重金属胁迫下四种红树植物幼苗生理响应特征

doi:10.11978/2021118

热带海洋学报 ›› 2022, Vol. 41 ›› Issue (6): 28-34.doi: 10.11978/2021118CSTR: 32234.14.2021118

重金属胁迫下四种红树植物幼苗生理响应特征

谢勇¹^,²^,³^,⁴(), 王友绍¹^,²^,³^,⁵()

1.热带海洋环境国家重点实验室(中国科学院南海海洋研究所), 广东广州 510301
2.中国科学院大亚湾海洋生物综合实验站, 广东深圳 518121
3.中国科学院南海生态与环境工程创新院, 广东广州 510301
4.中国科学院大学, 北京 100049
5.南方海洋科学与工程广东实验室(广州), 广东广州 511458

收稿日期:2021-09-02 修回日期:2021-09-27 出版日期:2022-11-10 发布日期:2022-11-29
通讯作者: 王友绍
作者简介:谢勇(1996—)，男，湖北省仙桃市人，硕士研究生，从事红树林生态学研究。email: xieyong199605@163.com
基金资助:
国家重点研发计划项目(国家科技基础资源调查专项)(2017FY100700);国家自然科学基金项目(U1901211);国家自然科学基金项目(41876126);国际伙伴计划(133244KYSB20180012);中国科学院A类战略性先导科技专项(XDA23050200);南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0305)

Physiological response characteristics of four mangrove plants seedlings to heavy metal stress

XIE Yong¹^,²^,³^,⁴(), WANG Youshao¹^,²^,³^,⁵()

1. State Key Laboratory of Tropical Oceanography (South China Sea Institute of Oceanology, Chinese Academy of Sciences), Guangzhou 510301, China
2. Daya Bay Marine Biology Research Station, Chinese Academy of Sciences, Shenzhen 518121, China
3. Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou 510301, China
4. University of Chinese Academy of Sciences, Beijing 100049, China
5. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China

Received:2021-09-02 Revised:2021-09-27 Online:2022-11-10 Published:2022-11-29
Contact: WANG Youshao
Supported by:
National Key Research and Development Program of China(2017FY100700);National Natural Science Foundation of China(U1901211);National Natural Science Foundation of China(41876126);International Partnership Program of Chinese Academy of Sciences(133244KYSB20180012);Strategic Priority Research Program of the Chinese Academy of Sciences(XDA23050200);Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)(GML2019ZD0305)

摘要/Abstract

摘要：

研究了四种红树植物红海榄、秋茄、木榄、角果木幼苗在不同浓度重金属水溶液胁迫28天后的生理生化指标变化特征，包括可溶性蛋白含量、超氧化物歧化酶（superoxide dismutase, SOD）、过氧化物酶（peroxidase, POD）活性和氧化损伤标志物丙二醛（malondialdehyde, MDA）含量。研究结果表明四种植物叶片内的SOD、POD活性均呈现出先升高后下降趋势，10倍重金属水溶液中的红海榄，5倍重金属水溶液中的秋茄和木榄，以及1倍重金属水溶液中的角果木具有较好的抗氧化酶活性，能有效清除活性氧，减小氧化损伤。在同等浓度重金属水溶液处理下，MDA含量结果显示红海榄受损程度最轻，秋茄、木榄受损程度居中，角果木受损程度最重。红海榄抗重金属胁迫能力强于秋茄、木榄，角果木抗重金属能力最弱。上述四种红树植物对重金属的抗性结果说明红树科植物具有较强的抗污染能力，这对于我国红树林生态修复与保护具有重要的指导意义。

关键词: 重金属胁迫, 红树植物, 生理响应, 特征

Abstract:

The change characteristics of physio-biochemical indexes of four mangrove plants Rhizophora stylosa, Kandelia obovata, Bruguiera gymnorhiza and Ceriops tagal seedlings after 28 days of heavy metal aqueous solution stress at different concentrations were studied, including soluble protein content, superoxide dismutase (SOD), and peroxidase (POD) activity and oxidative damage marker malondialdehyde (MDA) content. The study results showed that the SOD and POD activities in the leaves of the four plants all increased first and then decreased. When the Rhizophora stylosa were treated with 10 times the heavy metal aqueous solution, the Kandelia obovata and Bruguiera gymnorhiza were treated with 5 times the heavy metal aqueous solution, the Ceriops tagal were treated with 1 time the heavy metal aqueous solution, it has good antioxidant enzyme activity during heavy metal aqueous solution treatment, which can effectively remove active oxygen and reduce oxidative damage. Under the same concentration of heavy metal aqueous solution treatment, the results of MDA content showed that the damage of the Rhizophora stylosa was the least, the Kandelia obovata and the Bruguiera gymnorhiza were equally damaged, and the Ceriops tagal were the heaviest. The ability of Rhizophora stylosa to resist heavy metal stress is stronger than Kandelia obovate and Bruguiera gymnorhiza, while the ability of Ceriops tagal to resist heavy metal is the weakest. These results show that mangrove plants have strong ability for anti-pollution, which has important guiding significance for mangrove ecological restoration and protection in China.

Key words: heavy metal stress, mangrove plants, physiological response, characteristics

中图分类号:

Q945.78

谢勇, 王友绍. 重金属胁迫下四种红树植物幼苗生理响应特征[J]. 热带海洋学报, 2022, 41(6): 28-34.

XIE Yong, WANG Youshao. Physiological response characteristics of four mangrove plants seedlings to heavy metal stress[J]. Journal of Tropical Oceanography, 2022, 41(6): 28-34.

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重金属	浓度/(mg·L^-1)
重金属	对照组(CK)	1倍水溶液(T1)	5倍水溶液(T2)	10倍水溶液(T3)	15倍水溶液(T4)
Cu²⁺	0	7.5	37.5	75	112.5
Pb²⁺	0	1.5	7.5	15	22.5
Cd²⁺	0	0.3	1.5	3	4.5
Hg²⁺	0	0.3	1.5	3	4.5

重金属胁迫下四种红树植物幼苗生理响应特征

Physiological response characteristics of four mangrove plants seedlings to heavy metal stress

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	可溶性蛋白	SOD	POD	MDA
可溶性蛋白	1
SOD	-0.088	1
POD	-0.935^**	0.292	1
MDA	-0.834^**	0.087	0.836^**	1

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