南海软珊瑚共附生真菌Aspergillus sp. EGF15-0-3苯甲醛类化合物研究*

doi:10.11978/2020085

Journal of Tropical Oceanography ›› 2021, Vol. 40 ›› Issue (4): 63-69.doi: 10.11978/2020085CSTR: 32234.14.2020085

• Marine Biology • Previous Articles Next Articles

Research on benzaldehydes from the soft coral-associated symbiotic fungus Aspergillus sp. EGF15-0-3

LIU Bingxin^1,²(), WEI Xia¹, XIAO Xiji¹, ZHANG Qi¹, ZHANG Cuixian¹()

1. School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
2. Kangmei Pharmaceutical Co., Ltd, Guangzhou 510627, China

Received:2020-08-01 Revised:2020-10-16 Online:2021-07-10 Published:2020-12-24
Contact: ZHANG Cuixian E-mail:414040589@qq.com;zhangcuixian @gzucm.edu.cn
Supported by:
Special Project for Marine Economic Development (Six Major Marine Industries) of Guangdong Province Department of Natural Resources of Guangdong Province([2020]039);Science and Technology Planning Project of Guangdong Province(2017A020217008);Science and Technology Planning Project of Guangdong Province(2015A020216017);Fire Plan Project of the Guangzhou University of Chinese Medicine(XH20170110);National Natural Science Foundation of China(81741160);Natural Science Foundation of Guangdong Province(2014A030313411)

Abstract

Abstract:

To research the benzaldehydes of the soft coral-associated symbiotic fungus Aspergillus sp. EGF 15-0-3 with the activity of overcoming tumor resistance, the secondary metabolites were isolated by silica gel column chromatography, ODS, Sephadex LH-20, thin layer chromatography, and high performance liquid chromatography (HPLC). The structures of compounds were identified by nuclear magnetic resonance (NMR), mass spectrometry(MS), and literature analysis. Nine benzaldehydes were obtained and elucidated as follows: flavoglaucin (1), tetrahydroauroglaucin (2), isoaspergin (3), isotetrahydroauroglaucin (4), dihydroauroglaucin (5), isodihydroauroglaucin (6), 2-(1',5'-heptadienyl)-3,6-dihydroxy-5- (3''-methyl-2''-butenyl) benzaldehyde (7), auro- glaucin (8), and 2-(2',3-epoxy-1'-heptenyl)-6- hydroxy-5-(3''methyl-2''-butenyl) benzal-dehyde (9). Using the MTT [3-(4, 5)-dimethylthiahiazo (-z-y1)-3, 5-di-phenytetrazoliumromide] method, compound 9 showed strong activity to drug-resistant tumor cell lines H1975/GR. This may be due to the structure of a pyran ring between the 2-position side chain and 3-hydroxyl group.

Key words: Soft coral-associated symbiotic epiphytic fungus, Aspergillus sp. EGF15-0-3, benzaldehydes, overcoming tumor resistance activity

CLC Number:

P735.51

LIU Bingxin, WEI Xia, XIAO Xiji, ZHANG Qi, ZHANG Cuixian. Research on benzaldehydes from the soft coral-associated symbiotic fungus Aspergillus sp. EGF15-0-3[J].Journal of Tropical Oceanography, 2021, 40(4): 63-69.

Figures/Tables 2

References 16

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Research on benzaldehydes from the soft coral-associated symbiotic fungus Aspergillus sp. EGF15-0-3

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