激素诱导花鳗鲡精巢发育成熟期间性激素水平及相关细胞的超显微结构

doi:10.11978/j.issn.1009-5470.2010.06.130

Journal of Tropical Oceanography ›› 2010, Vol. 29 ›› Issue (6): 130-136.doi: 10.11978/j.issn.1009-5470.2010.06.130cstr: 32234.14.j.issn.1009-5470.2010.06.130

• Marine biology • Previous Articles Next Articles

Variations in sex steroid hormone level and changes in ultrastructure of related cells during the artificial maturation of spermary of Anguilla marmorata

QI Xin1, HUANG Hai1,2, ZHOU Wen-yi1, YIN Shao-wu1, ZHANG Yong2, LIU Xiao-chun2, CHEN Guo-hua1, LIN Hao-ran1,2

1. College of Ocean, Hainan University, Key Laboratory of Tropical Biology Resources, Ministry of Education, Haikou 570228, China; 2. Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University, Guangzhou 510275, China

Received:2009-01-22 Revised:2009-11-03 Online:2010-12-15 Published:2010-12-15
Contact: 林浩然。 E-mail:lsslhr@mail.sysu.edu.cn
About author:齐鑫(1983—), 男, 辽宁省沈阳市人, 硕士研究生, 主要从事鱼类生理学研究。E-mail: forever_qixin@yahoo.com.cn
Supported by:
国家自然基金面上项目(30770283); “十一五”国家科技支撑计划重点项目(2007BAD29B03); 海南省教育厅高等学校科学研
究资助项目(Hjkj2008-22); 广东省科技兴海(渔)项目(B200701A06)

Abstract

Abstract:

Serum levels of gonadotropin (GtH), testosterone (T), 11-ketotestosterone (11-KT), and estradiol-17β (E2) were assessed by radioimmunoassay during induction of sexual maturation of male marbled eel (Anguilla marmorata) by Human Chorionic Gonadotropin (HCG) treatment. Morphological alterations of the liver and GtH cell of Anguilla marmorata were investigated by means of light and electron microscopy. The testis Gonadosome Index (GSI) was 0.17±0.06% in the control group and their serum GtH and 11-KT levels were low, the T level was lower than 0.1 ng (the range of kit was 0.1―20ng•mL?1); Serum GtH, T, 11-KT levels increased rapidly in the operated group after the third injection of HCG, when the GSI of testis was 7.0±0.03%. After the spermiation, the GtH, T and 11-KT levels decreased a little, but still higher than the levels of the control group. Serum E2 levels decreased gradually as HCG treatment progressed, from 2.36 ng/ml of the control group to 0.83 ng/ml of the spermiation eels. In the hepat of the control group, abundant glycongen and little lipid droplets were obversived, the nuclear was in the middle of the cell. In contrast, in the hepat of the spermiation eel, the lipid droplets accu-mulated, which lead to the closing of nuclear to the cell membrane.

Key words: Anguilla marmorata, artifical reproduction, sex steroid hormone, gonadotropin, sperm, GtH cell, hepatic cells

QI Xin,HUANG Hai,ZHOU Wen-yi,YIN Shao-wu,ZHANG Yong,LIU Xiao-chun,. Variations in sex steroid hormone level and changes in ultrastructure of related cells during the artificial maturation of spermary of Anguilla marmorata[J].Journal of Tropical Oceanography, 2010, 29(6): 130-136.

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Variations in sex steroid hormone level and changes in ultrastructure of related cells during the artificial maturation of spermary of Anguilla marmorata

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