红树无瓣海桑果实中糖苷鉴定及其延缓线虫衰老活性研究

doi:10.11978/2024150

Abstract

Abstract:

In order to obtain compounds with anti-aging activity in the fruit of Sonneratia apetala, the chemical constituents of n-butanol alcohol extract were separated and their bioactivities were studied. Compounds were isolated and purified by reverse silica column, gel chromatography and high pressure liquid chromatography. The structure of compounds was identified by modern spectral analysis and literature comparison. The anti-aging activity of compounds in Caenorhabditis elegans was investigated using the lifespan, heat stress, pharyngeal pumping capacity, body bending ability and lipofuscin as the indicators. Molecular docking was used in predicting the interaction mode of compounds with protein DAF-16. Five compounds were isolated from the n-butanol alcohol extract for the first time, identified as 1, 2, 3, 6-tetra-O-galloyl -β-D-glucopyranose (1), hovetrichoside C (2), (6R, 9S)-3-oxo-α-ionol-β-D-glucoside (3), kaempferol-3-O-β-D glucoside (4), and 3-methylbutyl β-D-glucopyranoside (5). Compounds 2, 4 and 5 showed an anti-aging activity at 100 μmol·L^-1 concentration significantly, extended the lifespan of C. elegans in normal culture by 15.9%, 15.0% and 22.1% respectively, and significantly prolonged the lifespan under heat stress by 36.3%, 50.6% and 46.3% respectively. They improved the frequency of pharyngeal pumping and the ability of body bending, and reduced the lipofuscin accumulated. Molecular docking showed that compounds 2, 4 and 5 could stably bind to the amino acids of protein DAF-16. The binding energy was -6.6, -6.0 and -4.6 kcal·moL^-1, respectively. This study enriched the material basis of anti-aging activity and provided a basis for the development and utilization of the Sonneratia apetala fruit.

Key words: Sonneratia apetala, glycoside compounds, structural identification, Caenorhabditis elegans, anti-aging

CLC Number:

R284

LIU Jianyun, XIA Jialang, BAI Meng, LI Yueyao, WEI Jinhua, LIU Kai, GAO Chenghai, YI Xiangxi. The identification of glycosides from the fruit of mangrove Sonneratia apetala and investigation of their anti-aging activity in Caenorhabditis elegans[J].Journal of Tropical Oceanography, 2025, 44(3): 148-156.

Figures/Tables 8

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药物分组	药物浓度/(μmol·L^-1)	寿命/d			延长比例/%
药物分组	药物浓度/(μmol·L^-1)	平均数	最大寿命	P	延长比例/%
对照组	—	9.897±0.546	19	—	—
阳性药	100	12.246±0.632**	23	0.005	23.9
1	100	9.417±0.604	21	0.790	—
2	100	11.474±0.773*	25	0.016	15.9
3	100	10.689±0.668	22	0.154	8.0
4	100	11.383±0.676*	23	0.041	15.0
5	100	12.085±0.909**	26	0.004	22.1

药物分组	药物浓度/(μmol·L^-1)	寿命/h			延长比例/%
药物分组	药物浓度/(μmol·L^-1)	平均数	最大寿命	P	延长比例/%
对照组	—	12.660±0.825	21	—	—
阳性药	100	21.140±0.732***	29	<0.001	70.0
2	100	17.260±0.738***	27	<0.001	36.3
4	100	19.071±0.799***	28	<0.001	50.6
5	100	18.520±0.974***	30	<0.001	46.3

The identification of glycosides from the fruit of mangrove Sonneratia apetala and investigation of their anti-aging activity in Caenorhabditis elegans

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