中国南海豆荚软珊瑚Lobophytum sp. 的化学成分及其抗菌活性研究

doi:10.11978/2023132

Abstract

Abstract:

To investigate the chemical composition and biological activity of the soft coral Lobophytum sp. collected from South China Sea. A series of methods such as silica gel column chromatography, thin-layer chromatography, Sephadex LH-20 column chromatography, and semi-preparative high performance liquid chromatography were used to separate and purify. The structure of the compounds was characterized by analysis of NMR data and comparison with the data reported in literature. Twelve compounds were identified from the soft coral Lobophytum sp., including nine diterpenoids: (+)-(7R, 8R)-epoxy-7, 8-dihydrocembrene C(1), (1R, 2E, 4R, 7E, 11E)-cembra-2, 7, 11- triene-4-ol(2), (-)-(2R, 7R, 8R)-sarcophytoxide(3), entsarcophine(4), sarcotenusene C(5), (7S, 8S)-epoxy-1, 3, 11-cembratrien-16-oic methyl ester(6), sarcophytonin B(7), sarcoconvolutum D(8), laevigatol A(9); three steroids: campesterol(10), (24S)-24-methylcholesterol(11), gorgonol(12). Compound 8 was isolated from the soft coral Lobophytum sp. for the first time. The bioassay results revealed that compounds 3, 4, 5, 7 and 10 show significant antibacterial activity against fish pathogens Photobacterium damselae FP2244, oxytetracycline-resistant Streptococcus parauberis SPOF3K, and human pathogen vancomycin-resistant Enterococcus faecium.

Key words: soft coral, Lobophytum sp., diterpenoid, steroid, antibacterial activity, drug-resistance bacteria

SUN Manman, ZENG Yanbo, XU Han, YAO Ligong, GUO Yuewei, SU Mingzhi. Chemical composition and antibacterial activities of the soft coral Lobophytum sp. from the South China Sea[J].Journal of Tropical Oceanography, 2024, 43(4): 189-197.

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References 22

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菌种	化合物MIC
菌种	3	4	5	7	10	Tetr	Oxy	Lev	Amp	Van
vancomycin-resistant E. faecalis G1	-	-	-	-	38.7	0.09	0.19	>39.78	>37.14	>297
vancomycin-resistant E. faecalis G4	-	-	-	-	38.7	0.19	0.19	>39.78	>37.14	>297
vancomycin-resistant E. faecalis G7	30.2	31.6	33.2	1.5	0.48	0.09	0.09	>39.78	>37.14	18.56
vancomycin-resistant E. faecalis G8	-	-	-	-	38.7	0.09	0.05	39.78	>37.14	74.25
A. salraonicida AS42	-	-	-	-	-	6.11	0.39	0.31	>18.57	NT
E. piscicida TH1	-	-	-	-	-	1.5	0.78	0.62	9.28	>148.5
P. damselae FP2244	30.2	31.6	33.2	30	38.7	0.02	0.02	0.02	0.02	NT
oxytetracycline-resistant S. parauberis SPOF3K	30.2	-	-	30	38.7	>24.05	12.42	1.24	0.58	NT
S. agalactiae WR10	-	-	-	-	-	0.75	0.78	2.49	1.16	<0.2
V. algaelyticus RZ53	-	-	-	-	-	1.5	1.55	0.31	>37.14	18.56

Chemical composition and antibacterial activities of the soft coral Lobophytum sp. from the South China Sea

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