中国沿海尖刺拟菱形藻的种下分类学研究*

doi:10.11978/2017017

Abstract

Abstract:

To declare intraspecies diversity of Pseudo-nitzschia pungens Hasle, a total of 32 monoclonal strains of P. pungens from Chinese coastal waters were isolated and established. These strains were identified carefully, based on the morphology under both light microscopy and transmission electron microscopy, and on the molecular analysis inferred from the internal transcribed spacer region sequences. Two varieties were identified: P. pungens var. pungens (Grunow) Hasle and P. pungens var. averiensis Lundholm, Churro, Carreira, and Calado. Their morphologies were described and compared with allied taxa. Meanwhile, domoic acid (DA) was detected using a liquid chromatography tandem mass spectrometry (LC-MS/MS) injection, but no detectable DA was found. The results may provide detailed information for further studies of toxin production of Chinese Pseudo-nitzschia strains.

Key words: Pseudo-nitzschia pungens, taxonomy, morphology, internal transcribed spacer, domoic acid

CLC Number:

P735.531

Huanchang DONG, Chunxiu HUANG, Guoshuang XU, Yang LI. Studies on intraspecies diversity of Pseudo-nitzschia pungens from Chinese coastal waters^*[J].Journal of Tropical Oceanography, 2018, 37(1): 12-19.

Figures/Tables 4

Tab. 1

Fig. 1

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References 44

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株系	分离地点	采样时间	种名
MC215	珠海	2013年5月	P. pungens var. pungens
MC218	葫芦岛	2013年8月	P. pungens var. pungens
MC241	惠州	2013年10月	P. pungens var. averiensis
MC255	厦门	2013年11月	P. pungens var. averiensis
MC263	青岛	2013年11月	P. pungens var. averiensis
MC289	青岛	2014年2月	P. pungens var. pungens
MC290	青岛	2014年2月	P. pungens var. pungens
MC295	惠州	2014年4月	P. pungens var. pungens
MC404	惠州	2014年7月	P. pungens var. averiensis
MC830	温州	2015年5月	P. pungens var. pungens
MC831	温州	2015年5月	P. pungens var. pungens
MC841	香港	2015年7月	P. pungens var. averiensis
MC864	宁波	2015年8月	P. pungens var. averiensis
MC867	宁波	2015年8月	P. pungens var. averiensis
MC881	湛江	2015年8月	P. pungens var. averiensis
MC888	湛江	2015年8月	P. pungens var. averiensis
MC899	青岛	2015年9月	P. pungens var. averiensis
MC915	深圳	2015年9月	P. pungens var. averiensis
MC988	台湾海峡	2016年5月	P. pungens var. pungens
MC3052	珠海	2016年5月	P. pungens var. pungens
MC3053	珠海	2016年5月	P. pungens var. averiensis
MC3054	珠海	2016年5月	P. pungens var. pungens
MC3057	珠海	2016年5月	P. pungens var. pungens
MC3058	珠海	2016年5月	P. pungens var. pungens
MC3059	珠海	2016年5月	P. pungens var. pungens
MC3060	珠海	2016年5月	P. pungens var. averiensis
MC3061	珠海	2016年5月	P. pungens var. pungens
MC3062	珠海	2016年5月	P. pungens var. pungens
MC3065	珠海	2016年5月	P. pungens var. pungens
MC3067	珠海	2016年5月	P. pungens var. pungens
MC3119	黄海 N:122°50′, E:32°00′	2016年7月	P. pungens var. pungens
MC3195	台湾海峡	2016年8月	P. pungens var. averiensis

Studies on intraspecies diversity of Pseudo-nitzschia pungens from Chinese coastal waters^*

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Studies on intraspecies diversity of Pseudo-nitzschia pungens from Chinese coastal waters*

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Studies on intraspecies diversity of Pseudo-nitzschia pungens from Chinese coastal waters^*