Journal of Tropical Oceanography ›› 2009, Vol. 28 ›› Issue (6): 72-78.doi: 10.11978/j.issn.1009-5470.2009.06.072cstr: 32234.14.j.issn.1009-5470.2009.06.072

• Marine biology • Previous Articles     Next Articles

Genetic analysis of the marine mussel Perna viridis (Bivalvia: Mytilidae) based on mitochondrial COI sequence and its phylogenetic relationship with other congeneric species

WEI Zheng-peng 1,KONG Xiao-yu1, 2 ,WU Xiang-yun2,YU Zi-niu2   

  1. 1.Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China; 2.South China Sea Institute of Oceanology, Chinese Academy of Sciences, Laboratory of Applied Marine Biology,Guangzhou 510301, China
  • Received:2007-11-09 Revised:2008-05-04 Online:2009-12-30 Published:2009-12-12
  • Contact: 喻子牛 E-mail: carlzyu@scsio.ac.cn
  • About author:位正鹏(1982),男,山东省莱阳市人,硕士研究生,主要从事海洋动物分子生物学研究;E-mail: weizhengpeng1234@yahoo.com.cn
  • Supported by:

    国家自然科学基金项目(30570242);国家“863”计划项目(2006AA10A409)

Abstract:

Partial mitochondrial COI gene sequences from the gonads (male and female) and somatic tissue of mussel Perna viridis were separately amplified by PCR using the universal primers COIL 1490/COIH 2198. A 661 bp nucleotide sequence of COI gene was obtained. It was found that this gene sequence was identical among different tissues of male individual, and low divergence (0.31%) of COI sequence was detected between male and female of the gonad tissues. Only one haplotype was found in both female and male for the sequence. No doubly uniparental inheritance (DUI) was detected. Based on the COI sequences obtained from this study and those of other Perna species from GenBank, NJ tree, MP tree and Bayesian inference (BI) tree were constructed with PAUP and MrBayes respectively, and phylogenetic relationships among them were analyzed accordingly.

Key words: mitochondrial DNA, COI gene, Perna viridis, doubly uniparental inheritance (DUI)