Journal of Tropical Oceanography ›› 2021, Vol. 40 ›› Issue (5): 45-52.doi: 10.11978/2020124CSTR: 32234.14.2020124
• Marine Biology • Previous Articles Next Articles
Diversity and anti-bacteria activity of the gorgonian derived fungi from Weizhou Island of Guangxi Province*
LU Chunju1(
), LU Meilin1, LIU Xinming1, LIU Yonghong1,2, GAO Chenghai1, XU Xinya1()
- 1. Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China
2. Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
-
Received:2020-10-30Revised:2021-01-27Online:2021-09-10Published:2021-01-25 -
Contact:XU Xinya E-mail:2250562004@qq.com;xyxu@gxtcmu.edu.cn -
Supported by:National Natural Science Foundation of China(42066006);National Natural Science Foundation of China(41606186);Guangxi Natural Science Foundation(AD19245116);Development Program of High level Talent Team under Qihuang Project of Guangxi University of Chinese Medicine(2018006);Special Fund for Bagui Scholars of Guangxi(05019055);Initial Scientific Research Foundation of Introduced Doctors in 2019 of GXUCM(2018BS039);Scientific Research Foundation of Institute of Marine Drugs, GUCM(2018ZD005);Guangxi First-class Discipline: Chinese Materia Medica(Scientific Research of Guangxi Education Department)([2018] No. 12)
CLC Number:
- P735.51
Cite this article
LU Chunju, LU Meilin, LIU Xinming, LIU Yonghong, GAO Chenghai, XU Xinya. Diversity and anti-bacteria activity of the gorgonian derived fungi from Weizhou Island of Guangxi Province*[J].Journal of Tropical Oceanography, 2021, 40(5): 45-52.
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Tab. 1
Numbers of isolates from the 10 gorgonians"
| 柳珊瑚样本编号 | 共附生真菌分离数 | 总计 | |||
|---|---|---|---|---|---|
| 虎红培养基 | 查氏培养基 | 马铃薯葡萄糖琼脂培养基 | 沙氏培养基 | ||
| 1 | 10 | 7 | 5 | 14 | 36 |
| 2 | 7 | 10 | 6 | 6 | 29 |
| 3 | 3 | 4 | 3 | 0 | 10 |
| 4 | 12 | 5 | 7 | 9 | 33 |
| 5 | 0 | 0 | 1 | 0 | 1 |
| 6 | 1 | 1 | 4 | 1 | 7 |
| 7 | 3 | 1 | 3 | 0 | 7 |
| 8 | 12 | 10 | 7 | 1 | 30 |
| 9 | 8 | 8 | 5 | 7 | 28 |
| 10 | 4 | 3 | 3 | 0 | 10 |
| 总计 | 60 | 49 | 44 | 38 | 191 |
Tab. 1
Tab. 1
Identification of fungal strains isolated from gorgonians based on DNA analysis of the ITS region"
| 菌株编号 | GenBan登录号 | 序列长度/bp | 最相近菌株(BLAST) | 相似度/% | GenBan登录号 |
|---|---|---|---|---|---|
| GXIMD00501 | MT672589 | 533 | Parengyodontium album | 100 | MN944461.1 |
| GXIMD00502 | MT672603 | 535 | Aspergillus niger | 100 | KX622774.1 |
| GXIMD00503 | MT672604 | 516 | Nigrospora oryzae | 100 | KT824761.1 |
| GXIMD00504 | MT672605 | 508 | Cladosporium sp. | 99.80 | MH325933 |
| GXIMD00505 | MT707929 | 515 | Cladosporium sp. | 99.61 | KT989416.1 |
| GXIMD00506 | MT672606 | 511 | Cladosporium halotolerans | 100 | KU216734.1 |
| GXIMD00507 | MT672611 | 512 | Penicillium citrinum | 100 | MK736919.1 |
| GXIMD00508 | MT672612 | 515 | Alternaria alternata | 100 | MK518403.1 |
| GXIMD00509 | MT672613 | 541 | Aspergillus micronesiensis | 99.82 | MT312759.1 |
| GXIMD00510 | MT672614 | 557 | Aspergillus flavipes | 99.64 | MN956655.1 |
| GXIMD00511 | MT672615 | 530 | Aspergillus wentii | 100 | MH855236.1 |
| GXIMD00512 | MT672616 | 540 | Aspergillus sp. | 99.81 | EF672305.1 |
| GXIMD00513 | MT672617 | 518 | Aspergillus sp. | 100 | MH665645.1 |
| GXIMD00514 | MT672618 | 518 | Aspergillus sp. | 100 | MK431431.1 |
| GXIMD00515 | MT672619 | 575 | Aspergillus penicillioides | 99.83 | GU017494.1 |
| GXIMD00516 | MT672620 | 502 | Aspergillus sydowii | 100 | MH793853.1 |
| GXIMD00518 | MT672622 | 509 | Penicillium cinnamopurpureum | 100 | KU847973.1 |
| GXIMD00519 | MT672623 | 513 | Aspergillus carneus | 99.81 | MH777426.1 |
| GXIMD00520 | MT672624 | 508 | Aspergillus tabacinus | 100 | MT635280.1 |
| GXIMD00521 | MT672625 | 519 | Aspergillus unguis | 100 | MH654997.1 |
| GXIMD00522 | MT672626 | 519 | Aspergillus restrictus | 100 | KT959332.1 |
| GXIMD00517 | MT672621 | 540 | Aspergillus versicolor | 99.81 | LC105686.1 |
| GXIMD00523 | MT742624 | 519 | Aspergillus versicolor | 100 | KX674616.1 |
| GXIMD00524 | MT742625 | 514 | Aspergillus versicolor | 99.81 | MF476045.1 |
| GXIMD00525 | MT742626 | 532 | Aspergillus versicolor | 100 | KX776009.1 |
| GXIMD00526 | MT742624 | 517 | Aspergillus versicolor | 99.81 | MN310533.1 |
Tab. 1
Fig. 1
Neighbor-Joining tree based on fungal ITS-rDNA sequences of 26 fungal isolates"
Fig. 1
Tab. 3
Antibacterial activities of ethyl acetate (EtOAc) extracts from fermentation broth of the gorgonian-derived fungal strains"
| 菌株 | 抑制率/% | ||
|---|---|---|---|
| 表皮葡萄球菌 | 无乳链球菌 | 海豚链球菌 | |
| Penicillium citrinum GXIMD00507 | 77.44 | 78.35 | 86.36 |
| Alternaria alternata GXIMD00508 | 63.64 | - | - |
| Aspergillus micronesiensis GXIMD00509 | 70.99 | 58.77 | 68.24 |
| Aspergillus flavipes GXIMD005010 | 57.60 | - | - |
| Aspergillus wentii GXIMD00511 | 76.90 | 69.33 | 81.22 |
| Aspergillus sydowii GXIMD00516 | 62.96 | - | 67.22 |
| Aspergillus versicolor GXIMD00517 | 58.42 | 62.05 | 80.02 |
| Penicillium cinnamopurpureum GXIMD00518 | 86.48 | 79.81 | 85.52 |
| Aspergillus carneus GXIMD00519 | 59.25 | - | 76.38 |
| Aspergillus tabacinus GXIMD00520 | 58.56 | - | 65.50 |
| Aspergillus unguis GXIMD00521 | 88.17 | 80.57 | 86.19 |
| 青霉素 | 91.44 | 73.93 | 84.93 |
Tab. 3
Tab. 4
Anti-biofilm activities of EtOAc extracts from fermentation broth of the gorgonian-derived fungal strains"
| 菌株 | 耐甲氧西林金黄色葡萄球菌 | 表皮葡萄球菌 | |||
|---|---|---|---|---|---|
| MIC50/(μg•mL-1) | EC50/(μg•mL-1) | MIC50/(μg•mL-1) | EC50/(μg•mL-1) | ||
| Penicillium citrinum GXIMD00507 | >200 | - | >200 | 150.6 | |
| Aspergillus micronesiensis GXIMD00509 | >200 | - | >200 | 154.8 | |
| Aspergillus wentii GXIMD00511 | >200 | - | >200 | 40.3 | |
| Penicillium cinnamopurpureum GXIMD00518 | >200 | 105.4 | >200 | 102.9 | |
| Aspergillus carneus GXIMD00519 | >200 | 117.4 | >200 | 105.0 | |
| Aspergillus tabacinus GXIMD00520 | >200 | - | >200 | 66.3 | |
| Aspergillus versicolor GXIMD00525 | >200 | - | >200 | 155.0 | |
| 青霉素 | 6.67 | - | - | - | |
Tab. 4
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