不同分子量海蚌类肝素理化性质分析及抗血栓活性评价

doi:10.11978/2022015

Abstract

Abstract:

To investigate the physicochemical properties in vivo antithrombotic activity and their effects on the bleeding time of different molecular weights of clam heparinoid, native clam heparinoid G2 (60.25 kDa), and its two different degradation products, DG1 (24.48 kDa) and DG2 (6.75 kDa), were taken as the research objects in this study. The content of glucuronic acid and the sulfate group was respectively determined by the modified sulfuric acid-carbazole method and BaCl₂-gel turbidimetric method, the particle size and zeta potential were determined by nanoparticle sizer, and the thermal stability was analyzed by the simultaneous thermal analyzer. Then the antithrombotic activity and the effect on bleeding time were investigated by the mice black tail model and mice broken tail model, respectively. The results showed that as the molecular weight of clam heparinoid decreased, the sulfate content increased, the glucuronic acid content decreased, the initial degradation temperature increased and then decreased, the particle size decreased, and the Zeta potential increased. The moderate degradation product of G2, DG1, has a higher ability to inhibit tail thrombosis with a lower risk of hemorrhage compared to the natural clam heparinoid G2.

Key words: clam heparinoid, physicochemical analysis, antithrombotic activity, bleeding time

CHEN Guanlan, CHEN Jianping, LI Rui, JIA Xuejing, LIU Xiaofei, SONG Bingbing, ZHONG Saiyi. Physicochemical analysis and evaluation of the antithrombotic activity of different molecular weights of heparin from clam Coelomactra antiquata[J].Journal of Tropical Oceanography, 2023, 42(1): 152-160.

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References 34

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样品名称	硫酸基含量/%	糖醛酸含量/%
G2	18.07±1.28	27.17±1.57
DG1	24.74±0.64	22.38±0.93
DG2	27.33±2.22	15.32±3.23

样品名称	粒径/nm	PDI	Zeta电位/mV
G2	262.3±5.3	0.467±0.0218	-31.8±1.6
DG1	183.9±6.2	0.429±0.0339	-25.1±1.0
DG2	152.8±10.6	0.444±0.0169	-17.9±0.6

组别	W₀/g	W₇/g
B	25.3±3.4	32.8±2.5*
M	23.8±3.0	32.9±2.6*
Y	24.6±2.5	34.6±3.5*
G2L	24.6±3.5	33.4±1.9*
G2M	25.6±3.1	34.0±2.6*
G2H	24.5±2.9	33.6±1.9*
DG1L	24.0±3.0	34.8±2.2*
DG1M	23.6±2.2	35.6±2.7*
DG1H	24.4±3.3	33.0±2.4*
DG2L	24.4±3.5	33.6±3.0*
DG2M	23.9±3.8	32.1±1.8*
DG2H	23.9±3.2	31.6±3.4*

组别	黑尾比率/%
组别	24h	48h
B	0±0	0±0
M	52.03±5.46##	57.73±8.89##
Y	28.92±0.56**	33.01±1.61**
G2L	48.67±2.78	45.39±4.43
G2M	35.01±1.41**	33.32±2.09**
G2H	31.30±3.23**	30.83±4.34**
DG1L	46.91±5.27	45.05±3.88
DG1M	36.96±3.19*	34.31±1.68**
DG1H	19.94±5.26**	18.70±2.82**
DG2L	47.82±5.67	49.16±4.71
DG2M	47.30±4.70	47.25±6.61
DG2H	43.95±6.54	45.00±5.42

Physicochemical analysis and evaluation of the antithrombotic activity of different molecular weights of heparin from clam Coelomactra antiquata

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