盐度对中国鲎幼鲎生长、蜕壳、Na+-K+-ATP酶活性、免疫指标和抗氧化能力的影响

doi:10.11978/2021106

Abstract

Abstract:

Artificial proliferation and release is the most effective and rapid method to restore Limulus resources. Understanding and grasping the influence of environmental factors on the growth of juvenile Chinese horseshoe crab (Tachypleus tridentatus), and then choosing a suitable time and sea area for release are the key steps to ensure the success of manual release. Under laboratory conditions, we studied the effects of different salinity (5‰, 10‰, 15‰, 20‰, 25‰, 30‰, 35‰, and 40‰) on the survival rate, molting rate, molting weight gain rate, Na⁺-K⁺-ATPase activity, immune enzyme activity, and antioxidant enzyme activity of juvenile Chinese horseshoe crab. The changes of growth, molting, osmotic adjustment, immunity, and antioxidation of the juvenile horseshoe crab at different salinity levels were recorded. The culture experiment lasted for 56 days. The results showed that different salinity levels significantly affected the survival rate, molting rate, average weight of the 2nd instar horseshoe crabs, and molting weight gain rate of the juvenile horseshoe crab (P< 0.05), which increased at first and then decreased with increasing salinity. Regression analysis of molting rate, molting weight gain rate and salinity showed that the optimum salinity for molting and growth of young horseshoe crab were 24.10‰ and 24.94‰, respectively. The Na⁺-K⁺-ATPase activities of the 1st and 2nd instar horseshoe crabs increased at first and then decreased significantly with the increase of salinity (P< 0.05). The acid phosphatase (ACP) activities of the 1st instar larvae in the 35‰ and 40‰ test groups were significantly higher than those in the other test groups, while the alkaline phosphatase (AKP) activities of the 5‰ test group were significantly lower than those in the 25‰ test group (P< 0.05). The activities of ACP, AKP and lysozyme (LZM) of the 2nd instar larvae did not change significantly under different salinity conditions (P> 0.05). The catalase (CAT) activities of the 1st instar larvae in 35‰ and 40‰ test groups were significantly higher than those of low-salinity test groups (P< 0.05). And the glutathione peroxidase (GSH-Px) activities of the 2nd WHAT tended to increase with the increase of salinity, and those in 10‰ test group were significantly lower than those in 35‰ and 40‰ test groups (P< 0.05). We show that salinity had a significant effect on growth, molting, Na⁺-K⁺-ATPase activity, immune index, and antioxidation of the juvenile horseshoe crab. The optimum salinity for molting was between 24‰ and 25‰. Too high or too low salinity will cause the growth rate, survival rate of juvenile silver carp, the osmotic adjustment ability, immunity, and antioxidation of the juvenile horseshoe crab to decrease significantly.

Key words: salinity, juvenile Tachypleus tridentatus, molting, Na⁺-K⁺-ATPase, immune enzyme, antioxidation

CLC Number:

S917.4

DONG Lanfang, XU Mingzhu, LIU Haijuan, ZENG Mengqing, CHEN Ruifang, LI Shicai. Effects of salinity on growth, molting, Na⁺-K⁺-ATP enzyme activities, immune index, and antioxidantion of juvenile Chinese horseshoe crab, Tachypleus tridentatus[J].Journal of Tropical Oceanography, 2022, 41(3): 156-163.

Figures/Tables 5

Tab. 1

Fig. 1

Fig. 2

Tab. 2

Tab. 3

References 33

[1]	鲍虞园, 叶国玲, 颉晓勇, 2020. 中国鲎人工繁育及1龄稚鲎形态性状对体质量的影响[J]. 渔业科学进展, 41(4): 77-84.
	BAO YUYUAN, YE GUOLING, XIE XIAOYONG, 2020. Effects of morphological characters on body weight of artificially bred the first instars of Horseshoe Crabs (Tachypleus tridentatus)[J]. Progress in Fishery Sciences, 41(4): 77-84. (in Chinese with English abstract)
[2]	蔡星媛, 张秀梅, 田璐, 等, 2015. 盐度胁迫对魁蚶稚贝血淋巴渗透压及鳃Na⁺/K⁺-ATP酶活力的影响[J]. 南方水产科学, 11(2): 12-19.
	CAI XINGYUAN, ZHANG XIUMEI, TIAN LU, et al, 2015. Effect of salinity stress on hemolymph osmolality and gill Na⁺/K⁺-ATPase activity of juvenile ark shell (Anadara broughtonii)[J]. South China Fisheries Science, 11(2): 12-19. (in Chinese with English abstract)
[3]	陈垂坤, 郜卫华, 田罗, 2015. 盐度和营养素对甲壳动物营养生理的影响研究进展[J]. 长江大学学报(自然科学版), 12(3): 47-51. (in Chinese)
[4]	程鹏, 周爱娜, 霍淑芳, 等, 2006. 中国鲎人工培育的幼体对不同环境适应性的研究[J]. 厦门大学学报(自然科学版), 45(3): 404-408.
	CHENG PENG, ZHOU AINA, HUO SHUFANG, et al, 2006. Study on adaptability to different environment for juvenile of horseshoe crab, Tachypleus tridentatus with artificial incubation[J]. Journal of Xiamen University (Natural science), 45(3): 404-408. (in Chinese with English abstract)
[5]	程文, 2007. 中国鲎的胚胎发育及温度和盐度对一期幼体代谢的影响[D]. 武汉: 华中农业大学: 26-33.
	CHENG WEN, 2007. Embryonic development and the effects of temperature and salinity on the metabolism of the first instars of Tachypleus tridentatus[D]. Wuhan: Huazhong Agricultural University: 26-33. (in Chinese with English abstract)
[6]	高凤英, 廖永岩, 叶富良, 2003. 中国鲎幼体阶段(黄皮鲎)饵料的初步研究[J]. 海洋通报, 22(4): 92-96.
	GAO FENGYING, LIAO YONGYAN, YE FULIANG, 2003. Feed study of the Tachypleus tridentatus juvenile[J]. Marine Science Bulletin, 22(4): 92-96. (in Chinese with English abstract)
[7]	洪水根, 2011. 中国鲎生物学研究[M]. 厦门: 厦门大学出版社: 58-62.
	HONG SHUIGEN, 2011. Biology of horseshoe crabs, Tachypleus tridentatus[M]. Xiamen: Xiamen University Press: 58-62. (in Chinese)
[8]	蒋湘, 谢妙, 彭树锋, 等, 2017. 盐度对日本囊对虾生长与存活率的影响[J]. 江苏农业科学, 45(16): 152-155.
	JIANG XIANG, XIE MIAO, PENG SHUFENG, et al, 2017. Effects of salinity on growth and survival rate of Marsupenaeus japonicas[J]. Jiangsu Agricultural Sciences, 45(16): 152-155. (in Chinese with English abstract)
[9]	李锋, 廖永岩, 董学兴, 1999. 盐度对中国鲎(Tachypleus tridentatus)胚胎发育的影响[J]. 湛江海洋大学学报, 19(3): 4-8.
	LI FENG, LIAO YONGYAN, DONG XUEXING, 1999. The influence of salinity upon enbryogeny of Tachypleus tridentatus[J]. Journal of Zhanjiang Ocean University, 19(3): 4-8. (in Chinese with English abstract)
[10]	李娜, 王仁杰, 赵玉超, 等, 2017. 高盐胁迫对凡纳滨对虾生长指标、血浆渗透压及Na⁺-K⁺-ATP酶活力的影响[J]. 浙江海洋学院学报(自然科学版), 36(3): 196-201.
	LI NA, WANG RENJIE, ZHAO YUCHAO, et al, 2017. Effects of high salinity on growth index, plasma osmotic pressure and Na⁺-K⁺-ATPase activities of Litopenaeus vannamei[J]. Journal of Zhejiang Ocean University (Natural Science), 36(3): 196-201. (in Chinese with English abstract)
[11]	李琼珍, 2010. 中国鲎保育工作研究进展[J]. 生物学杂志, 27(4): 71-74.
	LI QIONGZHEN, 2010. Advance on conservation of Chinese horseshoe crab (Tachypleus tridentatus)[J]. Journal of Biology, 27(4): 71-74. (in Chinese with English abstract)
[12]	李琼珍, 胡梦红, 2011. 广西北海中国鲎资源现状以及保育工作进展[J]. 海洋环境科学, 30(1): 131-134.
	LI QIONGZHEN, HU MENGHONG, 2011. Status of Chinese horseshoe crab population and conservation advance in Beihai, Guangxi[J]. Marine Environmental Science, 30(1): 131-134. (in Chinese with English abstract)
[13]	李志辉, 王玉, 李光光, 等, 2019. 温度和盐度对脊尾白虾生长和非特异性免疫的影响[J]. 海洋湖沼通报, (6): 78-82.
	LI ZHIHUI, WANG YU, LI GUANGGUANG, et al, 2019. Effects of temperature and salinity on the growth and non-specific immune indices of Exopalaemon carinicauda[J]. Transactions of Oceanology and Limnology, (6): 78-82. (in Chinese with English abstract)
[14]	梁君荣, 王军, 苏永全, 等, 2001. 四种重金属对中国鲎(Tachypleus tridentatus)胚胎发育的影响[J]. 生态学报, 21(6): 1009-1012.
	LIANG JUNRONG, WANG JUN, SU YONGQUAN, et al, 2001. Effects of metals on embryodevelopment of Tachypleus tridentatus[J]. Acta Ecologica Sinica, 21(6): 1009-1012. (in Chinese with English abstract)
[15]	柳旭东, 王际英, 张利民, 等, 2009. 影响水产动物Na⁺-K⁺-ATP酶活力的水环境因素[J]. 水产科学, 28(3): 171-175.
	LIU XUDONG, WANG JIYING, ZHANG LIMIN, et al, 2009. Water environmental factors affecting Na⁺-K⁺-ATPase activity in aquatic animals[J]. Fisheries Science, 28(3): 171-175. (in Chinese with English abstract)
[16]	龙晓文, 吴旭干, 刘智俊, 等, 2014. 盐度对脊尾白虾存活、生长和蜕壳的影响[J]. 广东农业科学, 41(23): 111-115, 130.
	LONG XIAOWEN, WU XUGAN, LIU ZHIJUN, et al, 2014. Effects of salinity on survival, growth and molt of Exopalaemon carinicauda[J]. Guangdong Agricultural Sciences, 41(23): 111-115, 130. (in Chinese with English abstract)
[17]	路允良, 王芳, 赵卓英, 等, 2012. 盐度对三疣梭子蟹生长、蜕壳及能量利用的影响[J]. 中国水产科学, 19(2): 237-245. doi: 10.3724/SP.J.1118.2012.00237
	LU YUNLIANG, WANG FANG, ZHAO ZHUOYING, et al, 2012. Effects of salinity on growth, molt and energy utilization of juvenile swimming crab Portunus trituberculatus[J]. Journal of Fishery Sciences of China, 19(2): 237-245. (in Chinese with English abstract) doi: 10.3724/SP.J.1118.2012.00237
[18]	亓磊, 顾孝连, 蒋科技, 等, 2013. 盐度对拟穴青蟹幼蟹存活、生长和Na⁺/K⁺-ATP酶活性的影响[J]. 海洋科学, 37(2): 56-60.
	QI LEI, GU XIAOLIAN, JIANG KEJI, et al, 2013. Effect of salinity on the survival, growth and Na⁺/K⁺-ATPase activity of early juvenile mud crabs, Scylla paramamosain[J]. Marine Sciences, 37(2): 56-60. (in Chinese with English abstract)
[19]	申玉春, 陈作洲, 刘丽, 等, 2012. 盐度和营养对凡纳滨对虾蜕壳和生长的影响[J]. 水产学报, 36(2): 290-299. doi: 10.3724/SP.J.1231.2012.27736
	SHEN YUCHUN, CHEN ZUOZHOU, LIU LI, et al, 2012. The effects of salinity and nutrition on molt and growth of Litopenaeus vannamei[J]. Journal of fisheries of China, 36(2): 290-299. (in Chinese with English abstract) doi: 10.3724/SP.J.1231.2012.27736
[20]	王德祥, 苏永全, 王军, 等, 2001. 几种因子对中国鲎胚胎和幼体发育的影响[J]. 中国水产科学, 8(3): 10-14.
	WANG DEXIANG, SU YONGQUAN, WANG JUN, et al, 2001. Influence of environmental factors on development of embryo and larvae in Tachypleus tridentatues[J]. Journal of Fishery Sciences of China, 8(3): 10-14. (in Chinese with English abstract)
[21]	王军, 王德祥, 苏永全, 等, 2001. 中国鲎的胚胎发育[J]. 动物学杂志, 36(4): 9-14.
	WANG JUN, WANG DEXIANG, SU YONGQUAN, et al, 2001. Embryonic development of Tachypleus tridentatus[J]. Chinese Journal of Zoology, 36(4): 9-14. (in Chinese with English abstract)
[22]	翁朝红, 谢仰杰, 肖志群, 等, 2012. 福建及中国其他沿岸海域中国鲎资源分布现状调查[J]. 动物学杂志, 47(3): 40-48.
	WEN ZHAOHONG, XIE YANGJIE, XIAO ZHIQUN, et al, 2012. Distribution and resource of Chinese horseshoe crab (Tachypleus tridentatus) in Fujian and other coast water of China[J]. Chinese Journal of Zoology, 47(3): 40-48. (in Chinese with English abstract)
[23]	吴丹华, 郑萍萍, 张玉玉, 等, 2010. 温度胁迫对三疣梭子蟹血清中非特异性免疫因子的影响[J]. 大连水产学院学报, 25(4): 370-375.
	WU DANHUA, ZHENG PINGPING, ZHANG YUYU, et al, 2010. Effect of temperature stress on serum non-specific immune factors in swimming crab Portunus trituberculatus[J]. Journal of Dalian Ocean University, 25(4): 370-375. (in Chinese with English abstract)
[24]	颜明艳, 李琼珍, 宋洁, 等, 2019. 基于MAXENT模型评估北部湾潮间带中国鲎和圆尾鲎稚鲎的潜在地理分布及种群保育对策[J]. 生态学报, 39(9): 3100-3109.
	YAN MINGYAN, LI QIONGZHEN, SONG JIE, et al, 2019. Prediction of potential distribution areas of Chinese horseshoe crab and mangrove horseshoe crab in the Beibu Gulf of Guangxi based on MAXENT model and their population conservation strategies[J]. Acta Ecologica Sinica, 39(9): 3100-3109. (in Chinese with English abstract)
[25]	杨其彬, 叶乐, 温为庚, 等, 2008. 盐度对斑节对虾蜕壳、存活、生长和饲料转化率的影响[J]. 南方水产, 4(1): 16-21.
	YANG QIBIN, YE LE, WEN WEIGENG, et al, 2008. Effect of salinity on molting, survival, growth and feed conversion rate of juvenile Penaeus monodon[J]. South China Fisheries Science, 4(1): 16-21. (in Chinese with English abstract)
[26]	杨宇晴, 余德光, 谢骏, 等, 2010. 急性盐度胁迫对斜带石斑鱼Na⁺/K⁺-ATP酶及血清应激指标的影响[J]. 热带海洋学报, 29(4): 160-164.
	YANG YUQING, YU DEGUANG, XIE JUN, et al, 2010. Effects of acute salinity stress on Na⁺/K⁺-ATPase activity and plasma indicators of Epinephelus coioides[J]. Journal of Tropical Oceanography, 29(4): 160-164. (in Chinese with English abstract)
[27]	游出超, 董兰芳, 曾梦清, 等, 2019. 急性低盐度胁迫对可口革囊星虫存活、Na⁺/K⁺-ATP酶及磷酸酶活力的影响[J]. 海洋科学, 43(3): 82-89.
	YOU CHUCHAO, DONG LANFANG, ZENG MENGQING, et al, 2019. Effect of acute low-salinity stress on the survival and Na⁺/K⁺-ATPase and phosphatase activities of Phascolosoma esculenta[J]. Marine Sciences, 43(3): 82-89. (in Chinese with English abstract)
[28]	于忠利, 乔振国, 王建纲, 2010. 盐度和水流对青蟹蜕壳及存活的影响[J]. 海洋科学, 34(10): 68-70.
	YU ZHONGLI, QIAO ZHENGUO, WANG JIANGANG, 2010. Effects of salinity and water current on the molting and survival rate of Scylla serrata[J]. Marine Sciences, 34(10): 68-70. (in Chinese with English abstract)
[29]	赵国庆, 邱盛尧, 黄洪亮, 等, 2018. 盐度骤变和渐变对南极大磷虾生存状况和蜕壳的影响[J]. 海洋渔业, 40(3): 360-367.
	ZHAO GUOQING, QIU SHENGYAO, HUANG HONGLIANG, et al, 2018. Effects of abrupt and gradual changes in salinity on survival status and molting of Euphausia superba[J]. Marine Fisheries, 40(3): 360-367. (in Chinese with English abstract)
[30]	朱俊华, 吴宙, 冯炳斌, 等, 2020. 全球中华鲎资源保护现状及对策建议[J]. 生物多样性, 28(5): 621-629. doi: 10.17520/biods.2019401
	ZHU JUNHUA, WU ZHOU, FENG BINGBIN, et al, 2020. Global conservation of Tachypleus tridentatus: present status and recommendations[J]. Biodiversity Science, 28(5): 621-629. (in Chinese with English abstract) doi: 10.17520/biods.2019401
[31]	MARTÍNEZ-ÁLVAREZ R M, HIDALGO M C, DOMEZAIN A, et al, 2002. Physiological changes of sturgeon Acipenser naccarii caused by increasing environmental salinity[J]. Journal of Experimental Biology, 205(23): 3699-3706. doi: 10.1242/jeb.205.23.3699
[32]	ROMANO N, ZENG CHAOSHU, 2006. The effects of salinity on the survival, growth and haemolymph osmolality of early juvenile blue swimmer crabs, Portunus pelagicus[J]. Aquaculture, 260(1-4): 151-162. doi: 10.1016/j.aquaculture.2006.06.019
[33]	SEKIGUCHI K, 1988. Biology of horseshoe crabs[M]. Tokyo: Science House Co. Ltd.: 23-39.

盐度/‰	成活率/%	蜕壳率/%	一龄幼鲎平均体质量/mg	二龄幼鲎平均体质量/mg	蜕壳增重率/%
5	74.81±5.01^a	42.17±6.89^a	34.57±1.81	49.26±1.59^a	42.58±3.03^a
10	83.70±2.57^ab	64.70±5.65^b	33.16±2.88	53.94±2.35^ab	63.17±2.03^bc
15	88.15±1.28^bc	75.62±8.82^bc	34.53±2.30	55.60±3.87^ab	61.02±2.11^bc
20	96.30±6.41^c	82.96±7.14^c	35.08±3.21	56.62±3.68^ab	61.70±3.71^bc
25	93.33±2.23^bc	92.08±1.20^c	33.33±1.45	57.30±2.29^b	71.92±2.54^c
30	87.41±4.63^bc	91.46±1.97^c	32.44±2.37	55.39±2.05^ab	70.98±3.99^c
35	85.55±4.01^abc	85.89±1.47^c	33.63±1.59	55.20±2.46^ab	64.14±2.46^bc
40	87.41±1.28^bc	46.69±9.47^a	34.81±0.37	53.72±1.54^ab	54.31±1.59^b

盐度/‰	一龄幼鲎			二龄幼鲎
盐度/‰	LZM/(μg·mL^-1)	ACP/(U·gprot^-1)	AKP/(U·gprot^-1)	LZM/(μg·mL^-1)	ACP/(U·gprot^-1)	AKP/(U·gprot^-1)
5	48.86±1.67	9.38±0.45^a	59.14±2.23^a	68.27±9.02	7.65±0.43	37.30±9.66
10	51.32±5.50	9.16±0.11^a	84.39±11.01^b	72.17±13.57	7.39±0.37	32.93±4.68
15	49.98±10.57	9.08±0.32^a	87.02±7.07^b	66.34±11.78	7.28±0.19	31.52±5.06
20	51.56±1.89	9.69±0.38^a	86.72±5.66^b	70.30±7.01	7.33±0.31	34.37±5.75
25	49.75±4.45	9.90±0.94^a	82.83±4.49^b	66.55±4.59	7.29±0.16	35.53±3.98
30	49.79±4.81	9.61±0.32^a	83.67±2.11^b	69.69±4.59	7.24±0.37	35.63±4.18
35	51.69±1.12	12.22±0.96^b	85.56±8.10^b	71.60±4.59	7.25±0.28	35.94±6.86
40	50.60±2.55	12.46±0.96^b	84.85±5.60^b	69.33±4.59	7.44±0.18	38.11±7.69

盐度/‰	一龄幼鲎				二龄幼鲎
盐度/‰	T-AOC /(U·mgprot^-1)	GSH-Px /(U·mgprot^-1)	CAT /(U·mgprot^-1)	SOD /(U·mgprot^-1)	T-AOC /(U·mgprot^-1)	GSH-Px /(U·mgprot^-1)	CAT /(U·mgprot^-1)	SOD /(U·mgprot^-1)
5	2.03±0.32	3.23±1.05	17.13±2.11^a	49.30±1.58	3.55±0.19	8.43±0.64^ab	47.65±8.52	143.64±10.01
10	1.70±0.25	3.60±1.15	17.37±1.68^a	50.54±1.36	3.07±0.58	7.48±0.74^a	50.73±6.06	145.03±15.40
15	1.71±0.19	3.10±0.30	18.12±1.20^a	50.01±1.51	3.08±0.56	9.97±0.80^b	54.21±4.54	140.72±22.37
20	1.65±0.13	3.67±1.22	17.48±1.16^a	51.94±2.79	2.92±0.45	9.60±1.47^ab	49.66±1.91	150.22±10.39
25	1.63±0.18	3.80±0.50	18.20±0.88^a	50.39±2.25	2.93±0.43	9.87±0.83^ab	49.71±2.29	145.14±11.80
30	1.63±0.14	3.57±0.68	18.40±1.61^a	51.04±1.00	2.98±0.78	10.07±0.35^b	49.77±8.17	145.45±5.25
35	1.86±0.15	3.90±0.26	20.97±1.705^a	50.42±2.01	3.05±0.22	9.80±0.76^ab	49.57±3.35	143.84±11.49
40	1.95±0.06	3.48±1.30	27.11±1.78^b	52.00±1.09	3.07±0.31	10.03±0.91^b	49.48±12.07	142.19±10.00

Effects of salinity on growth, molting, Na⁺-K⁺-ATP enzyme activities, immune index, and antioxidantion of juvenile Chinese horseshoe crab, Tachypleus tridentatus

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 33

Related Articles 15

Metrics

Comments

Recommended 0

[1]	LIN Guihuan, YAN Youfang, LIU Ying. Ocean stratification in the Indonesian-Australian basin and its influencing factors [J]. Journal of Tropical Oceanography, 2024, 43(4): 57-67.
[2]	SUN Wenjie, LI Jiamin, WANG Hualong, MI Tiezhu, ZHEN Yu. Transcriptomic analysis of fatty acid metabolism in the Thalassiosira pseudonana under low salinity stress [J]. Journal of Tropical Oceanography, 2023, 42(5): 92-103.
[3]	TANG Jiaoyu, WANG Weiqiang, XU Kang, ZHANG Zhenqiu. Interannual variability of subsurface high salinity water in eastern equatorial Indian Ocean^* [J]. Journal of Tropical Oceanography, 2023, 42(1): 10-21.
[4]	LYU Hongke, GONG Yuanfa, WANG Guihua. A linear Markov model for the forecast of sea surface salinity in the Indian Ocean and its improvement method [J]. Journal of Tropical Oceanography, 2022, 41(6): 151-158.
[5]	LIAO Ying, XIA Xiaomin. Effects of salinity on the growth and transcriptome of euryhaline Synechococcus sp. K1* [J]. Journal of Tropical Oceanography, 2022, 41(2): 159-169.
[6]	QIU Shuang, YE Haijun, ZHANG Yuhong, TANG Shilin. Multi-linear regression of partial pressure of sea-surface carbon dioxide in the South China Sea and its mechanism [J]. Journal of Tropical Oceanography, 2022, 41(1): 106-116.
[7]	HE Zikang, WANG Xidong, CHEN Zhiqiang, FAN Kaigui. Reconstructing salinity profile using temperature profile and sea surface salinity [J]. Journal of Tropical Oceanography, 2021, 40(6): 41-51.
[8]	LU Mengying, SU Maoliang, ZHANG Junbin. Effects of salinity changes on serum and kidney immune status associated with injection of Aeromonas hydrophila in Scatophagus argus [J]. Journal of Tropical Oceanography, 2021, 40(3): 114-123.
[9]	Xiaolan PAN, Huiru LIU, Meng XU, Hanzhi XU, Hua ZHANG, Maoxian HE. Cloning and expression analysis of aquaporin gene AQP4 cDNA from Pinctada fucata martensii [J]. Journal of Tropical Oceanography, 2020, 39(3): 66-75.
[10]	Jianwei CHI,Tangdong QU,Ying ZHANY,Ping SHI,Yan DU. Seasonal variability of the Eastern Tropical Pacific Fresh Pool ^* [J]. Journal of Tropical Oceanography, 2019, 38(5): 1-9.
[11]	Xiangpeng WANG,Yuhong ZHANG,Aimei WANG,Wei ZHAO,Yan DU. Low-frequency variability of subsurface salinity in the South China Sea and its relationship with the Pacific Decadal Oscillation [J]. Journal of Tropical Oceanography, 2019, 38(4): 1-9.
[12]	Zhujun FANG, Hai ZHI, Pengfei LIN, Xiang WEI. Distinguishing two types of El Niño in the Tropical Pacific using key region sea surface salinity index [J]. Journal of Tropical Oceanography, 2019, 38(2): 32-42.
[13]	Yan XU, Xiangquan LIU, Rengang SONG, Xianrong CEN, Shuangxi GUO, Shengqi ZHOU. Numerical simulation of diffusive convection staircase [J]. Journal of Tropical Oceanography, 2019, 38(1): 11-18.
[14]	Wenhao CAO, Jin YAN, Meiping FENG, Shuaishuai HAN, Mingqing LIN. Effects of salinity on larval development of two common barnacles from the southeast coast of China [J]. Journal of Tropical Oceanography, 2018, 37(6): 85-91.
[15]	Yuanyuan FU, Xuhua CHENG, Yuhong ZHANG, Youfang YAN, Yan DU. The freshening trend of surface salinity in the South China Sea in recent two decades and its mechanism [J]. Journal of Tropical Oceanography, 2017, 36(4): 18-24.

Effects of salinity on growth, molting, Na+-K+-ATP enzyme activities, immune index, and antioxidantion of juvenile Chinese horseshoe crab, Tachypleus tridentatus

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 33

Related Articles 15

Metrics

Comments

Recommended 0

Effects of salinity on growth, molting, Na⁺-K⁺-ATP enzyme activities, immune index, and antioxidantion of juvenile Chinese horseshoe crab, Tachypleus tridentatus