一株微微型浮游植物的分离培养与鉴定

doi:10.11978/j.issn.1009-5470.2011.01.086

Journal of Tropical Oceanography ›› 2011, Vol. 30 ›› Issue (1): 86-90.doi: 10.11978/j.issn.1009-5470.2011.01.086cstr: 32234.14.j.issn.1009-5470.2011.01.086

• Marine biology • Previous Articles Next Articles

Isolation, culture, and identification of a picophytoplankton

ZUO Yun-long1,2, XIANG Wen-zhou1, LIU Ji-hua1,2, SU Jiao-jiao1,2, HE Hui1, ZHANG Li1, XIAO Wei1

1. South China Sea institute of Oceanology, CAS, Guangzhou 510301, China; 2. Graduate University of CAS, Beijing 100039, China

Received:2009-01-16 Revised:2010-05-14 Online:2011-03-17 Published:2011-03-16
Contact: 向文洲。E-mail: xwz@scsio.ac.cn
About author:左云龙(1982—), 男, 山东省郯城县人, 硕士研究生, 从事微藻生物技术研究。
Supported by:
广东省科技计划项目(2009B080701093); 广东省海洋与渔业局科技专项项目(A2008009-08)

Abstract

Abstract:

In this study, strain SCSIO-8, an isolated picophytoplankton was obtained from the continental shelf of the northern South China Sea. Studies were carried out on its growth, morphological structure, physiological and biochemical characteris-tics and taxonomic identification. The results indicate that SCSIO-8 was a free unicellular alga without flagellate, (2.08±0.28)×(1.78±0.09)µm in size, and no pyrenoid, 1 chloroplast, 1 mitochondria, 1 nucleus, and many starch granules were observed in its cell. It was revealed that SCSIO-8 reproduced through autosporation. Results also indicate that SCSIO-8 was able to grow in a wide range of salinity from 0 to 38‰ at least, and adapted rapidly to the mutual switch between seawater and freshwater. HPLC chromatogram analysis showed that the main photosynthetic pigments of SCSIO-8 were chlorophyll a, chlorophyll b, and several carotenoids including cis- and trans-Violaxanthin, Lutein, β-carotene, etc.18S rDNA region of SCSIO-8 genome DNA was sequenced and blasted in GenBank. The picophytoplankton strain could be identified as Pi-cochlorum sp. SCSIO-8 through integrated analysis of its morphological, physiological, biochemical, and molecular charac-teristics.

Key words: picophytoplankton, Picochlorum, taxonomic identification, 18S rDNA

ZUO Yun-long,XIANG Wen-zhou,LIU Ji-hua,SU Jiao-jiao,HE Hui,ZHANG. Isolation, culture, and identification of a picophytoplankton[J].Journal of Tropical Oceanography, 2011, 30(1): 86-90.

References

[1] SIEBURTH, MCN J, SMETACEK V, et al. Pelagic ecosystem structure: Heterotrophic compartments of the plankton and their relationship to plankton size fraction[J]. Limnology and Oceanography, 1978, 23: 1256-1263.

[2] 陈超群, 陈世杰. 浮游植物初级生产理论的新进展[J].福建水产, 1995, 1: 78-80.

[3] RAVEN J A. Physiological consequences of extremely small size for autotrophic organisms in the sea[J].Can Bull. Fish Aquat Sci, 1986, 214: 1-70.

[4] ZEVENBOOM W. Ecophysiology of nutrient uptake, photosynthesis and growth[J].Can J Fish Aquat Sci, l986, 214: 391-422.

[5] ZUBKOV M, FUCHS B M, TARRAN G A, et al.High rateof uptake of organic nitrogen compounds by Prochlorococcus cyanobacteria as a key to their dominancein oligotrophic oceanic waters[J]. Applied and Environmental Microbiology, 2003, 69(2): 1299-1304.

[6] 孙晓庆, 董树刚. 微微型浮游植物的生态学研究进展[J]. 海洋科学, 2008, 32(5): 67-72.

[7] EVELYNE DERELLEA, CONCHITA FERRAZ B, STEPHANE ROMBAUTS, et al. Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features[J]. PNAS, 2006, 103(31): 11647–11652.

[8] 钟瑜, 黄良民. 海洋微型和微微型浮游生物的区域分布与影响因素[J]. 生态科学, 2008, 27(3): 179-185

[9] 汪岷, 白晓歌, 梁彦韬, 等. 北黄海夏季微微型浮游植物的分布[J]. 植物生态学报, 2008, 32(5): 1184-1193.

[10] HIROSE M, KATANO T, SHIN-ICHI NAKANO. Growth and grazing mortality rates of Prochlorococcus, Synechococcus and eukaryotic picophytoplankton in a bay of the Uwa Sea, Japan[J]. Journal of Plankton Research, 2008, 30(3): 241-250.

[11] LE GALL F, RIGAUT-JALABERT F, MARIE D, et al. Picoplankton diversity in the South-East Pacific Ocean from cultures[J]. Biogeosciences, 2008, 5: 203-214.

[12] YAMAMOTO M, NOZAKI H, MIYAZAWA Y. Relationship between presence of a mother cell wall and speciation in the unicellular microalga Nannochloris (Chlorophyta)[J]. Journal of Phycology, 2003, 39(1): 172-184.

[13] HENLEY W J, HIRONAKA J L, GUILLOU L, et al. Phylogenetic analysis of the Nannochloris―like algae and diagnoses of Picochlorum oklahomensis gen. et sp. nov. (Trebouxiophyceae, Chlorophyta)[J]. Phycologia, 2004,43(6): 641-652.

[14] YUAN J P, CHEN F. Chromatographic separation and purification of trans-astaxanthin from the extracts of Haematococcus pluvialis[J]. J Agric Food Chem, 1998, 46(8): 3371-3375.

[15] POLNE-FULLER M．A two—hour method for extraction of DNA from seaweeds[J]. Phycological Newsletter, 1991, 27(3): 11.

[16] GEISERT M, ROSE T, BAUER W, et al. Occurrence of carotenoids and sporopollenin in Nanochlorum eucaryotum, a novel marine alga with unusual unusual characterstics[J]. Biosystems, 1987, 20(2): 133-142.

[17] WILHELM C, EISENBEIS G, WILD A, et al. Nanochlorum eucaryotum: a very reduced coccoid species of marine Chlorophyceae[J]. Z Naturforsch, 1982, 37c: 107-114.

Isolation, culture, and identification of a picophytoplankton

PDF (PC)

Like

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 2

Metrics

Comments

Recommended 0

[1]	LIN Xianzhi, ZHOU Yanyan, LIN Haoye, HU Simin, HUANG Hui, ZHANG Li, LIU Sheng. Diet analysis of the parrotfish (Scarus globiceps) in coral reefs of the Nansha Islands [J]. Journal of Tropical Oceanography, 2024, 43(3): 100-108.
[2]	JIANG Hai-ying, LUO Peng, YU Zong-he, PENG Peng-fei, HU Chao-qun. Identification of Enteromorpha sp. from Daya Bay and its capacity of absorbing nutrients from aquaculture wastewater [J]. Journal of Tropical Oceanography, 2013, 32(5): 93-98.