杂交石斑鱼Epinephelus fuscoguttatus♀×E. lanceolatus♂血细胞的类型及发生

doi:10.11978/2017006

Abstract

Abstract:

To study the blood cell morphological classification and production characteristics of hybrid grouper Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂, peripheral blood smears and tissues stamps (pronephros, kidney, spleen and liver) were made; cell staining and microscopic observation, differential leucocyte counts (DLC) of peripheral blood and observation of various types of blood cell development in the four types of tissues were conducted, and the results were compared with its female parent. The results showed that lymphocytes [(55.46±12.38)%], thrombcytes [(21.11±5.38)%], neutrophils [(19.44±12.02)%], monocytes [(1.66±2.13)%] and eosinophils [(2.33±1.91)%] were observed in peripheral blood. The pronephros showed the highest immature cells percentage [(16.12±0.30)%], followed by kidney [(7.90±0.35)%], spleen [(2.56±0.21)%] and liver [(0.97±0.18)%]. The results indicated that the major hematopoietic tissue of hybrid grouper was pronephros, followed by kidney. Besides, plasma cells were observed in the four types of tissues, basophils and macrophages were observed in stamps of pronephros, kidney and spleen, and huge primitive monocytes were observed in kidney stamps. Compared with the female parent, the ratio of lymphocytes in peripheral blood leukocytes was similar (+2.16%), with less thrombotic cells (-14.58%), and more neutrophils (+9.10%), eosinophils (+2.18%) and monocytes (+1.38%). The main hematopoietic tissues of hybrid grouper and its female parent were both pronephros, but the major tissues for lymphocytes production were different (kidney and pronephros, respectively); and the secondary hematopoietic tissues were different ((kidney and spleen). The proportion of immature cells in the tissues of the hybrid grouper was significantly higher than that of its female parent, showing active hematopoietic activity. The results of this study provide basic data of morphology classification and blood cell production for studying blood physiology, pathology of grouper and hybrid breeding of grouper.

Key words: hybrid grouper E. fuscoguttatus♀×, E. lanceolatus♂, blood cell, blood cell types, tissue, blood cell production

Hui ZHOU, Gang CHEN, Chongxin DENG. Blood cell types and production of hybrid grouper E. fuscoguttatus ♀×E. lanceolatus ♂[J].Journal of Tropical Oceanography, 2017, 36(5): 49-58.

Figures/Tables 6

Tab.1

Tab.2

Fig. 1

Tab. 3

Percentage of different blood cell lines in four tissues of hybrid grouper"

细胞类型	头肾		脾脏		肝脏		体肾
细胞类型	个数	百分比/%	个数	百分比/%	个数	百分比/%	个数	百分比/%
原红细胞PE	26.43±10.74	0.81±0.39	0	0	0	0	5.43±1.99	0.21±0.10
幼红细胞IE	191.43±23.98	5.85±0.43	0	0	0	0	22.43±14.35	0.87±0.47
红细胞 Er	278.86±62.50	8.53±1.82	968.00±31.21	71.24±2.42	3402.71±163.71	90.28±1.85	1010.14±286.53	38.83±2.06
原粒细胞PMy	30.57±17.65	0.93±0.26	1.29±1.38	0.09±0.04	0	0	7.57±4.86	0.29±0.24
早幼粒细胞Pr	35.14±10.12	1.07±0.26	2.43±2.51	0.18±0.22	0	0	12.71±12.61	0.49±0.23
嗜中性中幼粒细胞 MIN	19.43±13.15	0.59±0.32	0	0	0	0	7.57±4.65	0.29±0.24
嗜中性晚幼粒细胞 LIN	60.00±18.10	1.83±0.21	0	0	0	0	9.00±6.76	0.35±0.23
嗜中性粒细胞Ne	118.00±17.08	3.61±0.41	17.57±6.48	1.29±0.24	2.29±5.62	0.06±0.19	26.86±11.22	1.04±0.31
嗜碱性粒细胞Ba	4.14±3.93	0.13±0.13	3.86±4.35	0.28±0.33	0	0	1.43±1.40	0.06±0.06
嗜酸性粒细胞Eo	15.00±4.00	0.46±0.15	58.86±13.53	4.33±0.53	8.14±0.90	0.22±0.24	13.57±8.32	0.53±0.30
原淋巴细胞PL	37.29±6.90	1.14±0.32	7.00±2.31	0.52±0.04	1.00±1.73	0.03±0.08	33.29±8.01	1.29±0.21
幼淋巴细胞IL	39.86±13.84	1.22±0.45	14.29±1.38	1.05±0.12	1.43±2.57	0.04±0.07	71.86±10.32	2.78±0.74
大淋巴细胞LL	25.86±9.26	0.79±0.14	5.00±1.53	0.37±0.14	1.60±4.58	0.04±0.29	8.29±2.43	0.32±0.21
小淋巴细胞SL	1707.86±701.32	52.21±2.20	184.00±21.40	13.54±1.32	248.57±35.88	6.60±0.41	1214.71±380.44	46.90±2.27
浆细胞Pl	92.19±4.08	2.82±0.13	10.29±1.13	0.76±0.10	6.43±1.29	0.17±0.28	86.29±9.88	3.34±0.37
原单核细胞PMo	30.43±6.55	0.93±0.25	4.86±3.71	0.36±0.28	2.57±3.15	0.07±0.07	7.71±6.21	0.30±0.20
幼单核细胞Imo	56.57±19.89	1.73±0.25	4.86±4.77	0.36±0.36	31.29±6.68	0.83±0.47	26.71±11.56	1.03±0.72
单核细胞Mo	15.14±4.56	0.46±0.2.	6.86±1.19	0.50±0.17	25.57±8.98	0.68±0.82	14.14±3.76	0.55±0.25
血栓细胞Th	486.71±30.21	14.88±0.97	69.57±13.70	5.12±0.97	37.29±8.74	0.99±0.23	15.43±3.91	0.60±0.34

Tab. 3

Tab. 4

Tab. 5

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细胞类型	细胞大小		核大小
细胞类型	长径/µm	短径/µm	长径/µm	短径/µm
红细胞	12.35±1.45	6.21±0.51	4.53±0.32	3.41±0.18
大淋巴细胞	7.18±1.49	5.36±0.93	5.08±1.00	3.77±0.53
小淋巴细胞	5.74±0.62	4.53±0.39	4.86±0.64	3.73±0.49
血栓细胞	10.47±1.03	3.69±0.95	7.58±0.99	2.97±0.82
嗜中性粒细胞	9.82±1.43	7.50±0.92	6.10±0.87	4.61±0.61
嗜酸性粒细胞	7.42±1.05	6.32±0.98	4.85±0.50	4.24±0.61
单核细胞	13.21±1.12	11.87±1.32	8.98±0.68	7.44±1.12

组织	红细胞	淋巴细胞	粒细胞	单核细胞	总数
头肾	6.66±0.40	2.36±0.34	4.44±0.24	2.66±0.23	16.12±0.30
体肾	1.08±0.29	4.07±0.44	1.42±0.23	1.33±0.46	7.90±0.35
脾脏	0	1.57±0.14	0.27±0.13	0.72±0.33	2.56±0.21
肝脏	0	0.07±0.07	0	0.90±0.31	0.97±0.18

细胞系	细胞大小		细胞核大小
细胞系	长径/µm	短径/µm	长径/µm	短径/µm
原红细胞 PE	9.31±0.74	7.65±1.28	6.99±1.06	5.61±0.93
早幼红细胞 EIE	7.79±1.26	6.60±0.71	5.21±0.49	4.72±0.64
中幼红细胞 MIE	8.15±0.65	6.60±0.94	5.15±0.55	4.46±0.48
晚幼红细胞 LIE	8.62±0.92	5.57±0.91	5.06±0.56	3.89±0.47
红细胞 Er	12.26±1.10	6.38±0.48	4.70±0.38	3.29±0.21
原粒细胞PMy	10.32±1.40	8.75±1.43	7.41±0.68	6.46±1.06
早幼粒细胞Pr	9.54±0.73	8.86±0.55	7.08±0.53	5.95±0.51
嗜中性中幼粒细胞MIN	10.34±1.23	9.01±1.28	6.81±0.74	5.61±0.74
嗜中性晚幼粒细胞LIN	9.15±0.81	8.40±0.72	6.13±0.32	5.36±0.54
嗜中性粒细胞Ne	10.39±0.70	9.78±0.69	5.84±0.48	5.16±0.37
嗜碱性粒细胞Ba	8.91±0.41	8.01±0.60	6.11±0.65	5.19±0.59
嗜酸性粒细胞Eo	7.72±0.66	6.77±0.69	5.53±0.47	4.77±0.38
原淋巴细胞PL	7.76±0.99	6.31±0.76	6.24±0.84	5.33±0.75
幼淋巴细胞IL	7.08±0.64	5.64±0.39	5.92±0.52	5.19±0.40
大淋巴细胞LL	6.86±1.37	5.97±0.93	5.20±1.16	4.74±0.80
小淋巴细胞SL	6.12±0.78	5.32±0.73	5.03±0.57	4.16±0.50
浆细胞Pl	10.04±1.47	8.68±0.94	6.09±0.69	4.91±0.74
原单核细胞PMo	9.37±1.16	8.44±1.65	7.62±0.78	6.08±1.08
幼单核细胞Imo	11.72±0.99	10.92±0.67	8.53±1.17	5.75±0.87
单核细胞Mo	13.20±1.57	10.37±1.25	8.15±1.34	6.46±0.96
血栓细胞Th	4.47±0.21	3.75±0.28	4.24±0.16	3.72±0.31
巨噬细胞 Ma	15.19±1.93	11.29±1.73	8.14±0.48	6.87±0.69

Blood cell types and production of hybrid grouper E. fuscoguttatus ♀×E. lanceolatus ♂

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细胞类型	个数	百分比/%
淋巴细胞	119.00±30.18	55.46±12.38
血栓细胞	45.29±9.01	21.11±5.38
嗜中性粒细胞	41.71±22.13	19.44±12.02
嗜酸性粒细胞	5.51±5.00	2.33±1.91
单核细胞	3.57±3.99	1.66±2.13

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