Journal of Tropical Oceanography ›› 2017, Vol. 36 ›› Issue (6): 100-108.doi: 10.11978/2017043CSTR: 32234.14.2017043

Special Issue: 南海专题

• 海洋生物学 • Previous Articles     Next Articles

Identification and phylogenetic analysis of Sagittidae (Chaetognatha) in the South China Sea based on mitochondrial CO#cod#x02160; gene

Bin CHEN(), Wei HE, Haitao LI, Peng WU, Yuzhang Xiao, Xiangli LYU(), Jing HE   

  1. South China Sea Environmental Monitoring Center, State Oceanic Administration, People#cod#x02019;s Republic of China, Guangzhou 510300, China
  • Received:2017-04-11 Revised:2017-06-08 Online:2017-11-30 Published:2018-01-18
  • About author:

    Author:QIU Chunhua.E-mail: qiuchh3@mail. sysu.edu.cn

  • Supported by:
    Guangdong Natural Science Foundation (2014A030310495);Director Fund of Marine Science and Technology, South China Sea Branch, State Oceanic Administration, People#cod#x02019;s Republic of China (1513)

Abstract:

To improve the accuracy of Chaetognatha identification, we combined morphological identification and DNA barcoding based on the mitochondrial CO#cod#x02160;(cytochrome oxidase subunit #cod#x02160;) gene to identify some arrow worms from the northern South China Sea. The results showed that the morphologically and molecularly identified results were consistent, for the species of Ferosagitta robusta, Aidanosagitta neglecta and Aidanosagitta regularis. Furthermore, the neighbor-joining phylogenetic tree was constructed with the amplified CO#cod#x02160; sequences and those homologous sequences from GenBank based on Kimura 2-parameter, and the phylogenetic positions of Sagiitta (Sagittidae, Sagittoidea) were discussed. The results indicated that these CO#cod#x02160; sequences formed monophylotic clades compared with GenBank (91%~100%). The intraspecific genetic distance ranged from 0.000 to 0.015 with an average value of 0.004, while the interspecific genetic distance ranged from 0.081 to 0.173 with an average value of 0.127. The barcoding gap was detected between intra and interspecific variations, suggesting that the CO#cod#x02160; gene could be a standard DNA barcode for the Chaetognatha identification. This study was the first to apply DNA barcoding to phylogenetic analysis of Sagittidae, and to discuss the phylogenetic position at molecular level.

Key words: DNA barcoding, CO#cod#x02160;, gene, phylogeny, Chaetognatha, Sagittidae

CLC Number: 

  • Q178.53