers, rbcL, tufA

CLC Number:

Q949.21

ZENG Zhaojun, XIE Enyi, CUI Jianjun. A newly recorded species of Ulva in China: morphological and multi-gene marker analysis of Ulva ohnoi[J].Journal of Tropical Oceanography, 2025, 44(6): 64-73.

Figures/Tables 7

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基因区域	引物名称及序列	参考文献
rbcL	RH1 (5′-ATGTCACCACAAACAGAAACTAAAGC-3′) 1385r (5′-AATTC AAATTTAATTTCTTTCC-3′)	Manhart, 1994
tufA	tufGF4 (5′-GGNGCNGC NCAAATGGAYGG-3′) tufAR (5′-CCTTCNCGAATMGCRAAWCGC-3′)	Famà et al, 2002

基因区域	预变性	变性	退火	延伸	循环数	最终延伸
rbcL	95℃ 6min	95℃ 1min	45℃ 1min	72℃ 1min	35	72℃ 10min
tufA	94℃ 4min	95℃ 1min	45℃ 30s	72℃ 1min	35	72℃ 7min

样本编码	采集地点	采集日期	rbcL基因序列号	tufA基因序列号
FCG001	广西防城港	2025-3-1	PV282387	/
FCG002	广西防城港	2025-3-1	PV282388	PV366677
FCG003	广西防城港	2025-3-1	PV282389	PV366678
FCG004	广西防城港	2025-3-1	/	/
ZJ001	广东湛江	2024-3-25	PV282390	/
ZJ002	广东湛江	2025-3-4	PV282391	PV366679
ZJ003	广东湛江	2025-3-4	/	PV366680
ZJ004	广东湛江	2025-3-4	/	PV366681
ZJ005	广东湛江	2025-3-4	/	PV366682
ZJ006	广东湛江	2025-3-4	/	PV366683
注: “/”表示序列未扩增成功。

A newly recorded species of Ulva in China: morphological and multi-gene marker analysis of Ulva ohnoi

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