海南澳洲管体星虫卵细胞发育及生殖周期研究

doi:10.11978/2021143

Abstract

Abstract:

Siphonosoma australe is an important sipuculan species and a typical local resource in Hainan with medicinal and edible value, it is distributed in tropical and subtropical coastal waters, mainly in surrounding waters of Hainan island. Due to overfishing and habitat destruction, the population size of S. australe rapidly declined in recent years. So the recovery and protection of the sipuculan resource is very important. The structures of oocytes of S. australe were observed by optical microscope and electron microscope. The results are shown as follows: 1) The oogenesis of S. australe could be divided into four stages: In the early growth stage, concave disk-shaped coelomocytes (less than 30 μm in diameter) changed from the smooth cells to lumpy and corrugated cells, which suggested that concave coelomocytes were the oogonia; in the late growth stage, the surface of 30 ~ 40 μm oocytes became smooth and unfolded, and the vitelline membrane of 1 μm in thickness appeared with the accumulation of yolk granules; in the early mature stage, oocytes were 60 ~ 120 μm in diameter and transformed into spherical shape. The yolk granules increased. The nucleus was enlarged. Some of the chromatin formed clumps with high electron density and dispersed in the nucleus; in the late mature stage, oocytes were 120 μm in diameter with the yolk granules filling the whole cells, and the membrane pores were exposed on the membrane surface. 2) The vitelline membrane of mature oocyte can be divided into two layers: the homogeneous inner layer with a thin thickness and the multi-folded outer layer with granular protuberances. The structure of membrane micropore was simple and formed by the invagination of the folded membranes. 3) During oogenesis, a variety of organelles increased and the yolk granules (type Ⅰ and Ⅱ) were combined with organelles, e.g. mitochondria, endoplasmic reticulum, Golgi apparatus, lysosome, evenly transformed by phagocytic vesicle. The type Ⅱ yolk-granules enveloped by Golgiosomes were common. 4) The reproductive season of S. australe in Wenchang, Hainan is from April to August and the reproductive peak is in May and July. Oocytes begin to appear in early March, quantitatively drop to a very low level in September and disappear in October. The mature oocytes keep a certain density in the coelom from April to August. It is speculated that the reproduction period is related to the monthly water-temperature and the season of highest water-temperature is the period of reproductive peak. As a new Hainan fishery resource with tropical characteristics, this research on oocyte development and reproductive cycle of S. australe is expected to greatly promote the development on related technologies for reproduction and protection.

Key words: Siphonosoma australe, Hainan, oogenesis, microstructural observation, ultrastructural observation, reproductive cycle

CLC Number:

S917

QIAO Lijun, YAO Xuemei, YU Qiaochi. Development of oocytes and reproductive cycle of Siphonosoma australe in Hainan[J].Journal of Tropical Oceanography, 2022, 41(5): 161-169.

Figures/Tables 5

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References 22

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Development of oocytes and reproductive cycle of Siphonosoma australe in Hainan

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